FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L176_TAAGGCGA-CTCTCTAT_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L176_TAAGGCGA-CTCTCTAT_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4007778
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT15700.024.4986134
TATACCG8700.019.648955
GTGCTAG7900.019.2332151
TACACTG23050.019.158795
TACACAG16100.018.876015
CTACACT20150.018.852594
ACCACGT18950.018.29518
ATAGGAC7050.018.1855053
TATACAG18150.018.0521355
GGTCGCC29350.018.0518294-95
CTGTCCG7900.018.034299
ACTGTGC15150.017.8683058
TCTATAC12850.017.7373543
TAGACAG13450.017.2993565
GTGTAAG14300.017.2661821
GCTGTCC9700.017.1363168
CTACACA19400.017.133754
CCTACAC16850.016.908383
TACACCG10450.016.8128575
GTCGCCG25300.016.6217694-95
Produced by FastQC (version 0.11.2)