FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L200_CTCTCTAC-CTCTCTAT_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L200_CTCTCTAC-CTCTCTAT_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1308036
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTTAG1951.5583282E-824.3764151
GTGCTAG1852.5247027E-723.1246531
TAGACCG1452.334859E-522.9307865
GTGTTAG3800.022.5161081
CCGTGCG1503.0338804E-522.1664289
TAGACTG3950.021.6453345
ATAGGGC2457.521521E-921.3262988
TAGGGCA2257.154449E-821.110889
CTAGGAC2951.3278623E-1020.9343763
TACACGG3001.6734703E-1020.583115
GCCATAC1856.5127824E-620.5552481
TATACAG5800.020.4739155
TACACCG3503.6379788E-1220.3569225
CTAAGCA2351.1323027E-720.2125474
CCCCTAG1456.117104E-419.6692451
ACCGCGT1707.955592E-519.558618
AGGACAG3003.7180143E-918.9997945
GGACCGC1250.006031956518.9997946
TAGGACG1250.006031956518.9997944
TATACGC2001.2850756E-518.9997945
Produced by FastQC (version 0.11.2)