##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename QG2077_GCCAAT_L005_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 37040089 Sequences flagged as poor quality 0 Sequence length 101 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.747495936092378 33.0 33.0 33.0 27.0 33.0 2 31.828009025572264 33.0 33.0 33.0 27.0 33.0 3 31.852370090147463 33.0 33.0 33.0 27.0 33.0 4 35.64360695785585 37.0 37.0 37.0 33.0 37.0 5 35.64412698900372 37.0 37.0 37.0 33.0 37.0 6 35.60363016406359 37.0 37.0 37.0 33.0 37.0 7 35.584767817377546 37.0 37.0 37.0 33.0 37.0 8 35.545301821494 37.0 37.0 37.0 33.0 37.0 9 35.497020296036546 37.0 37.0 37.0 33.0 37.0 10-11 35.44392140904413 37.0 37.0 37.0 33.0 37.0 12-13 35.383887428024266 37.0 37.0 37.0 33.0 37.0 14-15 37.19187155570819 40.0 37.0 40.0 33.0 40.0 16-17 37.086511806167636 40.0 37.0 40.0 33.0 40.0 18-19 36.935790637544095 40.0 37.0 40.0 33.0 40.0 20-21 36.78364655117324 40.0 37.0 40.0 33.0 40.0 22-23 36.6708305425508 40.0 37.0 40.0 33.0 40.0 24-25 36.45953248114496 40.0 37.0 40.0 33.0 40.0 26-27 36.2337745057794 40.0 37.0 40.0 27.0 40.0 28-29 36.00035967786147 38.5 37.0 40.0 27.0 40.0 30-31 35.743929975438235 37.0 37.0 40.0 27.0 40.0 32-33 35.466668587108416 37.0 37.0 40.0 27.0 40.0 34-35 35.35576261709306 37.0 37.0 40.0 27.0 40.0 36-37 35.465089122761015 37.0 37.0 40.0 27.0 40.0 38-39 35.279900852830025 37.0 37.0 40.0 27.0 40.0 40-41 34.997261507659985 37.0 33.0 40.0 27.0 40.0 42-43 34.67814841103649 37.0 33.0 40.0 24.5 40.0 44-45 34.36852693577491 37.0 33.0 40.0 22.0 40.0 46-47 34.07397333737508 37.0 33.0 40.0 22.0 40.0 48-49 33.74519565814218 37.0 33.0 38.5 22.0 40.0 50-51 32.57882533705575 35.0 30.0 37.0 22.0 38.5 52-53 32.74475359656938 35.0 33.0 37.0 22.0 38.5 54-55 33.565680039267725 37.0 33.0 37.0 22.0 40.0 56-57 33.50621136466492 37.0 33.0 37.0 22.0 40.0 58-59 33.31406965571816 37.0 33.0 37.0 22.0 40.0 60-61 33.10759797310422 37.0 33.0 37.0 22.0 40.0 62-63 32.88775020222009 37.0 33.0 37.0 22.0 40.0 64-65 32.652476536975925 37.0 33.0 37.0 22.0 40.0 66-67 32.39918097928977 37.0 33.0 37.0 22.0 38.5 68-69 32.190732749049275 37.0 33.0 37.0 18.5 37.0 70-71 31.948568333623605 37.0 33.0 37.0 15.0 37.0 72-73 31.71480300708781 37.0 33.0 37.0 15.0 37.0 74-75 31.496141477953792 35.0 33.0 37.0 15.0 37.0 76-77 31.283185132195552 33.0 30.0 37.0 15.0 37.0 78-79 31.067964186047178 33.0 27.0 37.0 15.0 37.0 80-81 30.849726306003205 33.0 27.0 37.0 15.0 37.0 82-83 30.636426697030885 33.0 27.0 37.0 15.0 37.0 84-85 30.435604825355576 33.0 27.0 37.0 15.0 37.0 86-87 30.234311748009027 33.0 27.0 37.0 15.0 37.0 88-89 30.039141941586585 33.0 27.0 37.0 15.0 37.0 90-91 29.814169641978992 33.0 27.0 37.0 15.0 37.0 92-93 29.615229920208883 33.0 27.0 37.0 15.0 37.0 94-95 29.449622110789207 33.0 27.0 37.0 15.0 37.0 96-97 29.275079198648793 33.0 27.0 37.0 15.0 37.0 98-99 29.052451372349566 33.0 27.0 37.0 15.0 37.0 100-101 28.184589877740304 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.38341992831052707 2306 2 0.38682455215246847 2306 3 0.3897847109647934 2306 4 0.49723600530006706 2306 5 0.4684882834364359 2306 6 0.4479960249632171 2306 7 0.4539807686951036 2306 8 0.42164328747965385 2306 9 0.39609248025605837 2306 10-11 0.3373776442253913 2306 12-13 0.31251461913448253 2306 14-15 0.3796359300383969 2306 16-17 0.32159381575201706 2306 18-19 0.2881410935398492 2306 20-21 0.1981452595043578 2306 22-23 0.16953691474964927 2306 24-25 0.06936749745820947 2306 26-27 -0.05119476771624676 2306 28-29 -0.19411456656789738 2306 30-31 -0.25808052376980584 2306 32-33 -0.3454311568368169 2306 34-35 -0.5210289559348027 2306 36-37 -0.45855325578831696 2306 38-39 -0.46728511058180544 2306 40-41 -0.5942743967961945 2306 42-43 -0.6749585255675541 2306 44-45 -0.7394940305052486 2306 46-47 -0.7940278298045058 2306 48-49 -0.8303125621625966 2306 50-51 -0.7771539697575491 2306 52-53 -0.8162276395080319 2306 54-55 -0.8997306621126739 2306 56-57 -1.0432280729183532 2306 58-59 -1.0280420517345803 2306 60-61 -1.08781702257712 2306 62-63 -1.2897089992786874 2306 64-65 -1.353751543754612 2306 66-67 -1.36562530962118 2306 68-69 -1.4219635754624242 2306 70-71 -1.5950024318493945 2306 72-73 -1.6458721554170985 2306 74-75 -1.7024905297817767 2306 76-77 -1.7803220790433159 2306 78-79 -1.8121134812021218 2306 80-81 -1.8863035455557018 2306 82-83 -1.9828395896691973 2306 84-85 -2.0219389931636833 2306 86-87 -2.2101484534155205 2306 88-89 -2.370555647039982 2306 90-91 -2.5201551737237438 2306 92-93 -2.6155872073873674 2306 94-95 -2.59559891119293 2306 96-97 -2.6780393443402595 2306 98-99 -2.789718889593601 2306 100-101 -2.7114660410010814 2307 1 0.43410405314195444 2307 2 0.41525281636916134 2307 3 0.3742511471119023 2307 4 0.5085229735815986 2307 5 0.4929368763477129 2307 6 0.49485046306827485 2307 7 0.4818133698099558 2307 8 0.4382409773348783 2307 9 0.45611332117160686 2307 10-11 0.39034033256870515 2307 12-13 0.3923606852632915 2307 14-15 0.5594866284877185 2307 16-17 0.5844845426813023 2307 18-19 0.5494132997576813 2307 20-21 0.5531292088407582 2307 22-23 0.4213320521107775 2307 24-25 0.40579523094476855 2307 26-27 0.31979025273738415 2307 28-29 0.3284229049315144 2307 30-31 0.20254085399196242 2307 32-33 0.13296055178228272 2307 34-35 0.16867855099959428 2307 36-37 0.12165881898629038 2307 38-39 0.09727726539925641 2307 40-41 0.08058046219114345 2307 42-43 -0.003517903064157224 2307 44-45 -0.05250115894737917 2307 46-47 -0.1872719550061248 2307 48-49 -0.22580628001172443 2307 50-51 -0.340884036947827 2307 52-53 -0.2795684811237109 2307 54-55 -0.28137605531269116 2307 56-57 -0.2570613186349462 2307 58-59 -0.33665534119315765 2307 60-61 -0.38914051829014085 2307 62-63 -0.37536920604616597 2307 64-65 -0.40221580125248835 2307 66-67 -0.5073594542594257 2307 68-69 -0.5846024872362818 2307 70-71 -0.6686711150089302 2307 72-73 -0.7193421919988516 2307 74-75 -0.7568595624627221 2307 76-77 -0.815085129562668 2307 78-79 -0.8568793764994105 2307 80-81 -0.9475215722334447 2307 82-83 -0.9570974677827309 2307 84-85 -1.041568589597528 2307 86-87 -1.1950284536810862 2307 88-89 -1.3015347254486223 2307 90-91 -1.2341181093253333 2307 92-93 -1.2699691964826556 2307 94-95 -1.3024577482420803 2307 96-97 -1.5048341396842382 2307 98-99 -1.6216390382463253 2307 100-101 -1.6182042264713488 2201 1 0.4310286900275848 2201 2 0.39669107724982666 2201 3 0.34405730283110714 2201 4 0.4687595436986456 2201 5 0.45091714176737696 2201 6 0.45266124456832557 2201 7 0.4651209897214912 2201 8 0.44700561679390205 2201 9 0.4274483065583752 2201 10-11 0.42578685106195735 2201 12-13 0.37673250335300423 2201 14-15 0.49436990329414954 2201 16-17 0.4828704811257509 2201 18-19 0.4709392195251283 2201 20-21 0.4996080491452304 2201 22-23 0.38173882835232575 2201 24-25 0.35997584958508355 2201 26-27 0.3898680863194812 2201 28-29 0.3300067588037763 2201 30-31 0.2840560902020428 2201 32-33 0.22864292013959897 2201 34-35 