FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L188_TCCTGAGC-CTCTCTAT_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L188_TCCTGAGC-CTCTCTAT_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31754
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGTGC200.002055629271.248826
ATGCCGT2102.2202767E-713.57120394-95
TATGCCG2509.404175E-913.2997894-95
CGTCTTC1300.001978033512.7882594-95
CCGTCTT1300.001978033512.7882592-93
GTCTTCT1300.001978033512.7882594-95
CGTATGC2804.5803972E-811.87480492-93
TGCCGTC1801.3781869E-411.87480394-95
CTCGTAT2907.4580385E-811.46532790-91
GCCGTCT1500.00577189611.0831592-93
GTATGCC2704.622534E-610.55538192-93
TCGTATG2807.1364557E-610.17840390-91
GAGCATC3601.356384E-79.89566984-85
ATCTCGT3406.6755456E-79.7792588-89
GCATCTC3406.6755456E-79.7792586-87
CATCTCG3203.471522E-58.90610286-87
TCTCGTA3254.1658153E-58.76908688-89
CTGAGCA3853.6170204E-68.6362282-83
TCCTGAG3955.1114566E-68.41758280-81
AGCATCT3458.384152E-58.26073384-85
Produced by FastQC (version 0.11.2)