FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L182_AGGCAGAA-CTCTCTAT_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L182_AGGCAGAA-CTCTCTAT_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104401
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTCA250.005004018557.0129434
ACACCGA405.359536E-447.5107886
TGTACAG405.359536E-447.5107885
CTATTCT459.574108E-442.231814
TCTATAC459.574108E-442.231813
TAAAGCT550.00256607234.55334
CTCCTAT600.003948772331.643521
TTAGAGA804.460053E-429.6942424
GAACAGT804.460053E-429.6942426
AGTTGTG650.00581360629.2374065
CCATGTC650.005827250429.2233969
GTATTAC650.005840919429.2094021
GTACAAA650.005840919429.2094021
GTGTACT650.005840919429.2094021
GTGTAAC650.005840919429.2094021
GTATACA650.005840919429.2094021
TAGTTGT856.359449E-427.947524
TTATACT700.00834799427.1490214
CACACAG700.00834799427.1490215
TGTACTG700.00834799427.1490215
Produced by FastQC (version 0.11.2)