FastQCFastQC Report
Thu 11 Sep 2014
lane5_Undetermined_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane5_Undetermined_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3023289
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT15800.016.60038412-13
AGAGCAC21100.015.4480228
CGTCTGA16400.015.30708916-17
CTCCAGT17550.014.136765524-25
AGCACAC18900.014.12364310-11
CACGTCT17200.013.89826914-15
CCAGTCA15900.013.81626926-27
TCGGAAG25450.013.5511843
GATCGGA25650.013.2687981
TGAACTC20750.012.43578920-21
CGGAAGA28250.012.3742684
AACTCCA21600.012.26271722-23
ATCGGAA28700.012.0170392
GCACACG16550.010.27695810-11
ACACGTC16600.09.96419512-13
AGATCGG21800.09.7575871
GAGCACA36900.09.7295669
GAACTCC18700.09.6213720-21
ACGTCTG16900.09.523355514-15
TAGTATG2250.00113775079.47856718-19
Produced by FastQC (version 0.11.2)