##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename lane7_Undetermined_L007_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12198817 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.073757725851614 33.0 33.0 33.0 27.0 33.0 2 31.03514340775831 33.0 33.0 33.0 27.0 33.0 3 31.285800828063902 33.0 33.0 33.0 27.0 33.0 4 35.32447228284513 37.0 37.0 37.0 33.0 37.0 5 34.7494990702787 37.0 33.0 37.0 27.0 37.0 6 34.61062298090052 37.0 33.0 37.0 27.0 37.0 7 34.06990022065254 37.0 33.0 37.0 27.0 37.0 8 33.73390649273614 37.0 33.0 37.0 27.0 37.0 9 32.54574365694641 37.0 33.0 37.0 22.0 37.0 10-11 33.3692303524186 37.0 33.0 37.0 24.5 37.0 12-13 33.51960993430757 37.0 33.0 37.0 27.0 37.0 14-15 34.26961425849736 37.0 33.0 37.0 27.0 40.0 16-17 33.82293778978732 37.0 33.0 37.0 24.5 40.0 18-19 33.24912255835955 37.0 33.0 37.0 21.0 40.0 20-21 33.24867923668336 37.0 33.0 37.0 18.5 40.0 22-23 33.0817598952423 37.0 33.0 37.0 18.5 40.0 24-25 33.26033323559162 37.0 33.0 37.0 21.0 40.0 26-27 32.91479247536872 37.0 33.0 37.0 18.5 40.0 28-29 33.003374425569305 37.0 33.0 37.0 18.5 40.0 30-31 32.28074955956795 37.0 33.0 37.0 15.0 40.0 32-33 32.779610801604775 37.0 33.0 37.0 18.5 40.0 34-35 32.65355308633616 37.0 33.0 37.0 18.5 40.0 36-37 32.254927670445426 37.0 33.0 37.0 15.0 40.0 38-39 32.09444993723572 37.0 33.0 37.0 15.0 40.0 40-41 32.10973047632405 37.0 33.0 37.0 15.0 40.0 42-43 31.88351768044393 37.0 30.0 37.0 15.0 40.0 44-45 31.33686778808142 35.0 27.0 37.0 15.0 40.0 46-47 29.508949925226354 33.0 24.5 37.0 10.5 40.0 48-49 31.22486225508588 33.0 27.0 37.0 15.0 40.0 50-51 31.57056553106748 33.0 27.0 37.0 15.0 40.0 52-53 31.674175085993994 33.0 27.0 37.0 15.0 40.0 54-55 31.567238569116988 33.0 27.0 37.0 15.0 40.0 56-57 31.342825701869288 33.0 27.0 37.0 15.0 40.0 58-59 30.223425107532968 33.0 27.0 37.0 15.0 40.0 60-61 30.64656740895449 33.0 27.0 37.0 15.0 40.0 62-63 30.683888937755192 33.0 27.0 37.0 15.0 40.0 64-65 29.69544907510294 33.0 27.0 37.0 15.0 37.0 66-67 29.209361571699944 33.0 24.5 37.0 15.0 37.0 68-69 29.47232661986814 33.0 27.0 37.0 15.0 37.0 70-71 29.70245036875297 33.0 27.0 37.0 15.0 37.0 72-73 29.47973090341465 33.0 27.0 37.0 15.0 37.0 74-75 29.594123184239916 33.0 27.0 37.0 15.0 37.0 76-77 28.51688819497825 33.0 27.0 33.0 15.0 37.0 78-79 27.113621468376813 33.0 22.0 33.0 10.5 37.0 80-81 28.43035062334323 33.0 24.5 33.0 15.0 37.0 82-83 28.550551623161493 33.0 27.0 33.0 15.0 37.0 84-85 28.90666140003576 33.0 27.0 33.0 15.0 37.0 86-87 28.407309413691507 33.0 27.0 33.0 15.0 37.0 88-89 28.194231620984233 33.0 27.0 33.0 15.0 37.0 90-91 28.37212911711029 33.0 27.0 33.0 15.0 37.0 92-93 28.486311295595304 33.0 27.0 33.0 15.0 37.0 94-95 28.28156168749806 33.0 27.0 33.0 15.0 37.0 96-97 28.3975180954022 33.0 27.0 33.0 15.0 37.0 98-99 27.85397715204679 33.0 27.0 33.0 15.0 37.0 100-101 27.3034156098907 33.0 24.5 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 1.9182492657465104 2306 2 1.0558574942671726 2306 3 0.858315043026991 2306 4 0.7770155733992823 2306 5 1.0870191125346196 2306 6 1.161058906689739 2306 7 1.340097143236342 2306 8 1.4110431624770712 2306 9 1.9666606092213144 2306 10-11 1.8021286139367945 2306 12-13 1.8809238697217978 2306 14-15 2.172575991604859 2306 16-17 2.3838849728043883 2306 18-19 2.412871056139565 2306 20-21 2.557170134076088 2306 22-23 2.498533032121699 2306 24-25 2.313825345074804 2306 26-27 