FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L33_GCTACGCT-TATCCTCT_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L33_GCTACGCT-TATCCTCT_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2859929
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACAG9950.019.5726345
CTATACT9050.019.419664
GTGTAGA16850.018.6233561
TACACCG8500.017.8821435
TAATACT15050.017.6742124
GTGTAAG10000.017.5920161
AGTCCCG3251.00390025E-817.5382565
CTACACT16250.017.5382544
GTGTAGG9300.017.3824061
TCTATAC9600.017.3184133
TACACTG16200.017.299185
AGGAGTG8800.017.2725245
TATACCG7450.017.2145615
TAAGGGT6700.017.0147254
ACTGTGC11250.016.8886898
GTGTAGC8500.016.7809371
CCTGTAC6800.016.7653883
TGGACCG5700.016.6664715
CTAGCAC4358.0035534E-1116.3799763
CTACACA13350.016.3668484
Produced by FastQC (version 0.11.2)