FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L182_AGGCAGAA-CTCTCTAT_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L182_AGGCAGAA-CTCTCTAT_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104401
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGTT250.005008631456.9997144
ACACCAT554.9323822E-543.18166
TAGACAG500.001609133937.999815
CAGGAGA500.001609133937.999814
TTGGCCT550.002569005634.545284
AACAGTG550.002569005634.545287
AGCAGTG550.002569005634.545282
CACCATG550.002569005634.545287
AAGCAGT600.003925533531.681681
CAGTTCT650.00582022829.230629
CTACACT650.00582022829.230624
AACACCA908.893211E-426.3887565
CAACACC1000.001643678323.749884
ACATTTC1250.00599546518.9999058
ATACTTT1250.00599546518.9999056
GTAGGAT850.001781021516.76462236-37
TGTGAAA1105.6733546E-415.1135674-75
GTCTTCT2201.6334525E-915.1135694-95
TAGGATT1000.005259054714.24992836-37
GTACTGT2400.001090077613.8540969
Produced by FastQC (version 0.11.2)