##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename G140_A6_L182_AGGCAGAA-CTCTCTAT_L006_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 104401 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.77638145228494 33.0 33.0 33.0 33.0 33.0 2 32.81110334192201 33.0 33.0 33.0 33.0 33.0 3 32.81902472198542 33.0 33.0 33.0 33.0 33.0 4 36.78112278618021 37.0 37.0 37.0 37.0 37.0 5 36.69977299068017 37.0 37.0 37.0 37.0 37.0 6 36.71722493079568 37.0 37.0 37.0 37.0 37.0 7 36.67101847683451 37.0 37.0 37.0 37.0 37.0 8 36.68607580387161 37.0 37.0 37.0 37.0 37.0 9 36.71682263579851 37.0 37.0 37.0 37.0 37.0 10-11 36.69263704370648 37.0 37.0 37.0 37.0 37.0 12-13 36.673460982174504 37.0 37.0 37.0 37.0 37.0 14-15 39.02431490119827 40.0 40.0 40.0 37.0 40.0 16-17 38.979152498539285 40.0 40.0 40.0 37.0 40.0 18-19 38.94408578461892 40.0 40.0 40.0 37.0 40.0 20-21 38.93112613863852 40.0 40.0 40.0 37.0 40.0 22-23 38.8856907500886 40.0 40.0 40.0 37.0 40.0 24-25 38.84390475187019 40.0 38.5 40.0 37.0 40.0 26-27 38.77154433386653 40.0 37.0 40.0 37.0 40.0 28-29 38.73358492734744 40.0 37.0 40.0 37.0 40.0 30-31 38.65883468549151 40.0 37.0 40.0 37.0 40.0 32-33 38.55610578442735 40.0 37.0 40.0 37.0 40.0 34-35 38.50878344077164 40.0 37.0 40.0 37.0 40.0 36-37 38.431609850480356 40.0 37.0 40.0 37.0 40.0 38-39 38.34679744446892 40.0 37.0 40.0 37.0 40.0 40-41 38.24559151732263 40.0 37.0 40.0 37.0 40.0 42-43 38.13315964406471 40.0 37.0 40.0 37.0 40.0 44-45 37.986211817894464 40.0 37.0 40.0 35.0 40.0 46-47 37.879153456384515 40.0 37.0 40.0 33.0 40.0 48-49 37.86969473472476 40.0 37.0 40.0 35.0 40.0 50-51 38.08564573136273 40.0 37.0 40.0 37.0 40.0 52-53 38.03327554333771 40.0 37.0 40.0 37.0 40.0 54-55 37.86598308445321 40.0 37.0 40.0 35.0 40.0 56-57 37.70026628097432 40.0 37.0 40.0 33.0 40.0 58-59 37.52137431633797 38.5 37.0 40.0 33.0 40.0 60-61 37.338722809168495 37.0 37.0 40.0 33.0 40.0 62-63 37.103514334153886 37.0 37.0 40.0 33.0 40.0 64-65 36.878947519659775 37.0 37.0 40.0 33.0 40.0 66-67 36.626900125477725 37.0 37.0 40.0 33.0 40.0 68-69 36.39986685951284 37.0 37.0 38.5 33.0 40.0 70-71 36.153020564937115 37.0 37.0 37.0 33.0 40.0 72-73 35.898118791965594 37.0 37.0 37.0 33.0 40.0 74-75 35.66088447428665 37.0 37.0 37.0 33.0 40.0 76-77 34.49804120650185 37.0 33.0 37.0 30.0 37.0 78-79 35.37329144356855 37.0 35.0 37.0 33.0 37.0 80-81 35.37187383262612 37.0 37.0 37.0 33.0 37.0 82-83 35.25357515732608 37.0 35.0 37.0 33.0 37.0 84-85 35.14133485311443 37.0 33.0 37.0 33.0 37.0 86-87 35.050262928516005 37.0 33.0 37.0 33.0 37.0 88-89 34.94836735280313 37.0 33.0 37.0 33.0 37.0 90-91 34.84270744533099 37.0 33.0 37.0 33.0 37.0 92-93 34.77574927443224 37.0 33.0 37.0 33.0 37.0 94-95 34.73672187048017 37.0 33.0 37.0 33.0 37.0 96-97 34.63926111818852 37.0 33.0 37.0 33.0 37.0 98-99 34.57946762962041 37.0 33.0 37.0 33.0 37.0 100-101 33.80286108370609 35.0 33.0 37.0 30.