FastQCFastQC Report
Thu 11 Sep 2014
QG2080_CTTGTA_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameQG2080_CTTGTA_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26386356
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG630390.23890756268125846TruSeq Adapter, Index 12 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC310700.11775024940920224TruSeq Adapter, Index 12 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC190750.030.40466944-45
TATGCCG196000.029.55387746-47
CTCGTAT209050.027.22053342-43
AGATCGG306750.026.1846791
CACACGT245200.024.0890812-13
AGTCACC244800.023.7395628-29
TAATCTC246000.023.12230938-39
AGAGCAC341300.023.0604159
TGCCGTC257250.022.53555548-49
GATCGGA371000.021.8951342
CTTGTAA270900.021.81196434-35
TGTAATC268350.021.27609836-37
CGTCTGA241700.021.02953116-17
ATCTCGT277900.020.69031140-41
CCGTCTT280500.020.68456850-51
AGCACAC289350.020.52015310-11
CCAGTCA291350.020.08520126-27
GTATGCC196200.020.00933346-47
TCGTATG196400.020.00106844-45
ACCTTGT296850.019.84905232-33
Produced by FastQC (version 0.11.2)