FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L239_CGAGGCTG-CTCTCTAT_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L239_CGAGGCTG-CTCTCTAT_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences609356
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGCA801.0756305E-535.630542
TATACCG1303.0331466E-729.2305155
ACCGCGC700.00838888527.1426228
CAGTGCG1257.38394E-626.599779
CTAGCAC908.9388737E-426.3908253
TAACACT3100.026.048164
GTCGTGG1109.433096E-525.923761
CTAACAC1901.1878001E-825.0018353
TCCTATA2101.2605597E-924.884822
GTCCAAG1351.3355109E-524.6435741
GTGTTAG1551.4018915E-624.5300081
GTCTAGA1551.4018915E-624.5300081
GCCTAGC1000.001647320823.7634451
CTTAGTA1401.7755636E-523.7536932
ACACCGT1401.7778208E-523.7497946
CTATACC1401.7778208E-523.7497944
ACAGTGC2452.9649527E-1023.2651068
CTGTCCC2052.6642738E-823.170539
CTCTACG1050.002187837622.6318511
GTCTATG1703.1295476E-622.3655951
Produced by FastQC (version 0.11.2)