FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L176_TAAGGCGA-CTCTCTAT_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L176_TAAGGCGA-CTCTCTAT_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4007778
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG23300.021.201455
TATACCG8650.019.7685365
CTATACT16250.019.5843684
CTAGTAC9950.019.0976183
TACACCG11050.018.9137885
ACCGTGC7000.018.3211988
TACACAG16300.017.775855
GTACACT17950.016.935724
CTACACT20750.016.7106344
GCTACAC10550.016.6606373
GTGTATA16600.016.6098541
AGTGTCC14050.016.5656268
GTGTAGA27850.016.557411
GCTGTCC11800.016.5040288
ACCGCGT4901.8189894E-1216.4793838
GTATAGA14600.016.2803231
CCTACAC17850.016.2343143
ACACTGC17700.016.1014926
ACCACGT18350.016.0488458
GTGTACG9800.016.0078771
Produced by FastQC (version 0.11.2)