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1391834480098879 2306 2 0.11172353916587241 2306 3 -0.028425291376422024 2306 4 0.09416063217443593 2306 5 0.04290945645347932 2306 6 0.1317901810086255 2306 7 0.17431627204541655 2306 8 -0.009209801300933407 2306 9 0.1250123005543884 2306 10-11 -0.13003084395131737 2306 12-13 -0.10558493453833506 2306 14-15 0.07778712523205655 2306 16-17 0.130874081506839 2306 18-19 0.24426101777827114 2306 20-21 -0.2916878341255398 2306 22-23 0.19901075133734736 2306 24-25 0.24993368255606896 2306 26-27 0.6063083230385473 2306 28-29 0.4239460060352158 2306 30-31 0.2879799075732947 2306 32-33 -0.05339452279329748 2306 34-35 0.1128183851208675 2306 36-37 0.38324421518803575 2306 38-39 0.6171666275807013 2306 40-41 0.2297828123003356 2306 42-43 0.10203155563272759 2306 44-45 0.459236236337766 2306 46-47 0.3485185130171331 2306 48-49 0.6407447932834103 2306 50-51 0.3288077748728071 2306 52-53 0.36094131582782296 2306 54-55 0.5062635841967875 2306 56-57 0.582206768131833 2306 58-59 0.4164313594171176 2306 60-61 0.3534873525222153 2306 62-63 0.34524577023071856 2306 64-65 -0.11578963206981996 2306 66-67 -0.16886967438871636 2306 68-69 -0.15993548669690227 2306 70-71 -0.10803102126251929 2306 72-73 0.3158296672633156 2306 74-75 0.24046660239843476 2306 76-77 0.022841353907601558 2306 78-79 0.47899752049866606 2306 80-81 0.7146747640413267 2306 82-83 0.713662021460955 2306 84-85 0.5070849416589809 2306 86-87 0.7378185187496982 2306 88-89 1.0021649674052782 2306 90-91 0.8229618504799063 2306 92-93 0.6714015162535603 2306 94-95 -0.14488856050778054 2306 96-97 0.2950508758748711 2306 98-99 0.16053074704061743 2306 100-101 0.4289802541254417 2307 1 0.13770178360687169 2307 2 -0.1594210465854573 2307 3 0.09097216509968575 2307 4 0.15910692183982889 2307 5 0.21774585600898178 2307 6 0.12833296406826378 2307 7 0.1713529432393912 2307 8 0.09623531538013452 2307 9 0.09957706163600477 2307 10-11 -0.05514505558237914 2307 12-13 -0.013598269517963502 2307 14-15 0.09272724129576204 2307 16-17 0.29749959082897703 2307 18-19 0.3283454726492394 2307 20-21 0.2068922374882476 2307 22-23 0.21271614706520836 2307 24-25 0.49484046117071756 2307 26-27 0.1330400183095719 2307 28-29 0.13063819025548185 2307 30-31 0.2829792902131274 2307 32-33 0.22349151251970767 2307 34-35 0.11553476985972111 2307 36-37 0.16618038014667036 2307 38-39 0.4537513911317532 2307 40-41 0.11804062190646647 2307 42-43 0.37336134943443255 2307 44-45 0.40330340512404206 2307 46-47 0.2096124752346995 2307 48-49 -0.008718103370490837 2307 50-51 0.20471838112049312 2307 52-53 0.31538013543517707 2307 54-55 0.17918616723172676 2307 56-57 0.2742675163723547 2307 58-59 0.4164313594171176 2307 60-61 0.4317068857979294 2307 62-63 0.5642234217926401 2307 64-65 0.17251756634973958 2307 66-67 -0.08460001146736573 2307 68-69 0.2851811943748359 2307 70-71 0.2699786095561052 2307 72-73 -0.06699537286509383 2307 74-75 0.5153770851740873 2307 76-77 0.1878617267931375 2307 78-79 0.034189519510889 2307 80-81 0.48254734090266993 2307 82-83 0.6697059775049112 2307 84-85 0.057338059327825874 2307 86-87 0.42000150430346395 2307 88-89 0.513647866528629 2307 90-91 0.5296540347001795 2307 92-93 0.40698615633258584 2307 94-95 