FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L239_CGAGGCTG-CTCTCTAT_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L239_CGAGGCTG-CTCTCTAT_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences609356
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTGGT459.631047E-442.213722
GCGATAC500.001616817937.992353
CCCCTGG500.001617469937.9892351
CTCCTAG1001.3007411E-633.2405781
GACCGTG753.080398E-431.6602927
GTCCAGG1201.5075966E-731.6576941
GTATAGG1703.6452548E-927.933261
ACCGTGC700.0083967727.1373948
TAGACTG2007.185008E-1026.1197415
CTGTCCT950.001227332224.9949689
GTCCTAA950.001227919624.9929181
CTAATAC2101.2660166E-924.8759443
CCTGGAC1201.7118659E-423.7452183
TACACCG1201.7118659E-423.7452185
ACACTGG1852.5425106E-723.1034556
TGCTAGG1050.002197614922.6144942
GCCTTAC1050.002198663322.6126381
CCTATAC2405.9280865E-921.766453
GTATAGT2405.9335434E-921.7646641
TAGCACT1754.051144E-621.7099154
Produced by FastQC (version 0.11.2)