##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename lane7_Undetermined_L007_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12198817 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 8.900852353142112 2.0 2.0 15.0 2.0 33.0 2 10.029080360825152 2.0 2.0 15.0 2.0 33.0 3 9.187615815533588 2.0 2.0 15.0 2.0 33.0 4 10.287313105852805 2.0 2.0 15.0 2.0 37.0 5 9.87384883304668 2.0 2.0 15.0 2.0 37.0 6 10.203019850203507 2.0 2.0 15.0 2.0 37.0 7 11.050726722107562 2.0 2.0 15.0 2.0 37.0 8 9.964730186541859 2.0 2.0 15.0 2.0 37.0 9 11.746338108031296 2.0 2.0 15.0 2.0 37.0 10-11 11.667430456576241 2.0 2.0 15.0 2.0 37.0 12-13 12.228985031909241 2.0 2.0 21.0 2.0 37.0 14-15 12.738870785585192 2.0 2.0 27.0 2.0 37.0 16-17 11.342388323392342 2.0 2.0 15.0 2.0 37.0 18-19 12.855471313324891 8.5 2.0 18.5 2.0 37.0 20-21 12.737172752079157 4.0 2.0 21.0 2.0 37.0 22-23 14.325923448150752 8.5 2.0 27.0 2.0 37.0 24-25 15.070592951759174 15.0 2.0 27.0 2.0 37.0 26-27 14.725099286266857 10.5 2.0 27.0 2.0 37.0 28-29 13.707810191758758 10.5 2.0 22.0 2.0 37.0 30-31 13.471287584689565 8.5 2.0 24.5 2.0 37.0 32-33 15.385954597072814 15.0 2.0 27.0 2.0 37.0 34-35 14.984226749200353 6.0 2.0 27.0 2.0 37.0 36-37 18.319642224323882 15.0 6.0 27.0 2.0 37.0 38-39 17.6071702690515 15.0 4.0 33.0 2.0 37.0 40-41 19.798319009130147 18.5 10.5 33.0 2.0 37.0 42-43 17.437788270780683 18.5 2.0 30.0 2.0 37.0 44-45 16.42451255724223 15.0 4.0 27.0 2.0 37.0 46-47 17.857676568145912 15.0 4.0 27.0 2.0 37.0 48-49 17.98305741450175 18.5 2.0 27.0 2.0 37.0 50-51 18.39368981434839 18.5 4.0 27.0 2.0 35.0 52-53 16.560434261781285 15.0 2.0 27.0 2.0 35.0 54-55 15.867791278449378 15.0 2.0 27.0 2.0 37.0 56-57 17.320637689703844 15.0 8.5 27.0 4.0 37.0 58-59 18.428834328771387 18.5 8.5 27.0 4.0 37.0 60-61 20.068997428193242 18.5 15.0 27.0 6.0 35.0 62-63 18.428936182910196 22.0 6.0 27.0 2.0 33.0 64-65 18.17905383776148 18.5 8.5 27.0 2.0 33.0 66-67 19.000020780703572 18.5 15.0 27.0 2.0 33.0 68-69 17.216712981266955 15.0 6.0 27.0 2.0 33.0 70-71 19.588528092519134 18.5 15.0 27.0 4.0 33.0 72-73 18.37905433780997 18.5 8.5 27.0 4.0 33.0 74-75 18.495712453100985 22.0 6.0 27.0 2.0 33.0 76-77 19.736687172206942 22.0 10.5 27.0 4.0 33.0 78-79 20.715080240977464 22.0 15.0 27.0 6.0 33.0 80-81 20.64145765118044 22.0 15.0 27.0 6.0 33.0 82-83 20.70288639463974 22.0 15.0 27.0 6.0 33.0 84-85 21.918614526310215 24.5 15.0 27.0 6.0 33.0 86-87 21.255953302685008 24.5 15.0 27.0 6.0 33.0 88-89 20.11547754999522 22.0 15.0 27.0 4.0 33.0 90-91 20.638311813350427 22.0 15.0 27.0 6.0 33.0 92-93 20.558482105272994 22.0 15.0 27.0 6.0 33.0 94-95 21.061049280434325 22.0 15.0 27.0 6.0 33.0 96-97 21.745337560191288 27.0 15.0 27.0 6.0 33.0 98-99 21.427764061056084 27.0 15.0 27.0 6.0 33.0 100-101 20.343575815589332 24.5 15.0 27.0 4.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 11.561572363710429 2306 2 11.50380815888077 2306 3 10.963977427024979 2306 4 12.76921618549358 2306 5 12.737989867002877 2306 6 11.90938414028161 2306 7 11.888529564830243 2306 8 12.3403135975217 2306 9 11.334073849812668 2306 10-11 11.539510338593875 2306 12-13 11.257116545649318 2306 14-15 11.975827003466044 2306 16-17 11.140648366460915 2306 18-19 10.387417431948933 2306 20-21 10.417847499336684 2306 22-23 9.929429758460845 2306 24-25 9.545109622533197 2306 26-27 10.213430675405753 2306 28-29 9.506936393244196 2306 30-31 9.65819641495262 2306 32-33 9.264968050146003 2306 34-35 8.87219763525356 2306 36-37 8.346653079246444 2306 38-39 8.275456484375201 2306 40-41 6.9117889915018935 2306 42-43 7.285247834707583 2306 44-45 7.153078375285336 2306 46-47 7.00577580794242 2306 48-49 6.892574042544016 2306 50-51 6.387472380508392 2306 52-53 6.979150215837137 2306 54-55 6.9361255315051515 2306 56-57 6.936576758481525 2306 58-59 6.307106061443534 2306 60-61 5.849471959448689 2306 62-63 5.7352154068222525 2306 64-65 5.382618829481348 2306 66-67 5.156657860186254 2306 68-69 5.518542361951141 2306 70-71 