FastQCFastQC Report
Thu 11 Sep 2014
QG2083_GTGAAA_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameQG2083_GTGAAA_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61660725
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC202850.014.83417846-47
TATGCCG235550.012.85543348-49
CTCGTAT243400.011.81643244-45
AGTCACG261550.011.80433128-29
CACACGT295300.010.42326612-13
GTATGCC185250.08.75637646-47
TGCCGTC391950.08.12563950-51
AGCACAC397700.08.03806210-11
AACGATC378800.07.83097438-39
TCGTATG213300.07.771902644-45
CCAGTCA417250.07.63282926-27
CGATCTC405550.07.46672240-41
CGTCTGA378900.07.446840316-17
ATCTCGT425400.07.2411342-43
AGAGCAC484100.07.1429628
TCACGTG460100.07.08713530-31
CTCCAGT471450.07.012239524-25
CCGTCTT469200.06.89402652-53
AACTCCA490150.06.880391622-23
TGCTTGA468700.06.87601760-61
Produced by FastQC (version 0.11.2)