0.22650128987027074 2201 36-37 0.2861488284430749 2201 38-39 0.2569022587554599 2201 40-41 0.16135501873342406 2201 42-43 0.14724870083119157 2201 44-45 -0.01483285519837807 2201 46-47 0.03846574263866387 2201 48-49 -0.001581045310260265 2201 50-51 -0.08381862359161829 2201 52-53 0.007576609584148741 2201 54-55 0.04385588615420488 2201 56-57 -0.09443926283734783 2201 58-59 -0.046430983560732386 2201 60-61 -0.1404044597966987 2201 62-63 -0.11493864974481482 2201 64-65 -0.17229070112122002 2201 66-67 -0.18071731991678064 2201 68-69 -0.24682412922218333 2201 70-71 -0.31259437867076656 2201 72-73 -0.341518705158137 2201 74-75 -0.4095597333071197 2201 76-77 -0.4235057281811194 2201 78-79 -0.5296798366180901 2201 80-81 -0.5036244289656189 2201 82-83 -0.6307946623817564 2201 84-85 -0.6503517155497285 2201 86-87 -0.7248685151629743 2201 88-89 -0.7802491222848644 2201 90-91 -0.8286561440407105 2201 92-93 -0.8850923932175689 2201 94-95 -0.8290979053048559 2201 96-97 -0.852218583925815 2201 98-99 -0.944535915645158 2201 100-101 -0.996521818568425 1101 1 0.41194119151855446 1101 2 0.38268731151449487 1101 3 0.3829045614441604 1101 4 0.47198710443094427 1101 5 0.47419569775878756 1101 6 0.46496842012448525 1101 7 0.4844626733492632 1101 8 0.48501509879399407 1101 9 0.48013688439248625 1101 10-11 0.46373909366612764 1101 12-13 0.45047481553192625 1101 14-15 0.6179699394978471 1101 16-17 0.5416067860425855 1101 18-19 0.5813899381840244 1101 20-21 0.5834638155737224 1101 22-23 0.4943052731587514 1101 24-25 0.5307055692068801 1101 26-27 0.5239827702200444 1101 28-29 0.4569851169949928 1101 30-31 0.3727999244598408 1101 32-33 0.3403509255548798 1101 34-35 0.38218198908599277 1101 36-37 0.4377928453278841 1101 38-39 0.4144754670152224 1101 40-41 0.348427706179514 1101 42-43 0.34187337553616004 1101 44-45 0.2256575525441633 1101 46-47 0.2383403312295016 1101 48-49 0.31252300846019665 1101 50-51 0.27886272179178917 1101 52-53 0.2896295954885417 1101 54-55 0.3266513574157557 1101 56-57 0.25771587699740195 1101 58-59 0.25993994914176 1101 60-61 0.24182947206929128 1101 62-63 0.2129227868851089 1101 64-65 0.13612098368687242 1101 66-67 0.08648982263784433 1101 68-69 0.17147000068168694 1101 70-71 0.0704967162783845 1101 72-73 0.025536487364313132 1101 74-75 0.0130843805762062 1101 76-77 -0.019988856277734612 1101 78-79 -0.065005470902193 1101 80-81 -0.08671970984735111 1101 82-83 -0.08525941041123275 1101 84-85 -0.03848544794339759 1101 86-87 -0.06293525461951788 1101 88-89 -0.18495013799115156 1101 90-91 -0.19791371664716095 1101 92-93 -0.2585104746175517 1101 94-95 -0.2866968401785819 1101 96-97 -0.34920513402946796 1101 98-99 -0.37156237763872824 1101 100-101 -0.38259699983628437 2304 1 0.15733790034709116 2304 2 0.1564843778034728 2304 3 0.15759182158807405 2304 4 0.23800378751973028 2304 5 0.1401183449359209 2304 6 0.22815733847608755 2304 7 0.21220398599182033 2304 8 0.20180536327289644 2304 9 0.1897017725495047 2304 10-11 0.15293444949417534 2304 12-13 0.15224128605405696 2304 14-15 0.21411668219376168 2304 16-17 0.24494189028947488 2304 18-19 0.2410415025698498 2304 20-21 0.20887547459981448 2304 22-23 0.11783085838535357 2304 24-25 0.19658977611885575 2304 26-27 0.16979499969168899 2304 28-29 0.017319467838213143 2304 30-31 0.0807848956934123 2304 32-33 0.13715069861941487 2304 34-35 0.09544757315390484 2304 36-37 0.13319771250342427 2304 38-39 0.14818529094858235 2304 40-41 0.1616213605949568 2304 