FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L200_CTCTCTAC-CTCTCTAT_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L200_CTCTCTAC-CTCTCTAT_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1308036
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACAC3750.022.7924213
AGGACCG1050.002200630722.6115265
ACTGTCC4050.022.2790998
ACCACGT6200.022.2129048
GTGTCCC2858.367351E-1121.661959
CCCCTGG1553.919292E-521.442841
CTGTCCC3800.021.2453739
TACACTG7900.021.0373065
CCTACAC4900.020.3503763
TATACCG3302.7284841E-1120.1448155
TACACAG5600.019.5024435
TCTAGCC2451.7626371E-719.381313
GTACACT6400.019.290464
CTATACT5450.019.1679364
CACCGTG2752.8452632E-818.9958617
TAGACCG1250.00604317218.9936855
GCTACCC1250.00604317218.9936853
CTAGGGG1759.9634926E-518.9936853
GCCCGGT1250.00604584218.9922311
CTACACT6800.018.8540254
Produced by FastQC (version 0.11.2)