FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L24_AAGAGGCA-CTCTCTAT_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L24_AAGAGGCA-CTCTCTAT_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1683657
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTGC4750.021.9997548
GTGTAGC3059.094947E-1221.820531
CTTAGGG1553.9036757E-521.4545572
CTAAGAC2457.516064E-921.3288233
GTACTAG2709.931682E-1021.1278151
GCTATAC1805.166292E-621.1133823
GTATAAA10750.020.3416631
CTTCTGC43100.020.333394-95
CTATACA4450.020.280674
GACTGTG3052.0918378E-1020.2456747
TAGACAG3455.2750693E-1119.2751455
GTCCTAA2502.1555206E-719.0150321
TACACTG6050.018.8427625
GTATTAG4800.018.8169611
CCTGTGC3305.984475E-1018.711918
ATACCGT4600.018.5867486
CTAAGGA2051.5902631E-518.5363774
TACACAG3851.2732926E-1118.5062855
TGCTAGG1801.2324555E-418.4747562
CTAGGGT1801.2338592E-418.4720134
Produced by FastQC (version 0.11.2)