42-43 0.10649678391684603 2304 44-45 0.067156131381779 2304 46-47 0.0643006445014862 2304 48-49 0.08213172362737708 2304 50-51 0.03651535836559816 2304 52-53 0.028867972095049765 2304 54-55 0.0068952702011984 2304 56-57 -0.012686495563222877 2304 58-59 0.16202770830738444 2304 60-61 0.11190329044806191 2304 62-63 -0.04574399246585159 2304 64-65 -0.01763555182348142 2304 66-67 -0.027800980605121595 2304 68-69 -0.10310124104618268 2304 70-71 -0.05706820008684588 2304 72-73 -0.08569334575206611 2304 74-75 -0.03419262345659746 2304 76-77 -0.10125889411654754 2304 78-79 -0.23049921854759958 2304 80-81 -0.2713915130074973 2304 82-83 -0.16432522604238287 2304 84-85 -0.23015507627653164 2304 86-87 -0.25209531376015804 2304 88-89 -0.20416547764062187 2304 90-91 -0.2770963035932219 2304 92-93 -0.2784348804515986 2304 94-95 -0.37412762277250877 2304 96-97 -0.33383398115960006 2304 98-99 -0.3345414438354801 2304 100-101 -0.32881609285673363 2202 1 0.19424587218686895 2202 2 0.1770560668334511 2202 3 0.19608809123160498 2202 4 0.22267946244791403 2202 5 0.2817226723448556 2202 6 0.2379064504422388 2202 7 0.30579798538519043 2202 8 0.2940567124469453 2202 9 0.3385865126252483 2202 10-11 0.18796573170904907 2202 12-13 0.27642931436620444 2202 14-15 0.4098398885543588 2202 16-17 0.4086940929998306 2202 18-19 0.4345747629208816 2202 20-21 0.36169370081313446 2202 22-23 0.31637976703445503 2202 24-25 0.40514192329639087 2202 26-27 0.2928305992871998 2202 28-29 0.3868817127443194 2202 30-31 0.40946744657145473 2202 32-33 0.3521033824294335 2202 34-35 0.3840185062339998 2202 36-37 0.4145281511018126 2202 38-39 0.4093614287571157 2202 40-41 0.359920471050593 2202 42-43 0.3187750653629777 2202 44-45 0.34518921374628064 2202 46-47 0.3546946775645381 2202 48-49 0.3236343210778543 2202 50-51 0.32378100452582004 2202 52-53 0.41054045931023353 2202 54-55 0.4633852742874467 2202 56-57 0.4817568096228726 2202 58-59 0.4305727267902384 2202 60-61 0.38795304047503265 2202 62-63 0.4331773697744197 2202 64-65 0.38772968901978544 2202 66-67 0.3624529272056307 2202 68-69 0.3143833559992544 2202 70-71 0.209863859300377 2202 72-73 0.27771499889486506 2202 74-75 0.31901433521181843 2202 76-77 0.23583261496366248 2202 78-79 0.19660193514567936 2202 80-81 0.22719614052972403 2202 82-83 0.23297842434835303 2202 84-85 0.17239010656336617 2202 86-87 0.16732962449746225 2202 88-89 0.11317583916294183 2202 90-91 0.13697945343151474 2202 92-93 0.002175978658065958 2202 94-95 0.005115692421458817 2202 96-97 0.04348762262529249 2202 98-99 0.0689384313056074 2202 100-101 -0.04596781434942443 1102 1 0.34707654380315844 1102 2 0.41106825594393115 1102 3 0.42642061063941483 1102 4 0.4311996924266239 1102 5 0.47398954123534054 1102 6 0.48196470087565757 1102 7 0.49111354942484553 1102 8 0.4747668297466632 1102 9 0.4988417745218712 1102 10-11 0.49124879937944144 1102 12-13 0.47054299084586404 1102 14-15 0.5841811024002865 1102 16-17 0.6530415558635596 1102 18-19 0.6370712357686656 1102 20-21 0.6289436812430296 1102 22-23 0.6286251602603983 1102 24-25 0.6635626933475436 1102 26-27 0.6029065599083552 1102 28-29 0.5784865571153759 1102 30-31 0.6033681763657199 1102 32-33 0.6461346193333739 1102 34-35 0.6406517435671049 1102 36-37 0.6550988063014174 1102 38-39 0.6044793874705263 1102 40-41 0.568543067844189 1102 42-43 0.6094347677403178 1102 44-45 0.6144978337245988 1102 46-47 0.6181198283133256 1102 48-49 0.6191946846276153 1102 50-51 0.6260242355190826 