FastQCFastQC Report
Thu 11 Sep 2014
QG2083_GTGAAA_L005_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameQG2083_GTGAAA_L005_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61660725
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGT331950.08.9548929
GTGTAGG195550.08.01342116-17
GTAGGGA185500.07.961447718-19
TGTAGGG191350.07.730457318-19
CGTGTAG197500.07.50152116-17
AAGAGCG428650.07.13413578
TCGTGTA223800.06.991018314-15
AGATCGG469200.06.77958151
GATCGGA570950.05.63774872
AGCGTCG313850.05.53762810-11
GTCGTGT310950.05.352326414-15
GCGTCGT310100.05.229501712-13
TAGGGAA307100.05.141807620-21
GAGCGTC314250.05.130141310-11
TCGGAAG672200.05.01484544
CGTCGTG339300.05.010378412-13
Produced by FastQC (version 0.11.2)