FastQCFastQC Report
Thu 11 Sep 2014
QG2077_GCCAAT_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameQG2077_GCCAAT_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37040089
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC1238750.33443494155751086TruSeq Adapter, Index 6 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT482600.018.71550442-43
CGTATGC503750.018.05700344-45
CCGTCTT528850.017.27613650-51
TATGCCG534600.017.03271746-47
AGAGCAC888700.016.8359038
GAGCACA897500.016.7237749
TCTGCTT564300.016.32528756-57
GCCAATA562250.016.18215834-35
ATATCTC581300.015.578547538-39
CAATATC580000.015.57250536-37
AAGAGCA962350.015.522757
TCGTATG477650.015.41894142-43
ATCTCGT590000.015.3770840-41
GTCTTCT609250.015.17542452-53
AGTCACG604250.015.07695428-29
CTTGAAA593400.014.98840260-61
TGCTTGA607250.014.9672558-59
TCGGAAG1033950.014.6510343
GTATGCC505200.014.54517244-45
GAAGAGC1073450.014.0223726
Produced by FastQC (version 0.11.2)