FastQCFastQC Report
Thu 11 Sep 2014
CB4856_ACAGTG_L005_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCB4856_ACAGTG_L005_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42191975
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGT233250.013.3951929
AAGAGCG276750.011.39279658
GATCGGA290450.011.0985792
AGATCGG299050.010.7797691
GTAGGGA162950.09.55855718-19
TCGGAAG331700.09.5472374
CGGAAGA368150.08.7954265
GTGTAGG214000.08.02131316-17
TCGGGAG89150.07.82876633
AGAGGGG136800.07.8099158
ATCGGAA443350.07.5391663
CGTGTAG210950.07.49575916-17
GAAGAGC438950.07.4855637
AGCGTCG243350.07.31728210-11
GAGCGTC227000.07.18539710-11
GAGGGGC93600.07.15299659
TAGGGAA218350.07.0686220-21
GGAAGAG474400.07.0557446
GCCGTAT102350.06.93851948-49
CGTCGTG255050.06.879400312-13
Produced by FastQC (version 0.11.2)