5.044690354019366 2306 72-73 4.555248834573927 2306 74-75 4.030543720228199 2306 76-77 4.074206899036483 2306 78-79 4.000048073037554 2306 80-81 3.787643314121972 2306 82-83 3.6691047888971084 2306 84-85 3.1070495045311084 2306 86-87 3.189500685359569 2306 88-89 3.3685996108565064 2306 90-91 2.9635498414412638 2306 92-93 3.1366356684569077 2306 94-95 3.058800168582291 2306 96-97 2.4539981217153226 2306 98-99 2.4244799163593846 2306 100-101 2.3557181371434943 2307 1 11.401419097480343 2307 2 11.273080711837743 2307 3 10.678314629200877 2307 4 12.404134006641033 2307 5 12.567631963402075 2307 6 11.427987377402737 2307 7 11.60098274781344 2307 8 12.053146082873013 2307 9 11.145276033695964 2307 10-11 11.244157729941836 2307 12-13 10.94110759324004 2307 14-15 11.793529466086667 2307 16-17 10.930131715461272 2307 18-19 10.22028386361449 2307 20-21 10.322019633551324 2307 22-23 9.885906979606848 2307 24-25 9.617566255109232 2307 26-27 10.316326845499908 2307 28-29 9.627692121396187 2307 30-31 9.686897431295812 2307 32-33 9.435711073540226 2307 34-35 9.441541945293817 2307 36-37 8.666847045306874 2307 38-39 8.428360248314878 2307 40-41 7.3024689880058276 2307 42-43 7.616880948814959 2307 44-45 7.841929226669425 2307 46-47 7.783776838297335 2307 48-49 7.5855138342820645 2307 50-51 6.878181219276687 2307 52-53 7.6112460398428325 2307 54-55 7.550009890011271 2307 56-57 7.665792579532656 2307 58-59 7.11038754004651 2307 60-61 6.643244489884584 2307 62-63 6.617855300460381 2307 64-65 6.507954563544974 2307 66-67 6.167908058883295 2307 68-69 6.433680709370648 2307 70-71 5.6799539922477535 2307 72-73 5.306679266152646 2307 74-75 4.832896984174987 2307 76-77 4.782337961167958 2307 78-79 4.763746113292019 2307 80-81 4.476984802165088 2307 82-83 4.508634605556818 2307 84-85 3.6930689651132305 2307 86-87 3.8610012373443325 2307 88-89 4.208323718603829 2307 90-91 3.7822538837813866 2307 92-93 3.9126818021679775 2307 94-95 3.5697013373576176 2307 96-97 3.161345547025821 2307 98-99 3.0890045176115315 2307 100-101 3.026150996631678 2201 1 11.153107632561248 2201 2 10.728551961740468 2201 3 10.505977478184711 2201 4 12.166919023547582 2201 5 12.11511900522105 2201 6 11.23320470921896 2201 7 10.563602666688496 2201 8 11.619782631487134 2201 9 10.939696972777526 2201 10-11 11.137642690234504 2201 12-13 10.866068425422405 2201 14-15 11.331935983207302 2201 16-17 10.864203862430095 2201 18-19 9.821889592056785 2201 20-21 9.695775651663109 2201 22-23 9.274822316853633 2201 24-25 9.277564774858085 2201 26-27 9.846489522208834 2201 28-29 9.371282796817873 2201 30-31 9.25541936379443 2201 32-33 9.020025279633138 2201 34-35 9.070648308402514 2201 36-37 8.351367181825047 2201 38-39 8.167559500922675 2201 40-41 7.342551283720113 2201 42-43 7.572892608393893 2201 44-45 7.669083353590008 2201 46-47 7.590721722593592 2201 48-49 7.313811899820191 2201 50-51 6.479563497276498 2201 52-53 7.509931599761877 2201 54-55 7.607434360055262 2201 56-57 7.394718264243551 2201 58-59 6.8781110294648435 2201 60-61 6.526835310904733 2201 62-63 6.417752961022007 2201 64-65 6.360405045687006 2201 66-67 5.918635054000394 2201 68-69 6.2779248061420425 2201 70-71 5.682909649567854 2201 72-73 5.353099765912422 2201 74-75 4.796532984268463 2201 76-77 4.8217231293971885 2201 78-79 4.993404859723071 2201 80-81 4.543773323341007 2201 82-83 4.591649436689348 2201 84-85 3.7278007080702302 2201 86-87 4.044305734645157 2201 88-89 4.316870646504299 2201 90-91 3.9393387553587154 2201 92-93 3.879571013995907 2201 94-95 3.7464471885407526 2201 96-97 3.5833867558481742 2201 98-99 3.456764695850655 2201 100-101 3.1555030156121333 1101 1 10.215676187398845 1101 2 10.716971223713774 1101 3 9.548469427769785 1101 4 11.795718545279154 1101 5 7.93689562951355 1101 6 9.543227319014868 1101 7 10.150980726631872 1101 8 11.250866562832165 1101 9 10.049261855055931 1101 10-11 7.767904527258224 1101 12-13 9.858870528613998 1101 14-15 10.52059422789059 1101 16-17 