FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L32_CAGAGAGG-TATCCTCT_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L32_CAGAGAGG-TATCCTCT_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1048551
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG2201.8189894E-1228.0679155
TAGACAG2900.027.844565
CCCTAGG1056.876623E-527.1594351
CTAGTAC1457.877279E-726.2103943
TACACGG2007.185008E-1026.124755
TATACAG5200.024.6632255
GTAGCAC2704.0017767E-1122.8734263
AGGGGTG2103.4458935E-822.6188345
GTATAGA3059.094947E-1221.8165951
ACACGGT2901.05501385E-1021.29296
CCTAGGA1805.1620646E-621.113932
TATACTA3900.020.7049315
GTACAGG3252.1827873E-1120.4740351
GTGTTAG3503.6379788E-1220.3695771
GTGCTAC3102.564775E-1019.9315221
CTTAGCG1200.004763487719.7914779
GAGTAGG3153.1832315E-1019.6151471
GTGTAGA5750.019.0116061
TGCTACA4001.8189894E-1219.0025392
CTAGCAC1507.7135104E-419.0025373
Produced by FastQC (version 0.11.2)