1102 52-53 0.66930124962402 1102 54-55 0.7228485688375699 1102 56-57 0.7479724198133084 1102 58-59 0.7293255171382924 1102 60-61 0.7263525465436089 1102 62-63 0.7073557297746902 1102 64-65 0.6971072935296618 1102 66-67 0.7064453541619855 1102 68-69 0.6776723239345657 1102 70-71 0.6315529219372493 1102 72-73 0.655195403387701 1102 74-75 0.6844112130298008 1102 76-77 0.6673937488559609 1102 78-79 0.6944190082353145 1102 80-81 0.6642880630280779 1102 82-83 0.6872378997401007 1102 84-85 0.6614450500279823 1102 86-87 0.6331156858794316 1102 88-89 0.5216950051321767 1102 90-91 0.6239950016721387 1102 92-93 0.5581093940570554 1102 94-95 0.5876054927995149 1102 96-97 0.4605776001005708 1102 98-99 0.47805233865395635 1102 100-101 0.537422213959367 2305 1 0.347195027614287 2305 2 0.3575559404851276 2305 3 0.3567242471011589 2305 4 0.42767064604034744 2305 5 0.4155060097575429 2305 6 0.4366338917483361 2305 7 0.3769531884207993 2305 8 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46-47 -1.164531925358915 1213 48-49 -1.0587021166796902 1213 50-51 -1.0658004224048838 1213 52-53 -1.1922462022313383 1213 54-55 -1.1716454624928332 1213 56-57 -1.0198221104368272 1213 58-59 -1.0497715267828909 1213 60-61 -0.9985411926859058 1213 62-63 -1.0386530488929857 1213 64-65 -0.8947979472760466 1213 66-67 -0.8842565084978844 1213 68-69 -0.7833788512608777 1213 70-71 -0.7406036044616116 1213 72-73 -0.6862513557885848 1213 74-75 -0.6436902478267612 1213 76-77 -0.5682112261003276 1213 78-79 -0.3769427139290791 1213 80-81 -0.3191669523408045 1213 82-83 -0.22698419784329715 1213 84-85 -0.2439946105146369 1213 86-87 -0.2949248980195591 1213 88-89 -0.15961893332024957 1213 90-91 -0.4087793626800469 1213 92-93 -0.02261108311510185 1213 94-95 0.13697444372025203 1213 96-97 0.11620360921142137 1213 98-99 0.2134396793192863 1213 100-101 0.05809604904137444 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5560.0 3 4217.0 4 4073.0 5 4509.0 6 3588.0 7 12159.0 8 36870.0 9 76698.0 10 102586.0 11 132069.0 12 159112.0 13 173519.0 14 180977.0 15 194456.0 16 213518.0 17 236102.0 18 259726.0 19 283110.0 20 310087.0 21 337520.0 22 368625.0 23 403058.0 24 442837.0 25 488982.0 26 544687.0 27 614616.0 28 705272.0 29 826421.0 30 988175.0 31 1215197.0 32 1540576.0 33 2009128.0 34 2701359.0 35 4015657.0 36 6804661.0 37 7998887.0 38 2638451.0 39 3044.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 25.759099612654886 23.21702357948366 21.77332148537339 29.250555322488065 2 30.56580843286164 23.76361667023752 20.567035619045228 25.103539277855607 3 26.921189761365667 22.944486340179807 21.44073528514031 28.69358861331422 4 26.552470800220394 22.02612527377683 22.06029109964036 29.36111282636241 5 28.4867130768855 22.258119279297507 22.61701724285561 26.63815040096138 6 27.65310338681408 21.59538900696483 21.2510120473468 29.500495558874285 7 29.33193907500969 21.704406251019655 22.677994826471128 26.28565984749953 8 26.981866881240908 22.40771162008863 22.325352692269025 28.28506880640144 9 27.707707049042828 21.315922207998735 23.166929342423003 27.809441400535434 10-11 27.584518779658946 21.93318419833456 22.95245972473836 27.529837297268134 12-13 27.512588715045823 21.82728479079644 23.21644539671019 27.44368109744755 14-15 27.019564919795368 22.25181860845733 23.32637201584136 27.402244455905944 16-17 26.97192377538237 22.600279972800944 23.075679974705338 27.35211627711135 18-19 26.923328510776873 22.596572182783873 