9.91573014696965 1101 18-19 5.89743448630599 1101 20-21 8.874342920210065 1101 22-23 8.742116488859171 1101 24-25 8.724470088110799 1101 26-27 9.406702919333846 1101 28-29 8.442984075089456 1101 30-31 8.784586676988802 1101 32-33 8.604522997020418 1101 34-35 8.34167862128528 1101 36-37 7.854482564505325 1101 38-39 7.574725240465099 1101 40-41 6.856455927684536 1101 42-43 7.077631304094908 1101 44-45 7.053765607951409 1101 46-47 7.201633514089075 1101 48-49 6.997690032273315 1101 50-51 6.359799385109472 1101 52-53 7.172979807685749 1101 54-55 7.150880598617011 1101 56-57 6.964135626165316 1101 58-59 6.488624440105273 1101 60-61 6.152101796585928 1101 62-63 6.171095104043939 1101 64-65 6.103090861975613 1101 66-67 5.6177409044313045 1101 68-69 6.0765678413933415 1101 70-71 5.369919231002651 1101 72-73 4.975356127850677 1101 74-75 4.714468765429654 1101 76-77 4.7641782004785895 1101 78-79 4.732166587594591 1101 80-81 4.2543782590497266 1101 82-83 4.416922542673241 1101 84-85 3.875190719791579 1101 86-87 3.9075731595003163 1101 88-89 4.144016005097804 1101 90-91 3.7774897946581554 1101 92-93 3.9770002542698393 1101 94-95 3.7173903487850595 1101 96-97 3.278704993341087 1101 98-99 3.2825945027013006 1101 100-101 3.1503893514623798 2304 1 4.603666826018639 2304 2 7.464030047243185 2304 3 8.533105696037428 2304 4 7.3898565958239 2304 5 9.642288019292803 2304 6 9.603177605315835 2304 7 9.932769916697328 2304 8 9.579305854542383 2304 9 8.976105855033573 2304 10-11 9.627688231674135 2304 12-13 9.407316323353072 2304 14-15 9.776457947511282 2304 16-17 8.98871211177876 2304 18-19 8.36313445630634 2304 20-21 8.19017420853648 2304 22-23 7.707594675762561 2304 24-25 7.5573345139343395 2304 26-27 8.558441501300887 2304 28-29 8.018016450483778 2304 30-31 7.980376711143673 2304 32-33 7.8076934564994644 2304 34-35 7.66744431100695 2304 36-37 7.133680232358934 2304 38-39 7.088558664170037 2304 40-41 6.081887894636473 2304 42-43 6.054906479108368 2304 44-45 5.9765257430802485 2304 46-47 6.588514538474502 2304 48-49 6.382596204188058 2304 50-51 5.676835131271474 2304 52-53 6.292895193777465 2304 54-55 6.377395347821832 2304 56-57 6.661341885842997 2304 58-59 5.894588174073633 2304 60-61 5.473450085022275 2304 62-63 5.52918778804332 2304 64-65 5.381838115890009 2304 66-67 5.116000505715512 2304 68-69 5.510705994421347 2304 70-71 4.882593988257071 2304 72-73 4.769460498668664 2304 74-75 4.093342027173769 2304 76-77 4.0412949456847045 2304 78-79 4.132140733862432 2304 80-81 3.7718231463460903 2304 82-83 3.9347245841398326 2304 84-85 3.3664336181673136 2304 86-87 3.4143753463068087 2304 88-89 3.8693029116498394 2304 90-91 3.6513260972111077 2304 92-93 3.5690372581809733 2304 94-95 3.494291599639862 2304 96-97 3.3068757789032404 2304 98-99 3.209341423079259 2304 100-101 3.039545830361078 2202 1 9.247779071372392 2202 2 9.386364799216626 2202 3 8.323283889977336 2202 4 8.346435145445312 2202 5 9.056865351452995 2202 6 8.441617177376141 2202 7 9.205356107653834 2202 8 8.880747746219498 2202 9 7.448319588037677 2202 10-11 8.370523856750097 2202 12-13 8.234589246579262 2202 14-15 8.602854859605813 2202 16-17 8.169447086468658 2202 18-19 7.957690862053347 2202 20-21 8.274764390522005 2202 22-23 7.88922404815715 2202 24-25 4.380171001205532 2202 26-27 7.698808443281379 2202 28-29 7.296137077829496 2202 30-31 7.3762937232943955 2202 32-33 7.237201413107151 2202 34-35 7.484142825226421 2202 36-37 6.725462161370139 2202 38-39 6.1448126700018015 2202 40-41 5.71729773342674 2202 42-43 6.22451770696787 2202 44-45 -1.1100514872785787 2202 46-47 3.400285191426377 2202 48-49 1.535495231777265 2202 50-51 4.403355944976017 2202 52-53 1.6953532033114769 2202 54-55 4.6515079359761025 2202 56-57 1.25752890533796 2202 58-59 4.4260567938029425 2202 60-61 5.095349064827168 2202 62-63 5.021810798891398 2202 64-65 4.854016348265127 2202 66-67 4.716792894297555 2202 68-69 5.266420063473838 2202 70-71 4.5350779155875145 2202 72-73 