FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L192_GGACTCCT-CTCTCTAT_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L192_GGACTCCT-CTCTCTAT_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences955104
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG2351.7826096E-1024.2415121
CCCAGGG2551.8189894E-1124.2031672
CTATACT2052.6828275E-823.1587544
TACCGCG3100.022.97687
TCCTAGG1452.3431563E-522.919182
CTTAGTC1050.002202560222.6073553
TTAGGAC1503.0446496E-522.1552073
GTCTTAC2154.4463377E-822.0804461
GTGTATA3301.8189894E-1221.5786171
GGTTAGC1100.002886535821.5786171
ACTGTGC3351.8189894E-1221.2643418
GTATATG3351.8189894E-1221.2565481
TAAGACC1805.1904317E-621.1001994
AGTACCG3151.4551915E-1121.1001975
CTAGGAC1856.5795557E-620.529923
CCTACAC3052.0918378E-1020.2354373
ACACTGC3800.019.9907076
TAGTACC1908.285793E-619.989664
CTACACA3353.45608E-1119.8404834
TTATACT4850.019.5775014
Produced by FastQC (version 0.11.2)