22.915990066924874 27.564109239514384 20-21 26.703483037576703 22.812895199051567 22.917287280300073 27.566334483071653 22-23 26.944111495539396 22.780669578426917 22.858861861663872 27.416357064369816 24-25 26.939666066675404 22.668772161111633 22.93211926939701 27.459442502815946 26-27 26.84955924118374 22.666074809582682 23.24074174137827 27.243624207855312 28-29 26.91750321240778 22.634314454478137 23.481971093726393 26.966211239387693 30-31 26.91235065788827 22.543966568419226 23.601291049864333 26.94239172382818 32-33 26.69495476205754 22.90185983836596 23.645684411737886 26.75750098783861 34-35 26.765921907416445 23.30221838508135 23.480513762282925 26.45134594521928 36-37 26.70811089404379 23.380974253859282 23.32849278743259 26.58242206466434 38-39 26.607884609278898 23.522951347911643 23.3328257878158 26.53633825499366 40-41 26.886043902388867 23.578632587035443 23.150491587136226 26.38483192343946 42-43 26.978600070553522 23.414235247497356 23.13693503982248 26.47022964212664 44-45 26.961873683441368 23.470395049739544 23.230110938980857 26.337620327838234 46-47 26.84271703490066 23.595865873726062 23.26631662183825 26.295100469535026 48-49 26.895797241563606 23.505053378088313 23.11039170441005 26.488757675938036 50-51 26.881126587090264 23.537773300545837 23.19652698067804 26.38457313168586 52-53 26.75496535017293 23.65718560839468 23.291168934549468 26.296680106882913 54-55 26.75571822551146 23.506365168096167 23.211487063864197 26.52642954252818 56-57 26.640202473022583 23.567349992942066 23.41152824503421 26.38091928900114 58-59 26.621238954194766 23.731864038428473 23.36661923119405 26.280277776182707 60-61 26.667211960921833 23.78357738561935 23.181318213412787 26.36789244004603 62-63 26.565725751946502 23.863577565800195 23.248414180436892 26.322282501816414 64-65 26.564955548221576 23.951485681378934 23.245105366502813 26.238453403896678 66-67 26.606591601573577 23.870346636019235 23.189640070141568 26.333421692265624 68-69 26.535787330791777 23.92159100357102 23.255129291988123 26.287492373649084 70-71 26.55702540279657 24.00387943418661 23.24058593353618 26.19850922948064 72-73 26.60518138322589 23.943762377800475 23.16075669376962 26.290299545204014 74-75 26.552111791719195 23.963394605629656 23.21977649737777 26.264717105273384 76-77 26.57946291121932 24.035562800100944 23.221043375359542 26.163930913320193 78-79 26.633813501936377 23.96480253972678 23.14774325450277 26.25364070383407 80-81 26.601789886858462 23.996397493065516 23.18282017346591 26.218992446610113 82-83 26.62805236869644 24.067031883880865 23.173378286165484 26.131537461257214 84-85 26.69348638606938 23.997635731929996 23.08867207799139 26.22020580400923 86-87 26.65856284627174 24.004286897025377 23.146987220266947 26.190163036435937 88-89 26.667980592849005 24.071583984951616 23.13572510602922 26.12471031617015 90-91 26.73962572685834 24.00456492948066 23.054750440545284 26.201058903115708 92-93 26.711482745184522 24.037382069675665 23.087743311417114 26.163391873722702 94-95 26.766256548944405 24.07902581561062 23.067196377750324 26.08752125769465 96-97 26.80793403984996 23.98857957200197 23.01803593037523 26.185450457772834 98-99 26.807184854826932 24.01673893971154 23.02565592287308 26.150420282588442 100-101 26.939997437559505 24.03100031267505 22.95188279296066 26.077119456804787 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3089.0 1 2600.5 2 2182.5 3 2308.5 4 2363.0 5 2432.5 6 