4.3303047332314755 2202 74-75 3.9637426311259496 2202 76-77 3.9741963157440914 2202 78-79 -0.4607109145405843 2202 80-81 2.4080607964381784 2202 82-83 3.3487458003752835 2202 84-85 2.891308444040238 2202 86-87 3.0630888055898993 2202 88-89 3.300616518025514 2202 90-91 2.932843695862342 2202 92-93 3.058126125578461 2202 94-95 3.0734661567779575 2202 96-97 2.838507436634586 2202 98-99 2.833610868438413 2202 100-101 2.6505035267000707 1102 1 -16.981281270867175 1102 2 -4.484343104312883 1102 3 2.469597434967902 1102 4 4.743821294275964 1102 5 4.590885296477083 1102 6 5.199992190199815 1102 7 7.0927395678197485 1102 8 5.994320990548658 1102 9 6.56901684916749 1102 10-11 5.393573543919121 1102 12-13 4.784481483251557 1102 14-15 5.806860214261139 1102 16-17 5.21457636300444 1102 18-19 5.040349130097546 1102 20-21 4.621995243677894 1102 22-23 4.747684466933073 1102 24-25 5.317193031028481 1102 26-27 6.486368361658897 1102 28-29 2.0827538795277434 1102 30-31 4.096569195280651 1102 32-33 4.283941776678198 1102 34-35 4.608748153361891 1102 36-37 5.419953738647134 1102 38-39 5.188831246796745 1102 40-41 4.008798535191232 1102 42-43 3.408007182551536 1102 44-45 3.5669243395247285 1102 46-47 1.3768456256407333 1102 48-49 3.352717324265331 1102 50-51 3.9231924332575367 1102 52-53 3.8344267487955364 1102 54-55 1.8434154941472336 1102 56-57 3.4101149917924793 1102 58-59 4.012072919567096 1102 60-61 3.8252173402615846 1102 62-63 0.09773215005713354 1102 64-65 2.0136291416410756 1102 66-67 2.449423664381598 1102 68-69 3.617023723725758 1102 70-71 -0.723862450406557 1102 72-73 -2.402064144901768 1102 74-75 0.5036840573586687 1102 76-77 1.1960929892133727 1102 78-79 2.6124716442390827 1102 80-81 2.590267487734451 1102 82-83 0.2988720420209994 1102 84-85 0.6215732947448736 1102 86-87 1.6100783346912237 1102 88-89 2.4048391221267984 1102 90-91 2.3084660261621934 1102 92-93 2.5498530908406707 1102 94-95 2.5854514161374063 1102 96-97 2.0440844764380515 1102 98-99 2.002086196577217 1102 100-101 1.8213832313493086 2305 1 8.425173974019295 2305 2 9.482640204760873 2305 3 9.51088858537896 2305 4 10.63744263188304 2305 5 10.835282348811834 2305 6 9.220022823197233 2305 7 5.9868706101024145 2305 8 8.90721474375016 2305 9 9.272733889533058 2305 10-11 7.5922658090872694 2305 12-13 8.061258729629103 2305 14-15 8.479979759026396 2305 16-17 8.267122857707061 2305 18-19 7.453875576416262 2305 20-21 8.313437530400723 2305 22-23 8.040574467085225 2305 24-25 7.885092794180636 2305 26-27 8.748740745301689 2305 28-29 7.840278997064122 2305 30-31 8.225316538983897 2305 32-33 7.982576985352814 2305 34-35 8.103007002190079 2305 36-37 6.837838033149929 2305 38-39 7.086173935704455 2305 40-41 6.1530487621492895 2305 42-43 6.386023949553106 2305 44-45 5.728018911296726 2305 46-47 6.0430334404023895 2305 48-49 5.832671476202972 2305 50-51 4.385145895445564 2305 52-53 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-2.1030147944979056 1209 78-79 -1.48534436504654 1209 80-81 -1.543376453305747 1209 82-83 -1.995429168247945 1209 84-85 -2.1131661991835706 1209 86-87 -1.746056098938375 1209 88-89 -1.8709668852357808 1209 90-91 -1.545366065038941 1209 92-93 -1.875939988568632 1209 94-95 -1.3272833887255082 1209 96-97 -1.3299411611204732 1209 98-99 -1.2921565722591026 1209 100-101 -1.167881202486555 1315 1 -16.981281270867175 1315 2 -17.033028776286226 1315 3 -17.585752435450463 1315 4 -19.28505689898579 1315 5 -19.250099523959793 1315 6 -20.0978604569679 1315 7 -19.945949806486063 1315 8 -19.523635321928204 1315 9 -20.391368193409335 1315 10-11 -20.23424948458884 1315 12-13 -20.468202707788798 1315 14-15 -20.728126827575217 1315 16-17 -21.354194462615702 1315 18-19 -21.989547204593308 1315 20-21 -21.87383963229397 1315 22-23 -22.18808006472187 1315 24-25 -17.553283120737106 1315 26-27 -18.72597476415237 1315 28-29 -15.651470340442053 1315 30-31 -21.528014292316534 1315 32-33 -21.606839415472855 1315 34-35 -18.92133210507682 1315 36-37 -22.359572362796776 1315 38-39 -22.37882887908256 1315 40-41 -19.7273332696119 1315 42-43 -20.18644196957898 1315 44-45 -22.405308166954352 1315 46-47 -17.0797087398864 1315 48-49 -16.30553317784694 1315 50-51 -19.08134053826631 1315 52-53 -21.327087505184473 1315 54-55 -18.3294148197381 1315 56-57 -22.0287965224615 1315 58-59 -22.439148850147824 1315 60-61 -22.533816350963885 1315 62-63 -22.3921789939636 1315 64-65 -22.14973627857366 1315 66-67 -10.652534728116894 1315 68-69 -21.65815213706654 1315 70-71 -22.03254442004743 1315 72-73 -12.682805565696153 1315 74-75 -14.042931312711112 1315 76-77 -11.727708943952017 1315 78-79 -12.68487973008617 1315 80-81 -21.600495192754256 1315 82-83 -14.044437781353887 1315 84-85 -11.883651235372586 1315 86-87 -12.136932760819239 1315 88-89 -9.528773947902067 1315 90-91 -12.263052345447718 1315 92-93 -15.390023283064867 1315 94-95 -20.744981756761522 1315 96-97 -17.035938303068168 1315 98-99 -9.095862101542721 1315 100-101 -5.499611291142585 1212 1 -16.981281270867175 1212 2 -17.033028776286226 1212 3 -17.585752435450463 1212 4 -19.28505689898579 1212 5 -19.250099523959793 1212 6 -20.0978604569679 1212 7 -19.945949806486063 1212 8 -19.523635321928204 1212 9 -20.391368193409335 1212 10-11 -20.23424948458884 1212 12-13 -20.468202707788798 1212 14-15 -20.728126827575217 1212 16-17 -21.354194462615702 1212 18-19 -17.007175409721512 1212 20-21 -21.87383963229397 1212 22-23 -22.18808006472187 1212 24-25 -18.70766329262921 1212 26-27 -21.283254000429217 1212 28-29 -16.628045961409082 1212 30-31 -11.793416713968956 1212 32-33 -21.606839415472855 1212 34-35 -17.582111271658494 1212 36-37 -22.359572362796776 1212 38-39 -22.37882887908256 1212 40-41 -17.656151715375454 1212 42-43 -14.078355629153739 1212 44-45 -10.9776016142478 1212 46-47 -15.2398002615531 1212 48-49 -17.017238610331013 1212 50-51 -10.824729355760141 1212 52-53 -5.450663003759971 1212 54-55 -4.714938961467492 1212 56-57 -5.129490966905944 1212 58-59 -3.5325427247916963 1212 60-61 -13.368218060365594 1212 62-63 -10.567125575160182 1212 64-65 -14.793343828431208 1212 66-67 -10.955499237972813 1212 68-69 -7.563244729659132 1212 70-71 -14.174727468480478 1212 72-73 -10.565172260920843 1212 74-75 -7.890859623571998 1212 76-77 -16.67964978464584 1212 78-79 -9.559359268602103 1212 80-81 -7.682671118680183 1212 82-83 -5.552352008689354 1212 84-85 -4.998455255934363 1212 86-87 -3.8681848006780797 1212 88-89 -3.7357166074700245 1212 90-91 -4.498118581364196 1212 92-93 -4.509734651538167 1212 94-95 -4.756288736818501 1212 96-97 -13.004244081308313 1212 98-99 -9.768295321663889 1212 100-101 -7.011596481767393 1213 1 -16.981281270867175 1213 2 -17.033028776286226 1213 3 -17.585752435450463 1213 4 -19.28505689898579 1213 5 -19.250099523959793 1213 6 -20.0978604569679 1213 7 -19.945949806486063 1213 8 -19.523635321928204 1213 9 -20.391368193409335 1213 10-11 -20.23424948458884 1213 12-13 -20.468202707788798 1213 14-15 -20.728126827575217 1213 16-17 -21.354194462615702 1213 18-19 -21.989547204593308 1213 20-21 -21.87383963229397 1213 22-23 -22.18808006472187 1213 24-25 -22.166886939352857 1213 26-27 -21.283254000429217 1213 28-29 -21.757675591038712 1213 30-31 -21.528014292316534 1213 32-33 -21.606839415472855 1213 34-35 -21.56118890698613 1213 36-37 -22.359572362796776 1213 38-39 -22.37882887908256 1213 40-41 -16.96905147862733 1213 42-43 -22.72009352089163 1213 44-45 -22.405308166954352 1213 46-47 -22.275412797165636 1213 48-49 -22.218182342524745 1213 50-51 -21.861054142084928 1213 52-53 -21.327087505184473 1213 54-55 -22.003400499929032 1213 56-57 -17.673994985969824 1213 58-59 -22.439148850147824 1213 60-61 -16.498236965560558 1213 62-63 -22.3921789939636 1213 64-65 -22.14973627857366 1213 66-67 -16.164485817381927 1213 68-69 -21.65815213706654 1213 70-71 -22.03254442004743 1213 72-73 -21.990920124168706 1213 74-75 -16.19135142767296 1213 76-77 -15.892281473509923 1213 78-79 -15.692629379425032 