0.5835347016233428 2307 96-97 0.306533774998222 2307 98-99 0.07829837267340878 2307 100-101 0.15413150736657855 2201 1 -0.16861985979342364 2201 2 -0.004160576718241771 2201 3 0.09778183483070535 2201 4 0.12324266125646233 2201 5 0.08087149441551844 2201 6 0.16442421364265414 2201 7 0.20517430290344407 2201 8 -0.18636597845711123 2201 9 0.17607235161443668 2201 10-11 0.11583440191392924 2201 12-13 0.018180189226782772 2201 14-15 -0.19815915071422552 2201 16-17 0.021594472227228323 2201 18-19 0.15401792753518606 2201 20-21 0.24372120128349906 2201 22-23 0.05881761114419959 2201 24-25 -0.03983210720971897 2201 26-27 -0.08072686399663809 2201 28-29 -0.1778965958073897 2201 30-31 0.1948508144442016 2201 32-33 0.07214660274783569 2201 34-35 0.09799987030235258 2201 36-37 0.3304636624074817 2201 38-39 0.3815688919829654 2201 40-41 -0.15122506870753938 2201 42-43 -0.030225076623899838 2201 44-45 -0.03660125949972581 2201 46-47 -0.2125870480884231 2201 48-49 -0.16522701268839057 2201 50-51 -0.02800208193704634 2201 52-53 0.3682118230983278 2201 54-55 0.06625914419234391 2201 56-57 -0.04277935685429668 2201 58-59 -0.19973025674450184 2201 60-61 0.031809030843895414 2201 62-63 0.16387158885653008 2201 64-65 0.23130771502753333 2201 66-67 0.20991820439915898 2201 68-69 -0.10399143075284911 2201 70-71 -0.22996464319614063 2201 72-73 -0.7570419056082542 2201 74-75 -0.39456503263320286 2201 76-77 -0.17157534050910073 2201 78-79 0.04820608970723583 2201 80-81 -0.43545288608632404 2201 82-83 -0.022213214414279037 2201 84-85 -0.0726131380390953 2201 86-87 0.13320091413208957 2201 88-89 -0.40781394257362535 2201 90-91 -0.5869615594435018 2201 92-93 -0.2666604218083748 2201 94-95 -0.4258853415045678 2201 96-97 -0.1149280341040324 2201 98-99 0.0067400932499666055 2201 100-101 -0.0725737474285637 1101 1 0.1399001226396024 1101 2 0.11244021379558689 1101 3 0.027953112828072335 1101 4 0.051876829021068716 1101 5 0.05616793710326107 1101 6 0.1986798131156462 1101 7 -0.19381559608645205 1101 8 -0.1254305370868849 1101 9 0.12644564981382445 1101 10-11 0.04842113884848942 1101 12-13 0.11622586335941065 1101 14-15 -0.05987412655963453 1101 16-17 -0.19700456158920332 1101 18-19 0.3448940803678582 1101 20-21 0.28780332688736365 1101 22-23 0.1325989023167935 1101 24-25 -0.27341796579558064 1101 26-27 -0.06694776869580465 1101 28-29 -0.24124749611481633 1101 30-31 -0.22318827207314484 1101 32-33 -0.19547526813491345 1101 34-35 -0.13849551836695184 1101 36-37 -0.4356759950365259 1101 38-39 -0.21955100261519078 1101 40-41 -0.6346462369113226 1101 42-43 -0.22757905115179256 1101 44-45 -0.7908832094338507 1101 46-47 -0.3940758491424461 1101 48-49 -0.664678044748122 1101 50-51 -0.4589968118448269 1101 52-53 -0.682238330612698 1101 54-55 -0.19422614346685663 1101 56-57 -0.5786269633540755 1101 58-59 -0.40422081449592895 1101 60-61 -0.7414123130296204 1101 62-63 -0.6927379851443405 1101 64-65 -0.5595903965227578 1101 66-67 -0.5601740222148024 1101 68-69 -0.5491495335196461 1101 70-71 -0.28815524486500266 1101 72-73 -0.4256299600658764 1101 74-75 -0.4658759680745632 1101 76-77 -0.3042608916729108 1101 78-79 -0.2487855659800715 1101 80-81 -0.3252201236796495 1101 82-83 -0.3629027181472608 1101 84-85 -0.22917879460475632 1101 86-87 -0.16280259926272578 1101 88-89 -0.1555631740185106 1101 90-91 -0.4183186081918535 1101 92-93 -0.3827671411759681 1101 94-95 0.23918931813531685 1101 96-97 -0.1561555264184804 1101 98-99 0.015523580294320993 1101 100-101 0.6054016588077147 2304 1 0.08390539273182895 2304 2 0.11705154449387578 2304 3 0.15838789543676768 2304 4 -0.018171480157676 2304 5 0.020265433809456113 2304 6 0.14422219344064047 2304 7 0.22537632310546485 2304 8 0.056058263967130983 2304 9 -0.04614987060778475 2304 10-11 0.11583440191392924 2304 12-13 0.06363473468132952 2304 14-15 0.06951761696254977 2304 16-17 0.39533184596459847 2304 18-19 0.33078560430286075 2304 20-21 0.3043272618895614 2304 22-23 0.08407013639672556 2304 24-25 0.35410728672967196 2304 26-27 0.5202832370134587 2304 28-29 0.28674986883907394 2304 30-31 0.5685881881815789 2304 32-33 0.6933587239599532 2304 34-35 0.5676968399993214 2304 36-37 0.6738980058418278 2304 38-39 0.6239931344072076 2304 40-41 0.1972597797773048 2304 42-43 0.12634057994175407 2304 44-45 0.67046944757098 2304 46-47 0.33791800241662884 2304 48-49 0.430732583271201 2304 50-51 0.6083615544265868 2304 52-53 0.32780778269428623 2304 54-55 0.5107035886367868 2304 56-57 0.17439236031741956 2304 58-59 0.04269398567974747 2304 60-61 0.309586808621674 2304 62-63 0.5376089625939073 2304 64-65 0.6353481190679346 2304 66-67 0.5634535579345155 2304 68-69 0.633382306620895 2304 70-71 0.6437727305412366 2304 72-73 0.34396819540184254 2304 74-75 0.7670511289829633 2304 76-77 0.33347516454140447 2304 78-79 0.13911518061632933 2304 80-81 0.3726279219944786 2304 82-83 0.5131403209392502 2304 84-85 0.594053528627569 2304 86-87 0.3756251565563318 2304 88-89 0.34976181500212533 2304 90-91 0.6554626829807404 2304 92-93 0.253541598393646 2304 94-95 -0.40568332130254703 2304 96-97 0.2184052992292962 2304 98-99 0.703709790219655 2304 100-101 0.3264161515613324 2202 1 0.08747045512042462 2202 2 0.060010546276409116 2202 3 0.040740836613238685 2202 4 -0.031243375582512556 2202 5 -0.30593777474669537 2202 6 0.05034221720772081 2202 7 -0.10558030196880708 2202 8 -0.6819096504713684 2202 9 -0.2921391754206155 2202 10-11 -0.05558568127086261 2202 12-13 -0.12056015539591414 2202 14-15 -0.4939107847011499 2202 16-17 -0.24474539705381915 2202 18-19 -0.222987420058395 2202 20-21 -0.20399121701715472 2202 22-23 -0.25891857423904696 2202 24-25 -0.11900620108969662 2202 26-27 -0.20089917534541968 2202 28-29 -0.43071893686502705 2202 30-31 -0.4551788944090376 2202 32-33 -0.7816858393199055 2202 34-35 -0.2580607357582565 2202 36-37 -1.1534935568438627 2202 38-39 -0.23830633083343145 2202 40-41 -0.04278775438788074 2202 42-43 -0.03586975873917453 2202 44-45 -0.36057630406300945 2202 46-47 -0.5682020213504515 2202 48-49 -0.16403865855885869 2202 50-51 -0.031567144325642005 2202 52-53 -0.4547234116016128 2202 54-55 -0.49761489026992933 2202 56-57 -0.4792024109244082 