FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L22_CAGAGAGG-CTCTCTAT_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L22_CAGAGAGG-CTCTCTAT_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1452351
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGT4150.022.8855746
GTATTAG5200.021.915831
GTCCTAC2005.4052725E-721.3679331
TACACAG6050.020.407885
TAGACAG4050.019.9330545
TGGGCCG2401.4177385E-719.7864865
AGGACCG1456.157951E-419.6500285
TAGGACC2251.6724352E-618.9950284
CCTATAC5450.018.2979623
GGGCCGT2603.2905882E-718.264456
GCGTTAT2101.9619507E-518.0892561
TACACCG4500.017.9397495
CTATACT6400.017.8078384
CTAGTAC2956.360642E-817.7072283
CTTAGGG1350.0094157417.5879883
ACTGTGC5450.017.4296328
GTAGCAC6350.017.200223
ACCGTCC2504.565778E-617.0984678
GGTCTAT2253.548676E-516.8833051
GTGTTAC4801.8189894E-1216.8173541
Produced by FastQC (version 0.11.2)