1213 80-81 -15.828136037824677 1213 82-83 -8.231307508067388 1213 84-85 -16.14635735383646 1213 86-87 -21.473686937430216 1213 88-89 -8.945749766798961 1213 90-91 -3.7729934359045387 1213 92-93 -15.04095104534259 1213 94-95 -5.139267928336427 1213 96-97 -13.821552639990111 1213 98-99 -8.55600892847961 1213 100-101 -14.74133358738478 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 19166.0 3 26317.0 4 193866.0 5 211245.0 6 254286.0 7 526006.0 8 775069.0 9 705781.0 10 909108.0 11 1072691.0 12 739360.0 13 636155.0 14 570004.0 15 673440.0 16 640621.0 17 350512.0 18 237786.0 19 245964.0 20 251975.0 21 237544.0 22 197348.0 23 147441.0 24 127605.0 25 131678.0 26 138578.0 27 144387.0 28 147558.0 29 149132.0 30 154019.0 31 165806.0 32 185419.0 33 213347.0 34 240618.0 35 262703.0 36 269302.0 37 202131.0 38 44732.0 39 117.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 28.60525312276479 27.864231477251693 21.611002735768704 21.91951266421481 2 23.863112450453432 30.20230575358313 25.768989930682785 20.16559186528065 3 20.526268759254876 28.00061041559322 28.531126553310404 22.941994271841505 4 22.896938267591754 27.281188732337135 27.566511920845986 22.255361079225118 5 22.695230531900442 28.450341360697053 27.16290455032992 21.69152355707259 6 22.467068650286436 28.118321913774864 26.655786341311007 22.758823094627694 7 22.927365964696538 28.20058543114318 27.58618915728916 21.28585944687113 8 21.993149993905792 27.794864886458203 27.93272811144294 22.279257008193063 9 22.17218636226377 27.28698617642971 28.192071836356853 22.34875562494966 10-11 22.75382984168769 27.856972418982085 27.168149341215276 22.221048398114952 12-13 22.50956955604565 27.907931592630636 27.2902966582972 22.292202193026505 14-15 22.336255295735043 28.105783498952047 27.24558034529747 22.312380860015445 16-17 22.59501706658062 28.038223755368662 27.177446620656347 22.189312557394373 18-19 22.745851758223317 27.92466201230846 27.07684329213569 22.252642937332535 20-21 22.663533579434088 27.984787147308953 26.996391734099735 22.35528753915722 22-23 22.057899823691933 27.853309757319845 27.707855419338966 22.380934999649256 24-25 22.41284322135078 27.978445471521713 27.430171422375803 22.178539884751707 26-27 22.534244015370763 27.997185101027377 27.242511531607107 22.226059351994753 28-29 22.793270648158924 28.048128040906565 27.030517477511673 22.128083833422835 30-31 22.806583201234158 27.793263119130046 27.00566973986594 22.394483939769856 32-33 22.5001256988042 28.115341355224555 27.089745104023294 22.294787841947947 34-35 22.737998388287505 27.846020767891122 27.17622286188822 22.23975798193315 36-37 22.543622836251906 28.014553687326494 27.14523693276762 22.296586543653984 38-39 22.51620366872822 28.07329163839509 27.24483928802127 22.165665404855414 40-41 22.578287523649333 27.997841906440645 27.271962684299 22.151907885611017 42-43 22.466022836303708 28.085281861120258 27.17066423163066 22.278031070945374 44-45 22.501036072336017 27.95922842623717 27.164603491322293 22.375132010104522 46-47 22.389790972542663 28.16137972954517 26.656894341120346 22.791934956791813 48-49 22.52835168635606 27.961124932180816 26.98104905512252 22.52947432634061 50-51 22.48124613122715 27.999844638815897 27.23693117613461 22.281978053822343 52-53 22.5245355007747 28.077014935000655 27.27138111295247 22.127068451272176 54-55 22.363889609432725 27.940513206930795 27.166782793776434 22.528814389860045 56-57 22.332523852269013 28.03324738671604 27.20011538229236 22.43411337872259 58-59 22.451502381749172 28.089399103899986 27.254110575431355 22.204987938919487 60-61 21.989719836834233 27.878365466820863 27.056970005760743 23.07494469058416 62-63 22.3676062258344 28.008192571044766 27.316798791028408 22.307402412092422 64-65 22.484083041503858 27.888707585569428 27.29971249091655 22.327496882010163 66-67 22.477996170673105 27.98264533703021 27.15453371669024 22.38482477560645 68-69 22.408151409038034 27.946701657897616 