FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L22_CAGAGAGG-CTCTCTAT_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L22_CAGAGAGG-CTCTCTAT_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1452351
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGGT1601.8602714E-623.7497774
CCTAGTA2657.930794E-1021.5114542
ACCACGT7150.021.2585418
GTATATA9850.021.2326951
CTGACAC7450.021.0422443
ACCGTGC3700.020.5403488
GTATACA6400.019.3099881
TATACCG4001.8189894E-1218.999825
TACACCG4550.018.7910335
GTCTAAG3609.458745E-1118.4847761
TAGGGCC2101.9565356E-518.0950685
CCCTTGG2152.381186E-517.6864281
GCTACAC2704.870817E-717.5942443
TGACACC8100.017.5924264
GTGTAGA8450.017.4378781
CTGTGCG3903.0013325E-1017.051129
TACACAG5600.016.9641255
TCTACAC8150.016.9034273
GACACCA8250.016.6968125
CCGTGCG2858.587067E-716.666519
Produced by FastQC (version 0.11.2)