FastQCFastQC Report
Thu 11 Sep 2014
QG2077_GCCAAT_L005_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameQG2077_GCCAAT_L005_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37040089
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGAG114900.014.8753073
CGGGAGA143700.011.92707354
AGAGGGG200600.011.9063168
GGGAGAG209650.010.3265075
GAGGGGC162150.010.0150079
GAGAGGG170100.09.5464827
GGAGAGG190100.08.7162386
AGAGCGG165050.07.30732738
ATCGGGA199800.07.2707642
ACGGGAG74300.06.38990833
TAGGGAG47450.05.30301763
GTCGGGA143700.05.186772
GGGGAAG302750.05.137265720-21
GTATCAT133050.05.087481550-51
GGGAAGA265600.05.06015222-23
Produced by FastQC (version 0.11.2)