27.253892643352255 22.391254289712094 70-71 22.65341929374072 27.9773677649736 27.13101250824027 22.238200433045417 72-73 22.341618911292844 27.946666341702215 27.495972946309998 22.21574180069494 74-75 22.531227534967908 27.948092515140033 27.293222656585996 22.227457293306067 76-77 22.741352470361452 28.07638352119367 27.079679407442374 22.1025846010025 78-79 22.685424398187568 28.03722687454756 27.13934876353366 22.13799996373121 80-81 22.723161441427404 27.969180027879187 27.083821080782755 22.22383744991065 82-83 22.749569181444958 28.022131461763184 26.98163683307565 22.24666252371621 84-85 22.815600311454496 27.95515788688501 27.071313036562568 22.157928765097928 86-87 22.7858581642452 28.074694677796437 27.05618709288643 22.08326006507193 88-89 22.875071485045943 28.03775305527243 26.71485565100421 22.372319808677418 90-91 22.881898203222605 28.116233720910806 26.660683147380897 22.341184928485696 92-93 23.05262224733474 27.951237193691732 26.910915471724266 22.085225087249263 94-95 22.963621767766142 27.914991242400443 27.149325120624585 21.97206186920883 96-97 23.222364164646763 27.978825403277717 26.942694949357943 21.856115482717577 98-99 23.292236640099865 28.011497802231005 26.91035678113236 21.785908776536765 100-101 23.63102256949424 27.93373046027627 26.516248899996615 21.918998070232877 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9896.0 1 8856.5 2 7864.5 3 10231.0 4 16476.0 5 25747.0 6 37624.5 7 51293.0 8 65430.5 9 80248.0 10 96969.0 11 115529.0 12 136875.0 13 161156.0 14 186030.5 15 211260.5 16 236937.5 17 260975.0 18 282747.5 19 302576.0 20 318382.0 21 329766.0 22 336608.5 23 338872.0 24 338484.0 25 335533.5 26 330036.5 27 323537.5 28 316544.5 29 307881.0 30 299025.0 31 291007.5 32 281406.0 33 272414.0 34 266735.0 35 261426.0 36 257652.0 37 258978.5 38 263475.0 39 264533.0 40 265544.0 41 275075.0 42 285392.0 43 288680.0 44 287624.5 45 284189.0 46 275795.5 47 262053.0 48 233442.0 49 192402.5 50 156971.0 51 130455.5 52 109637.0 53 95804.0 54 86872.0 55 75875.0 56 64777.5 57 56367.0 58 50340.5 59 45945.0 60 42383.5 61 38866.5 62 35465.0 63 32363.0 64 29370.0 65 26820.5 66 24428.0 67 21856.0 68 19589.0 69 17533.0 70 15424.5 71 13486.5 72 11700.5 73 10039.0 74 8509.0 75 7131.0 76 5875.5 77 4706.0 78 3733.0 79 2976.5 80 2391.5 81 1901.0 82 1482.0 83 1192.5 84 975.5 85 770.0 86 602.5 87 477.0 88 368.5 89 281.0 90 220.5 91 163.5 92 117.5 93 92.0 94 73.0 95 55.5 96 36.0 97 23.0 98 13.5 99 7.5 100 2.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 72.84942466142414 2 63.265798642606086 3 71.74453883520016 4 67.72113230323892 5 70.9115564238729 6 69.72233455096506 7 63.34978219609327 8 71.88673295123618 9 57.35249573790638 10-11 57.400049529392895 12-13 55.22744951416191 14-15 58.07579538245389 16-17 62.24880658509756 18-19 51.21966744808124 20-21 51.74772275049294 22-23 43.96605424935877 24-25 38.15389229955659 26-27 43.7423440322123 28-29 42.901541190428546 30-31 44.439399328639816 32-33 38.12675032341251 34-35 46.64560506153998 36-37 18.9378117566646 38-39 30.011844591159946 40-41 14.959204650746052 42-43 31.00048963764273 44-45 28.711907064430918 46-47 23.289955083349476 48-49 26.614908642370814 50-51 24.441595443230273 52-53 30.357615824550855 54-55 33.05042202043034 56-57 21.280399566613713 58-59 19.617504713776754 60-61 7.519450451629858 62-63 23.639935741309998 64-65 20.91001119206887 66-67 13.041817907424957 68-69 24.439005028110515 70-71 9.17991064215489 72-73 17.93178387707595 74-75 21.375203841487252 76-77 9.487194536978464 78-79 5.523125726043763 80-81 8.139858971570767 82-83 3.5268296917643736 84-85 1.6417534585525793 86-87 7.18323752213022 88-89 8.418029387603733 90-91 3.3408157528717743 92-93 5.726592176929944 94-95 3.7463878669546395 96-97 1.9717116831902632 98-99 3.2764857444783377 100-101 6.9724506892758535 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.2198817E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.91056848670108 #Duplication