FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L31_CTCTCTAC-TATCCTCT_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L31_CTCTCTAC-TATCCTCT_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1452915
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG6450.025.0385325
GCCCTAT2751.8189894E-1224.197421
TAGGGTG1801.9519393E-723.7497845
CTACACT6100.022.5817624
GTATAAG4850.021.5602951
TACACAG5350.021.3082185
GTGTAGG4950.021.1247311
CTAATAC4200.020.359063
GCTACAC3603.6379788E-1219.7935313
GTATTAG4201.8189894E-1218.1069151
CTATACT4201.8189894E-1218.0950744
GTCTTAG2704.84275E-717.6039451
GCCCTGG3259.946234E-917.5497781
TGGACCG3251.002627E-817.5383015
TAGGGTC2453.7634054E-617.4488225
TTAGGGT3551.5843398E-917.3942094
GTACTAG2755.869206E-717.2838731
TTATACT8550.017.2220674
CTAAGGA1952.2759914E-417.0511284
GTCCAGG3652.2719178E-916.9287241
Produced by FastQC (version 0.11.2)