FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L24_AAGAGGCA-CTCTCTAT_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L24_AAGAGGCA-CTCTCTAT_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1683657
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTAT1658.4550265E-825.8964271
GTCCTAC1401.786866E-523.7383941
CTATACT4150.022.8824184
GTCCTAT2554.947651E-1022.3420161
TAAGGGT2858.367351E-1121.6580094
CTATGCG2005.4037446E-721.3689989
GTATTAG4300.020.9781151
CCCTAGG1150.003732620520.643922
TATACCG2351.13743226E-720.2046875
GTATTAT8250.020.1416681
CTAGTAC2601.4981197E-820.0881213
TACAGCG1908.270801E-619.9955717
CCTATAC2851.9754225E-919.9920083
GTCGCCG22350.019.88025594-95
CTAAGCG1456.164235E-419.6473183
GGTCGCC26400.019.35051294-95
TCGCCGT12900.019.15585594-95
ACCGCTC2502.1785854E-718.9957928
ACTGTCC3759.094947E-1218.9957928
TAGGTCG1507.7438826E-418.9924075
Produced by FastQC (version 0.11.2)