Level Percentage of deduplicated Percentage of total 1 96.91391969750683 53.215984248645974 2 1.6599315601659652 1.8229557123545965 3 0.2393559529566581 0.3942951434257853 4 0.14029834728319954 0.30815448028260406 5 0.02694702299603714 0.07398381758683031 6 0.029951961845034736 0.09868075513217024 7 0.012841195558014855 0.04935821436976475 8 0.0112401075124739 0.04937605546892651 9 0.00999481767961798 0.04939390086378786 >10 0.13268417185023754 2.622255021477943 >50 0.5700657533457807 23.5815513412488 >100 0.2527694113001187 17.734011309142765 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 4.0987581008879794E-5 0.0 0.0 2 4.0987581008879794E-5 8.19751620177596E-6 0.0 3 4.0987581008879794E-5 8.19751620177596E-6 0.0 4 6.558012961420768E-5 8.19751620177596E-6 0.0 5 9.017267821953555E-5 8.19751620177596E-6 0.0 6 1.0656771062308747E-4 1.639503240355192E-5 0.0 7 1.1476522682486343E-4 1.639503240355192E-5 0.0 8 1.4755529163196725E-4 1.639503240355192E-5 0.0 9 1.4755529163196725E-4 1.639503240355192E-5 0.0 10-11 1.5165404973285524E-4 1.639503240355192E-5 8.19751620177596E-6 12-13 1.6395032403551917E-4 1.639503240355192E-5 1.639503240355192E-5 14-15 1.7214784023729515E-4 1.639503240355192E-5 1.639503240355192E-5 16-17 1.9264163074173503E-4 2.0493790504439897E-5 1.639503240355192E-5 18-19 2.213329374479509E-4 2.4592548605327878E-5 1.639503240355192E-5 20-21 2.869130670621586E-4 2.4592548605327878E-5 1.639503240355192E-5 22-23 2.951105832639345E-4 2.4592548605327878E-5 2.4592548605327878E-5 24-25 3.033080994657105E-4 2.4592548605327878E-5 2.4592548605327878E-5 26-27 3.401969223737023E-4 2.4592548605327878E-5 2.4592548605327878E-5 28-29 3.8528326148347007E-4 2.869130670621586E-5 2.4592548605327878E-5 30-31 5.369373112163253E-4 4.0987581008879794E-5 2.4592548605327878E-5 32-33 7.99257829673156E-4 4.0987581008879794E-5 3.279006480710384E-5 34-35 0.001233726188367282 4.0987581008879794E-5 4.0987581008879794E-5 36-37 0.0018567374197022547 4.0987581008879794E-5 4.0987581008879794E-5 38-39 0.0027379704113931706 4.0987581008879794E-5 4.5086339109767774E-5 40-41 0.003754462420413389 4.0987581008879794E-5 4.9185097210655755E-5 42-43 0.005037373705991327 4.0987581008879794E-5 4.9185097210655755E-5 44-45 0.0069555924972069015 4.0987581008879794E-5 4.9185097210655755E-5 46-47 0.009382057292932584 4.0987581008879794E-5 4.9185097210655755E-5 48-49 0.012509409723910113 4.0987581008879794E-5 4.9185097210655755E-5 50-51 0.016542587695183887 4.0987581008879794E-5 4.9185097210655755E-5 52-53 0.021288949576012163 4.0987581008879794E-5 4.9185097210655755E-5 54-55 0.027375605355830814 4.0987581008879794E-5 4.9185097210655755E-5 56-57 0.034757468695530064 4.0987581008879794E-5 4.9185097210655755E-5 58-59 0.04410673592365555 4.5086339109767774E-5 4.9185097210655755E-5 60-61 0.06019026271153998 4.9185097210655755E-5 5.738261341243172E-5 62-63 0.08104474392885802 6.14813715133197E-5 6.14813715133197E-5 64-65 0.10457571418605591 6.558012961420768E-5 8.197516201775959E-5 66-67 0.13419743898117334 7.377764581598363E-5 8.197516201775959E-5 68-69 0.17358650433070683 7.377764581598363E-5 9.017267821953555E-5 70-71 0.2225543673620155 7.78764039168716E-5 9.017267821953555E-5 72-73 0.2753627667338563 8.197516201775959E-5 9.017267821953555E-5 74-75 0.3343848833866432 8.197516201775959E-5 1.024689525221995E-4 76-77 0.4162452801775779 8.197516201775959E-5 1.1476522682486343E-4 78-79 0.5131563167149733 8.197516201775959E-5 1.1476522682486343E-4 80-81 0.6170598345724836 9.427143632042352E-5 1.1476522682486343E-4 82-83 0.7352967095087991 1.1066646872397545E-4 1.1886398492575141E-4 84-85 0.8643625033476607 1.1476522682486343E-4 1.2296274302663938E-4 86-87 1.0077534567491258 1.1476522682486343E-4 1.3525901732930334E-4 88-89 1.1587230138791327 1.1476522682486343E-4 1.4755529163196728E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTTAGC 2010 0.0028984377 5.5956154 6 TGAACCG 1335 7.429331E-4 5.0610223 12-13 >>END_MODULE