2202 58-59 -0.6669019739162181 2202 60-61 -0.6174779566783783 2202 62-63 -0.6492597242747777 2202 64-65 -0.5827148637009358 2202 66-67 -0.3453402626240063 2202 68-69 -0.8009611277225446 2202 70-71 -1.2480870436714753 2202 72-73 -0.8249266352576896 2202 74-75 -0.5175596850396218 2202 76-77 -0.06566327871468758 2202 78-79 -0.34469349436881913 2202 80-81 -0.009576474915796496 2202 82-83 0.30205891868138224 2202 84-85 0.050232970101127705 2202 86-87 -0.354618456040221 2202 88-89 -0.5823534553484393 2202 90-91 -0.7326834845771941 2202 92-93 -0.5474090849099795 2202 94-95 -0.5744296076958904 2202 96-97 -0.7502518606043296 2202 98-99 -0.9448344759716676 2202 100-101 -0.35985835824258317 1102 1 -0.11120901666829042 1102 2 -0.0060158642878178625 1102 3 0.09716340564084902 1102 4 0.16323441553393536 1102 5 0.11983253337655242 1102 6 0.18359551852825007 1102 7 0.2653680773829379 1102 8 0.1556253635342273 1102 9 0.13443145283068247 1102 10-11 0.22632708383518008 1102 12-13 0.30100847205506653 1102 14-15 0.5047886950907667 1102 16-17 0.5935899370798339 1102 18-19 0.5280129801016571 1102 20-21 0.35823367293882313 1102 22-23 0.5483043149166136 1102 24-25 0.5250514219595246 1102 26-27 0.294298903886272 1102 28-29 0.4098172776207676 1102 30-31 0.34724207397831464 1102 32-33 0.7629320078189465 1102 34-35 0.3796943662825569 1102 36-37 0.25687058881440095 1102 38-39 0.6340426087423978 1102 40-41 0.9012898766645421 1102 42-43 0.4360189967630248 1102 44-45 0.7900839600426366 1102 46-47 0.8461637249480631 1102 48-49 0.5571498168312914 1102 50-51 0.6109898784834797 1102 52-53 0.5006587412595351 1102 54-55 0.5459025166928626 1102 56-57 0.6579524510203711 1102 58-59 0.5592885022742635 1102 60-61 0.5870038360387042 1102 62-63 0.677270887970117 1102 64-65 0.3861726913210788 1102 66-67 0.3719466521419008 1102 68-69 0.6242089403046691 1102 70-71 0.8001322440436098 1102 72-73 1.045421503998007 1102 74-75 0.7212358331676683 1102 76-77 0.8529041482561084 1102 78-79 0.8749943807812457 1102 80-81 0.8810797875892007 1102 82-83 0.9911345489334806 1102 84-85 0.7912293686605452 1102 86-87 0.9623083146680571 1102 88-89 0.7564820788652469 1102 90-91 0.8241392445144413 1102 92-93 0.8256401347778919 1102 94-95 0.9402762746570517 1102 96-97 1.0595720689674906 1102 98-99 1.1412999778098438 1102 100-101 0.8332188726497733 2305 1 0.14571157334989238 2305 2 0.058845723911822745 2305 3 0.09898195484270644 2305 4 0.10504084107444811 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60-61 -0.7522818782470111 1213 62-63 -0.5659263909414491 1213 64-65 -0.7579599617401485 1213 66-67 -0.5438696743887164 1213 68-69 -0.11693334994476601 1213 70-71 -0.5370984815799744 1213 72-73 -0.42623382480017824 1213 74-75 -0.11457765889582561 1213 76-77 -0.9594541283878826 1213 78-79 -0.9978797688786187 1213 80-81 -0.9660715729550162 1213 82-83 -0.7330718002728673 1213 84-85 -3.256956572382535 1213 86-87 -1.595018782837606 1213 88-89 -2.672018488028172 1213 90-91 -3.242895902877848 1213 92-93 -2.190587189485143 1213 94-95 -1.7263903920096197 1213 96-97 -2.5596881351141363 1213 98-99 -1.4127674825076113 1213 100-101 -1.614556070660889 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 2.0 15 2.0 16 11.0 17 16.0 18 27.0 19 47.0 20 60.0 21 78.0 22 101.0 23 143.0 24 207.0 25 244.0 26 322.0 27 454.0 28 547.0 29 731.0 30 906.0 31 1242.0 32 1737.0 33 2403.0 34 3590.0 35 5886.0 36 13393.0 37 38726.0 38 33466.0 39 60.