FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L188_TCCTGAGC-CTCTCTAT_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L188_TCCTGAGC-CTCTCTAT_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31754
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAGT156.5968995E-494.859841
ATGGTCC200.00205465271.2570958
ACACAGT200.00206756771.1448756
ACCCTAG200.00206756771.1448751
TACGATT200.00206756771.1448754
TCTCGGG250.00160353938.00378894-95
TATATCG753.0382292E-431.6199465
GTGCTAT700.00833118127.1028141
CTATATC950.001210855224.9631164
GATCTCG1805.832135E-714.51533592-93
ATCTCGG1553.3917848E-513.79169794-95
AGATCTC1751.0584584E-412.21550392-93
TCTCGGT1400.003445620211.876183594-95
TAGATCT1902.276299E-411.25112190-91
TGTAGAT2351.4256944E-410.10738988-89
GTAGATC2150.0079979078.8380990-91
GGTGTAG2550.00337788.38318886-87
GAGGTGT2859.841251E-48.33416484-85
TAGAGAG4057.1423674E-68.21069480-81
AATAGAG4159.905896E-68.01284778-79
Produced by FastQC (version 0.11.2)