FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L192_GGACTCCT-CTCTCTAT_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L192_GGACTCCT-CTCTCTAT_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences955104
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG2550.031.6830231
CCCCTGG1652.6320777E-928.8027531
TCTATAC2850.025.001133
TTAAGGC1652.4282126E-623.0313453
TACACTG3800.022.4998385
TAGGAGT2755.2750693E-1122.4543864
GTCCTAA1703.1339787E-622.3644891
GTACACT3450.022.0288284
TATACCG1553.905747E-521.451465
GCTATAG1353.7820826E-421.1220171
TAGCCCG1353.7954812E-421.110965
CCTAAGA1604.9844082E-520.7832782
TAGCCAG1604.98843E-520.78115
TACACCG3700.020.5403945
CCTAGGA1404.8477217E-420.3591292
ATAAGAC2851.9608706E-920.0009043
TAGCAGG1200.00476311919.7915256
ACCTAGG1707.9205594E-519.5689281
ATAGGCC2451.7543971E-719.3886323
CTATACT2953.0140654E-919.3218964
Produced by FastQC (version 0.11.2)