FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L294_TCCTGAGC-TATCCTCT_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L294_TCCTGAGC-TATCCTCT_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1104046
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTGCG2051.8189894E-1230.116859
GTATGCG1000.001657418623.74061
GTCTTAG2657.9671736E-1021.500921
GGACCGC1353.8048095E-421.103716
GGGTATG1605.004292E-520.7730241
CTAGGAC2309.05311E-820.6449363
CCGCACG1856.561315E-620.5370649
TAGCCGC1856.571612E-620.533346
TAAGGTG2451.7623461E-719.3809595
GGTGTGC3003.7216523E-918.9976438
AGGGTCC1507.727231E-418.9976438
CTGTCCG2251.6702143E-618.9967849
TACGGGT1507.739014E-418.993344
GGGTGTG3305.984475E-1018.7089527
GTGTGCG3851.2732926E-1118.5033599
CCAGGGG1559.646876E-418.3806513
ACTGTGT5450.018.3004848
GTCCTAC1851.525885E-417.9658581
CGTGGGC1600.001193777817.8062553
AGGGGTG3208.305506E-917.8062555
Produced by FastQC (version 0.11.2)