FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L31_CTCTCTAC-TATCCTCT_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L31_CTCTCTAC-TATCCTCT_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1452915
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT3900.025.5662464
GGACCGC1553.9199356E-521.4426576
CTGTCCC3650.020.8182439
GTACAGG3052.0918378E-1020.2367531
GTATAAG4300.019.8747371
CCGTCCC1707.9664955E-519.555379
CCTGTAC2502.1825872E-718.992073
GTGCTAG2752.8536306E-818.9914151
GTCTTAC3054.5911293E-918.6800791
CTGTGCG4101.8189894E-1218.5333129
GTGTAAG5150.018.4382671
TCCTATA4650.018.3794212
GCTGTCC4750.017.9974428
TGTACCG3451.0895747E-917.8910795
TAGGGAG4002.3646862E-1117.8050655
GTAGGGC1600.001194560417.8050633
ACAGTCC2704.882877E-717.5900948
GTATAAT8450.017.4181611
ATTAGGG3007.7207005E-817.4093953
GTATTAT10800.017.1450271
Produced by FastQC (version 0.11.2)