FastQCFastQC Report
Thu 11 Sep 2014
lane5_Undetermined_L005_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane5_Undetermined_L005_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3023289
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTCC7700.014.1207963
CATTCCT8953.8926373E-1010.0358144
TCGGAAG20050.09.4312144
AGATCGG20300.09.3152241
GATCGGA20400.09.0169292
ATCGGAA23350.08.7056983
CGTATCA4801.1874181E-78.41655850-51
TGTAGGG11300.08.40488718-19
TGAACCG5957.177732E-97.97291612-13
GCCGTAT5153.6687015E-77.84462548-49
GTGTAGG11400.07.70298616-17
CGGAAGA25600.07.3865575
GTAGGGA11400.07.289765418-19
GAGTGTA6056.2231084E-77.06907728-29
TAGGGAA13300.06.965665320-21
AGTGTAG5802.4168803E-66.96412428-29
TCGTGTA12100.06.860816514-15
AGATCTC9701.3460522E-106.61455234-35
GTATCAT5302.6232994E-46.27738850-51
CCGTATC6501.42039335E-56.215357348-49
Produced by FastQC (version 0.11.2)