2623.5 7 2809.5 8 2913.0 9 2962.0 10 2977.0 11 3102.5 12 3659.0 13 4568.0 14 5681.0 15 7629.5 16 10589.5 17 15179.5 18 22078.5 19 31798.0 20 44790.0 21 60606.5 22 79196.0 23 100008.0 24 123431.0 25 148862.0 26 175515.5 27 204212.5 28 235713.0 29 270582.5 30 308076.0 31 346842.0 32 386132.5 33 424726.0 34 460127.0 35 490136.0 36 514300.5 37 533513.0 38 546083.5 39 550530.5 40 549134.0 41 543836.5 42 535521.5 43 526620.0 44 521261.0 45 521316.5 46 529735.5 47 551268.0 48 586662.0 49 640631.0 50 716894.5 51 813681.5 52 921888.0 53 1030414.5 54 1125688.5 55 1195219.0 56 1234559.5 57 1238020.5 58 1213996.5 59 1185940.0 60 1171368.5 61 1179054.5 62 1210401.0 63 1257821.5 64 1298331.5 65 1314841.5 66 1299543.5 67 1239210.5 68 1132403.0 69 984674.5 70 804595.0 71 607427.5 72 421306.5 73 270888.5 74 164383.5 75 98848.0 76 61791.5 77 41832.5 78 31023.0 79 24280.0 80 19458.5 81 15965.0 82 13238.5 83 10876.0 84 9042.0 85 7527.0 86 6243.5 87 5257.0 88 4424.0 89 3648.5 90 2968.5 91 2436.5 92 1986.0 93 1574.5 94 1220.5 95 908.5 96 666.5 97 459.5 98 276.0 99 144.5 100 56.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026468618906396256 2 0.011187878085282139 3 0.0064146714118316515 4 0.008315314793115104 5 0.018147904558220688 6 0.03020781078576782 7 0.0479939451549374 8 0.05513755649993174 9 0.051733137034308964 10-11 0.04477986000519599 12-13 0.0605047142300333 14-15 0.06056005966940307 16-17 0.055133506833636395 18-19 0.057617302161449994 20-21 0.05927496556501255 22-23 0.06384568892369562 24-25 0.07227844403937582 26-27 0.07194637140315727 28-29 0.06649417068085338 30-31 0.0740305996564965 32-33 0.0741466900902965 34-35 0.07914262840999113 36-37 0.08324359047841381 38-39 0.08616070009982968 40-41 0.09257402162289621 42-43 0.08834751989931774 44-45 0.09319902012114496 46-47 0.09325841522681007 48-49 0.09340015354714726 50-51 0.08935318702932922 52-53 0.09176003869753122 54-55 0.09623626984265615 56-57 0.08665475938786216 58-59 0.09091905799686388 60-61 0.10437339931877593 62-63 0.10601621394592221 64-65 0.10888742734932413 66-67 0.10661691444639888 68-69 0.11707585259851831 70-71 0.10897247034152645 72-73 0.11447326705937451 74-75 0.11483908691472097 76-77 0.11624837078550215 78-79 0.10854050593668929 80-81 0.0998053757376231 82-83 0.09790878202263499 84-85 0.09443551822999129 86-87 0.10646167723841052 88-89 0.1122081537115097 90-91 0.09781698958660709 92-93 0.11173974231001443 94-95 0.11660744119702306 96-97 0.12663171516677513 98-99 0.12545191238606365 100-101 0.12961766911521191 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3.7040089E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.28051857358754 #Duplication Level Percentage of deduplicated Percentage of total 1 79.0254734890104 51.58823889885893 2 9.793096516879615 12.785968381261906 3 4.26360125595183 8.34990302938604 4 2.42247260547382 6.3256107166256275 5 1.5781291226325038 5.151054375076529 6 1.0087838638299012 3.9512360255689964 7 0.6294178581792826 2.8762106926994244 8 0.3783757835218063 1.976045389119281 9 0.26185797324566706 1.538480185749518 >10 0.6377694364608477 5.40673385289404 >50 9.275366722530339E-4 0.037740747338467655 >100 9.455814209539701E-5 0.012777705421278965 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.09605268497060036 0.0 0.03826394693598063 2 0.10052351656066486 8.099332590696528E-6 0.2877206909519035 3 0.1107475740676541 1.3498887651160881E-5 0.5115889435362858 4 0.11805587184199261 