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.87633213217818 23.43881775665108 10.932109177336502 24.752740933834243 2 14.936153499822783 21.949210180954296 41.06580069163051 22.048835627592418 3 18.224836200620715 26.241426874592893 32.32978275029695 23.203954174489443 4 12.749877874733 16.681832549496654 42.571431308129235 27.996858267641116 5 11.493184931178819 37.742933496805584 38.30135726669285 12.462524305322747 6 27.27272727272727 41.814733575348896 17.9749236118428 12.937615540081035 7 24.021800557465927 32.96903286366989 24.96144672943746 18.04771984942673 8 21.831208513328416 39.29176923592686 22.43465100908995 16.442371241654776 9 22.448060842329095 16.232603135985286 24.471987816208657 36.84734820547696 10-11 23.161655539697897 26.156358655568436 31.739638509209684 18.94234729552399 12-13 21.521345580981023 24.460493673432246 31.915882031781308 22.102278713805422 14-15 20.651143188283637 26.880968573097956 26.183178322046725 26.28470991657168 16-17 20.1746151856783 32.514535301386 27.71477284700338 19.59607666593232 18-19 19.444258196760565 31.549985153398914 30.187929234394307 18.81782741544621 20-21 20.077393894694495 29.072997385082516 29.959483146713158 20.89012557350983 22-23 19.88295131272689 29.652014827444184 30.299039281232936 20.165994578595992 24-25 20.032854091435905 30.3090966561623 29.98247143226597 19.67557782013582 26-27 19.828833057154625 30.131895288359306 29.88381337343512 20.155458281050947 28-29 19.678451355829925 30.237737186425417 30.21810135918238 19.865710098562275 30-31 19.943774484918727 29.993965575042385 30.374230131895285 19.6880298081436 32-33 19.75316328387659 30.106512389728067 29.968582676411142 20.171741649984195 34-35 19.757473587417746 29.80958036800414 30.348368310648365 20.084577733929752 36-37 19.837453664236932 30.28275591229969 30.272219614754647 19.60757080870873 38-39 19.71006024846505 29.57155582800931 30.132853133590675 20.58553078993496 40-41 19.989272133408683 29.622800547887472 30.235821495962682 20.152105822741163 42-43 20.3633106962577 29.67548203561269 29.92404287315256 20.037164394977058 44-45 19.614754647943986 29.513606191511577 30.296165745538833 20.575473415005604 46-47 19.567341308991292 29.207574640089657 31.007365829829215 20.217718221089836 48-49 19.32261185237689 29.280370877673583 30.82537523586939 20.571642034080135 50-51 19.27615635865557 29.252593365963925 30.60650760050191 20.864742674878592 52-53 18.971082652465014 29.492054673805807 31.087345906648405 20.449516767080773 54-55 18.848957385465656 29.423568739763027 31.182172584553786 20.545301290217527 56-57 19.00604400340993 29.197517265160293 30.863210122508406 20.933228608921368 58-59 18.360456317468223 29.450388406241316 