FastQCFastQC Report
Thu 11 Sep 2014
CB4856_ACAGTG_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCB4856_ACAGTG_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42191975
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC443430.10509818514065766TruSeq Adapter, Index 5 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC194050.022.29954344-45
TATGCCG215450.020.19472146-47
CTCGTAT218950.019.3839242-43
CCAGTCA264550.016.50018926-27
GATCGGA347550.016.3826961
CACACGT272800.016.12349312-13
CCGTCTT281950.015.65898550-51
ACACAGT275650.015.61172532-33
AGTGATC275600.015.22703136-37
AGTCACA290200.014.9844828-29
TCGGAAG390150.014.9025953
CGTCTGA262950.014.68705116-17
CACGTCT273750.014.15116714-15
AGAGCAC404900.014.1005948
TGCCGTC312000.014.09752148-49
CTCCAGT318250.013.88767824-25
CGGAAGA419550.013.8346824
GTATGCC208200.013.483355544-45
TCGTATG213300.013.13872342-43
ATCTCGT333600.013.05675640-41
Produced by FastQC (version 0.11.2)