FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L28_AGGCAGAA-TATCCTCT_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L28_AGGCAGAA-TATCCTCT_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1409663
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG3000.023.7436125
GTATAGA4700.023.2343041
CTAGCAC2303.6397978E-922.711283
GTCCTAC2005.411057E-721.3654611
AGTGTCC4700.021.2199548
AGCACCG3950.020.437545
GTGCTAC4050.019.9293731
ACCACGT6000.019.7884528
GTGTTAG4800.019.7828331
GTCCTAG2401.4204124E-719.7828331
ACTGTCC4400.019.4286618
CTACACT7550.018.8690954
GTGTATA6100.018.6801831
CTATACT5200.018.2643174
TGGTCGC11150.018.11272494-95
CCCTTGG2101.9638377E-518.0871621
CTGCGGT1851.5243683E-417.9687779
TAGACAG5600.017.8077095
TCTACAC9100.017.742483
TAGACCG1650.001466674717.2680825
Produced by FastQC (version 0.11.2)