31.28993017308263 20.899225103207826 60-61 19.010833229566767 29.678355571306785 30.376145822358026 20.93466537676842 62-63 18.708633059070316 28.509784389038415 30.793766343234257 21.987816208657005 64-65 18.53430522696143 28.745893238570513 30.714265189030755 22.005536345437303 66-67 18.871945671018477 28.739188321950937 30.03802645568529 22.350839551345295 68-69 19.08985546115459 28.842635606938632 29.585923506479823 22.48158542542696 70-71 19.16169385350715 28.95230888593021 29.453740864551104 22.43225639601153 72-73 18.75508855279164 29.351730347410466 29.511690501048843 22.381490598749053 74-75 19.307765251290697 28.58018601354393 28.823957624926965 23.28809111023841 76-77 19.2751985134242 29.39004415666517 28.746372161186194 22.588385168724436 78-79 19.344642292698346 29.883334450819437 28.19130084960872 22.580722406873495 80-81 19.446173887223303 29.50019635827243 28.183159165142097 22.87047058936217 82-83 19.09656037777416 29.70517523778508 28.39292727081158 22.80533711362918 84-85 19.311596632216165 29.80718575492572 28.17214394498137 22.709073667876744 86-87 19.457668029999713 30.157278186990546 27.853181482935984 22.531872300073754 88-89 19.670788593978987 29.29138609783431 28.160170879589273 22.877654428597427 90-91 19.789082480052873 29.14579362266645 28.20423176023218 22.8608921370485 92-93 19.452878803842875 28.63765672742598 28.29235352151799 23.61711094721315 94-95 19.59416097546958 28.675491614065002 28.481049032097395 23.249298378368024 96-97 20.42461279106522 29.10508520033333 27.56439114567868 22.905910862922767 98-99 19.681324891524028 28.570128638614573 28.282296146588635 23.466250323272767 100-101 19.755557896955008 28.284690759667054 28.656813632053336 23.302937711324603 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 1.0 10 1.5 11 1.0 12 2.5 13 6.0 14 9.0 15 16.5 16 29.5 17 42.5 18 63.5 19 91.5 20 151.0 21 239.5 22 348.5 23 455.0 24 619.5 25 844.5 26 1047.5 27 1264.5 28 1507.5 29 1833.5 30 2193.0 31 2521.5 32 2786.0 33 2993.5 34 3213.0 35 3460.0 36 3737.0 37 3889.0 38 4099.0 39 4289.5 40 4410.0 41 4461.0 42 4463.0 43 4568.0 44 4540.5 45 4422.5 46 4246.0 47 4061.5 48 3796.5 49 3496.5 50 3233.0 51 2973.5 52 2597.0 53 2188.0 54 1885.5 55 1557.5 56 1258.5 57 1024.0 58 833.5 59 660.0 60 510.0 61 404.0 62 310.5 63 212.0 64 146.5 65 106.0 66 74.5 67 57.0 68 44.5 69 29.5 70 20.5 71 15.5 72 12.0 73 6.0 74 4.0 75 4.0 76 3.0 77 2.0 78 1.0 79 2.0 80 1.5 81 0.5 82 0.5 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05459717818794839 2 0.009578452313675156 3 0.004789226156837578 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 104401.