FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L23_GCTACGCT-CTCTCTAT_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L23_GCTACGCT-CTCTCTAT_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences631041
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGAC652.5862319E-643.84933
CCTTAGG902.4100684E-531.668942
GTGTAAG2000.030.8919051
GTCTAGC1309.969299E-625.5909271
TAGGACC950.001225995424.9998114
TACACCG1751.4840407E-724.4283895
CTTTATA6600.023.7631
CTTAGTC1000.001652131423.7517053
TCTATAC1801.9467734E-723.7517053
CTGTCCC2002.0505468E-823.7498239
GACTGTA1601.8574628E-623.7498237
TAGGACT1201.7097122E-423.749824
ACTGCGC1452.3323206E-522.9308628
GGGTGGC1252.2544526E-422.799836
GTGTACC1503.0176354E-522.17881
CTATACT2154.4139597E-822.0928574
CTAACAC1954.2122883E-721.9246483
CTATAGT2606.2391337E-1021.9229134
GACTGCG1302.9396225E-421.9229137
GCTGTCC2405.9153535E-921.770678
Produced by FastQC (version 0.11.2)