1.6198665181393057E-5 0.8066773273681929 5 0.1321352116621534 2.159822024185741E-5 1.0841361639276839 6 0.1473133609371187 2.159822024185741E-5 1.4243567287324823 7 0.1607312552623726 2.4297997772089585E-5 1.7390427976563447 8 0.17607409096668208 2.969755283255394E-5 2.054309318749207 9 0.19160861087563802 3.779688542325047E-5 2.3495974861183515 10-11 0.2132972736647582 3.779688542325047E-5 2.7895410834461005 12-13 0.246005078443521 3.779688542325047E-5 3.3881992022211396 14-15 0.279086802410221 3.914677418836655E-5 3.9663646596529505 16-17 0.3105689081902584 4.184655171859874E-5 4.5855437874352845 18-19 0.3423952356054004 4.859599554417917E-5 5.22573393384665 20-21 0.371158665412494 5.264566183952743E-5 5.878440248888171 22-23 0.3972992613489671 5.939510566510787E-5 6.516736501362079 24-25 0.4205888922135149 7.154410455115267E-5 7.144052758620532 26-27 0.44083587380149114 7.559377084650094E-5 7.773635479115614 28-29 0.4592470066689095 7.82935483767331E-5 8.415359099164151 30-31 0.47805905649956726 8.504299220231355E-5 9.059623209868636 32-33 0.49646883947822046 9.314232479301008E-5 9.69438545355547 34-35 0.5139417996538831 9.719199108835834E-5 10.320334273494861 36-37 0.5322638398628038 1.0124165738370661E-4 10.947514191988038 38-39 0.5503968956446081 1.0799110120928705E-4 11.582416824106442 40-41 0.5683247683341149 1.147405450348675E-4 12.225096165400682 42-43 0.5874229945829774 1.1879021133021576E-4 12.862197766317461 44-45 0.6076375788405908 1.2148998886044794E-4 13.494823136089117 46-47 0.6295260251669482 1.2688954392091228E-4 14.123586474103774 48-49 0.652314847299638 1.2823943268602838E-4 14.757843859392455 50-51 0.6765750481863043 1.4308820910230533E-4 15.395432770153441 52-53 0.7020811964031728 1.5253743045811794E-4 16.032693657944506 54-55 0.7300238938410758 1.579369855185823E-4 16.66169727615935 56-57 0.7598280878860739 1.592868742836984E-4 17.285081307445022 58-59 0.7918258511743856 1.6873609563951102E-4 17.909167280888553 60-61 0.8263681547849413 1.727857619348593E-4 18.53924406067167 62-63 0.8635629898189499 1.822349832906719E-4 19.16846771075523 64-65 0.9036047402585885 1.8763453835113624E-4 19.789871455222475 66-67 0.946856526181673 2.0113342600229712E-4 20.402830295575153 68-69 0.9924449695571735 2.0383320353252932E-4 21.01264659488264 70-71 1.041043664878883 2.0788286982787759E-4 21.62358735153147 72-73 1.0931183237707662 2.0788286982787759E-4 22.233237614520853 74-75 1.1485933524619771 2.1328242488834193E-4 22.833812575342357 76-77 1.2081463951126035 2.2003186871392237E-4 23.426060072371858 78-79 1.2703438698540923 2.2408153500927063E-4 24.014982793372877 80-81 1.336084802604011 2.267813125395028E-4 24.602527277944716 82-83 1.4055352296804686 2.3218086759996717E-4 25.18792813915755 84-85 1.478435972440563 2.4028020019066368E-4 25.76249749291909 86-87 1.555866941896387 2.45679755251128E-4 26.3292280426216 88-89 1.6371491439990868 2.6052853166740503E-4 26.89141351685197 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGGAG 11490 0.0 14.875307 3 CGGGAGA 14370 0.0 11.9270735 4 AGAGGGG 20060 0.0 11.906316 8 GGGAGAG 20965 0.0 10.326507 5 GAGGGGC 16215 0.0 10.015007 9 GAGAGGG 17010 0.0 9.546482 7 GGAGAGG 19010 0.0 8.716238 6 AGAGCGG 16505 0.0 7.3073273 8 ATCGGGA 19980 0.0 7.270764 2 ACGGGAG 7430 0.0 6.3899083 3 TAGGGAG 4745 0.0 5.3030176 3 GTCGGGA 14370 0.0 5.18677 2 GGGGAAG 30275 0.0 5.1372657 20-21 GTATCAT 13305 0.0 5.0874815 50-51 GGGAAGA 26560 0.0 5.060152 22-23 >>END_MODULE