FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L28_AGGCAGAA-TATCCTCT_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L28_AGGCAGAA-TATCCTCT_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1409663
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG8950.026.0052765
CTACACT8900.023.4828664
CCTGCAC2257.1500835E-821.1123583
CTAAGAC3052.0918378E-1020.2470973
GTGCATA5050.019.7711731
ACTGTCC3850.019.7400238
GTGCTAC5800.019.6737791
GCCTTAC1707.8978504E-519.577341
ACCGCGC1707.9562145E-519.558598
ACCACGT6800.019.558598
GTGCTAG2502.1513733E-719.0179861
CTGATCG3003.7180143E-918.9997739
CTGTGCG2752.8390787E-818.9997739
ACAGTCC3357.3123374E-1018.4326158
CTCCTAG2854.228059E-818.3506891
CCTAAGA2854.269532E-818.3350642
TAACACT6500.018.2690124
ATACCGT3901.4551915E-1118.2690126
GTGTAGG5500.018.1535321
CCGCTCA2905.216134E-818.0170279
Produced by FastQC (version 0.11.2)