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.01829000133452 #Duplication Level Percentage of deduplicated Percentage of total 1 97.86582176474215 93.96908855425929 2 1.410349175219984 2.7083863221883075 3 0.37238354167701554 1.0726689268940317 4 0.13059158965499157 0.5015672450891303 5 0.09644299832239218 0.4630145890758836 6 0.03388976897617989 0.19524225993798422 7 0.025499137354078032 0.17138684956734085 8 0.021031231839341764 0.1615506334268174 9 0.00774937452309194 0.06696735212584758 >10 0.033234265712319466 0.4804069183543941 >50 0.003007151978444763 0.2097203490809668 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10-11 0.0 0.0 0.0 12-13 0.0 0.0 0.0 14-15 0.0 0.0 0.0 16-17 0.0 0.0 0.0 18-19 0.0 0.0 9.578452313675157E-4 20-21 4.7892261568375783E-4 0.0 0.0019156904627350313 22-23 9.578452313675157E-4 0.0 0.002873535694102547 24-25 9.578452313675157E-4 0.0 0.0038313809254700627 26-27 9.578452313675157E-4 0.0 0.007662761850940125 28-29 9.578452313675157E-4 0.0 0.03591919617628184 30-31 9.578452313675157E-4 0.0 0.06225994003888852 32-33 9.578452313675157E-4 0.0 0.09147421959559775 34-35 9.578452313675157E-4 0.0 0.15612877271290504 36-37 9.578452313675157E-4 0.0 0.32231492035516907 38-39 0.0019156904627350313 0.0 0.729878066302047 40-41 0.0019156904627350313 0.0 1.2523826400130267 42-43 0.0019156904627350313 0.0 1.852472677464775 44-45 0.0019156904627350313 0.0 2.675261731209471 46-47 0.0019156904627350313 0.0 3.699677206157029 48-49 0.0019156904627350313 0.0 4.599093878411127 50-51 0.0019156904627350313 0.0 5.524372371912147 52-53 0.0019156904627350313 0.0 6.372065401672398 54-55 0.0019156904627350313 0.0 7.315542954569401 56-57 0.0019156904627350313 0.0 8.382582542312814 58-59 0.0019156904627350313 0.0 9.392630338789857 60-61 0.0019156904627350313 0.0 10.361011867702416 62-63 0.0019156904627350313 0.0 11.293474200438693 64-65 0.0019156904627350313 0.0 12.253235122268944 66-67 0.0019156904627350313 0.0 13.277650597216502 68-69 0.0019156904627350313 9.578452313675157E-4 14.318349441097308 70-71 0.0019156904627350313 9.578452313675157E-4 15.290562350935335 72-73 0.0019156904627350313 9.578452313675157E-4 16.26900125477725 74-75 0.0019156904627350313 9.578452313675157E-4 17.21056311721152 76-77 0.0019156904627350313 9.578452313675157E-4 18.15882989626536 78-79 0.0019156904627350313 9.578452313675157E-4 19.07452993745271 80-81 0.0019156904627350313 9.578452313675157E-4 19.97586230016954 82-83 0.0019156904627350313 9.578452313675157E-4 20.84031762147872 84-85 0.0019156904627350313 9.578452313675157E-4 21.664064520454787 86-87 0.0019156904627350313 9.578452313675157E-4 22.535224758383542 88-89 0.0019156904627350313 9.578452313675157E-4 23.45667187095909 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTACGTT 25 0.0050086314 56.999714 4 ACACCAT 55 4.9323822E-5 43.1816 6 TAGACAG 50 0.0016091339 37.99981 5 CAGGAGA 50 0.0016091339 37.99981 4 TTGGCCT 55 0.0025690056 34.54528 4 AACAGTG 55 0.0025690056 34.54528 7 AGCAGTG 55 0.0025690056 34.54528 2 CACCATG 55 0.0025690056 34.54528 7 AAGCAGT 60 0.0039255335 31.68168 1 CAGTTCT 65 0.005820228 29.23062 9 CTACACT 65 0.005820228 29.23062 4 AACACCA 90 8.893211E-4 26.388756 5 CAACACC 100 0.0016436783 23.74988 4 ACATTTC 125 0.005995465 18.999905 8 ATACTTT 125 0.005995465 18.999905 6 GTAGGAT 85 0.0017810215 16.764622 36-37 TGTGAAA 110 5.6733546E-4 15.11356 74-75 GTCTTCT 220 1.6334525E-9 15.11356 94-95 TAGGATT 100 0.0052590547 14.249928 36-37 GTACTGT 240 0.0010900776 13.854096 9 >>END_MODULE