FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L32_CAGAGAGG-TATCCTCT_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L32_CAGAGAGG-TATCCTCT_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1048551
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGTG1554.5980414E-827.5736129
CTACACA4550.025.047364
CTATACT3350.024.097184
GGACCGT1201.7141917E-423.7428116
GTATTAG3300.023.018941
TACACAG4000.022.5556685
GTAGGGC1903.2959724E-722.4931873
CTAACAC3650.022.116593
TAAGACA3550.021.401974
CCTATAC2951.3460522E-1020.9258673
TAGGGCA2509.5205905E-920.8936734
AGGACCG1150.003729599320.6459225
ATAAGAC4200.020.350983
ACACAGT4950.020.1454146
ACTGACC2851.9681465E-919.9958538
GCTAGGG1200.00477157519.7856752
TACTAGC1200.00477157519.7856752
TAGAGCG1456.1580876E-419.6492215
GTGTATA5100.019.5491711
ATAGGAC2201.3484241E-619.4259363
Produced by FastQC (version 0.11.2)