FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L33_GCTACGCT-TATCCTCT_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L33_GCTACGCT-TATCCTCT_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2859929
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG8050.022.4176355
TAGACAG7950.022.1022595
CTATACT10000.021.3704954
CTACACT13850.019.8875034
ACTGTGC10050.019.8486448
GTCCTAT4950.019.186531
TCTATAC9600.018.7981193
GTATAAG8350.018.7671851
TACACAG11150.018.7404425
GTACTAG5650.018.4903831
TACACTG15600.018.2653795
CTTACAC8750.017.9105113
GTATAGG9150.017.6453191
CCTACAC10500.017.639143
GTATTAG9050.017.315581
TATACAG12150.017.198025
CTAGCAC4507.2759576E-1216.8853283
GTCCTAC3151.3460885E-716.5826441
ACCACGT13250.016.488828
GTCCAGG6050.016.4829751
Produced by FastQC (version 0.11.2)