FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L23_GCTACGCT-CTCTCTAT_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L23_GCTACGCT-CTCTCTAT_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences631041
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTAAG1452.4001565E-829.4779382
ACTGTCC1701.1220618E-725.1449418
AGCGTGC1401.7789902E-523.7479978
TAACGGT1000.001654395323.7461174
CTGTGCG1801.9512117E-723.7461179
GTATATA3600.023.7423531
CCTTACG1050.002199243322.6117671
CTATACT1903.2872413E-722.4963234
TCTATAC1903.2872413E-722.4963233
CTAACAC1703.1497202E-622.3492873
TAGGGCT1302.9427256E-421.9194934
ACCGTGA1553.905365E-521.4498048
GTGTTGG3101.0913936E-1121.4447081
TATAAGG2257.151175E-821.1076582
TAGGACT1150.00372497120.6487984
GTATTAG2309.0349204E-820.6455251
TAAGACA2108.608549E-720.3538154
GTGTAAG2351.13368515E-720.2062591
ATAGGGC1200.004763482619.7899978
ATACCGA2401.4130819E-719.788436
Produced by FastQC (version 0.11.2)