FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L294_TCCTGAGC-TATCCTCT_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L294_TCCTGAGC-TATCCTCT_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1104046
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG2400.027.7079735
CTATACT3700.026.9591144
CCGCTCG950.001226616424.9996789
GCTACAC3100.024.5191463
GCGTTAT1452.3173574E-522.952571
GTGTAGG3400.022.3739341
GTGTAGC2606.184564E-1021.9436661
ACTGCCC1754.049456E-621.7140068
GTGTACG2005.3372787E-721.3950751
ACCACGC2005.386355E-721.3747258
CTACACT5600.020.356884
TACACAG3506.366463E-1118.9997565
CTAGTAC2302.055287E-618.5892433
ACTATAC4350.018.5655023
ACTAAGC2051.5876183E-518.5388643
TATACCG1801.2333866E-418.4719835
TATACTG8350.018.203365
TCTATAC3156.7939254E-918.0974643
GTCTAGC2403.0623196E-617.8292271
TCTACAC7300.017.5706543
Produced by FastQC (version 0.11.2)