FastQCFastQC Report
Thu 11 Sep 2014
QG2080_CTTGTA_L005_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameQG2080_CTTGTA_L005_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26386356
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGT230750.033.3173839
AAGAGCG270300.028.8112378
AGATCGG302150.025.98981
GATCGGA333050.023.6494582
TCGGAAG365400.021.6867794
GTAGGGA175000.020.5960518-19
CGTGTAG182700.020.07822416-17
TGTAGGG184600.020.06518218-19
TCGTGTA188200.019.78041814-15
GTGTAGG192400.019.73234716-17
CGGAAGA406800.019.643125
GAAGAGC421850.018.8538597
GCCGTAT87100.018.51533348-49
GTCGTGT207700.018.09478414-15
GAGCGTC217900.017.83769610-11
ATCGGAA470950.017.2094123
AGCGTCG232800.017.17538310-11
CGTCGTG237400.016.47291612-13
GCGTCGT235000.016.43907212-13
TAGGGAA220300.016.3835720-21
Produced by FastQC (version 0.11.2)