2.5585324209443883 2306 28-29 2.8437399786024926 2306 30-31 2.795732791242152 2306 32-33 2.3288483043208785 2306 34-35 2.3128514822942705 2306 36-37 2.6302500606461905 2306 38-39 2.476825838997968 2306 40-41 2.6363342826657004 2306 42-43 2.6301866807270713 2306 44-45 2.4929219408838037 2306 46-47 2.563448245340723 2306 48-49 2.439880107589815 2306 50-51 2.471660318020092 2306 52-53 2.521166516596203 2306 54-55 2.917705271895116 2306 56-57 2.6095692204926024 2306 58-59 2.7613034416227293 2306 60-61 2.524341683131677 2306 62-63 2.3359787887397587 2306 64-65 2.4185350034456086 2306 66-67 2.632138199686164 2306 68-69 2.474406782101937 2306 70-71 2.32203575826604 2306 72-73 2.1956354422566626 2306 74-75 2.2268454328795038 2306 76-77 2.198005905985795 2306 78-79 2.7187255369094174 2306 80-81 2.921315668536284 2306 82-83 2.8976217733852216 2306 84-85 2.5626474195097124 2306 86-87 2.5205954991946307 2306 88-89 2.5001784658504285 2306 90-91 2.1726195970464204 2306 92-93 1.8522729333478303 2306 94-95 1.8504709896471923 2306 96-97 1.8113776608929975 2306 98-99 2.1300382243010567 2306 100-101 1.9149610213351878 2307 1 1.3987083002173613 2307 2 0.945442871237578 2307 3 0.6535595458252175 2307 4 0.6766971933547836 2307 5 0.9953945236846593 2307 6 0.9237297580763268 2307 7 1.2612939102076908 2307 8 1.3953087738264003 2307 9 1.9013700854421742 2307 10-11 1.811825739436216 2307 12-13 1.896148430448477 2307 14-15 1.9974710377662035 2307 16-17 2.3011040947219 2307 18-19 2.204131090496375 2307 20-21 2.345489225998776 2307 22-23 2.321524165991228 2307 24-25 2.120997425700523 2307 26-27 2.286997762939123 2307 28-29 2.6030596888878463 2307 30-31 2.731563829629799 2307 32-33 2.342944557474766 2307 34-35 2.4214142067408275 2307 36-37 2.5530956621860597 2307 38-39 2.2292656874442116 2307 40-41 2.370764825040638 2307 42-43 2.421748995052944 2307 44-45 2.2915078526830257 2307 46-47 2.5794768974548177 2307 48-49 2.377661387121435 2307 50-51 2.375246194571652 2307 52-53 2.509819729923585 2307 54-55 2.8063202461786645 2307 56-57 2.5331222474627424 2307 58-59 2.7183826883057662 2307 60-61 2.397966119387295 2307 62-63 2.345720644469104 2307 64-65 2.510744500877461 2307 66-67 2.6677504810476727 2307 68-69 2.3978758151204573 2307 70-71 2.1642354534142356 2307 72-73 2.1174723521576233 2307 74-75 2.152424280733591 2307 76-77 2.2644152001914293 2307 78-79 2.736016617102674 2307 80-81 3.076014505887187 2307 82-83 2.9106036620714555 2307 84-85 2.4917593862623626 2307 86-87 2.66210940723926 2307 88-89 2.6157302653282386 2307 90-91 2.3270372640399053 2307 92-93 2.2676042695626926 2307 94-95 2.195250806494059 2307 96-97 2.095087511612565 2307 98-99 2.2055624959718862 2307 100-101 2.1889969694675813 2201 1 1.862103580825675 2201 2 1.1130280770099397 2201 3 0.8540981571275879 2201 4 0.6821403324472328 2201 5 1.020221373691939 2201 6 0.998436451115154 2201 7 1.2165587626398136 2201 8 1.2709829521000273 2201 9 1.8374343718884916 2201 10-11 1.5974817002826 2201 12-13 1.6948723778371715 2201 14-15 1.9890563601503075 2201 16-17 2.24822329429729 2201 18-19 1.9548648087393303 2201 20-21 2.1429803814732367 2201 22-23 2.1204391698241594 2201 24-25 2.0297765831660257 2201 26-27 2.1202213849418143 2201 28-29 2.4882643094254817 2201 30-31 2.5342049161294895 2201 32-33 2.0591843768422464 2201 34-35 2.1358521618960395 2201 36-37 2.315596836863243 2201 38-39 2.123193301999706 2201 40-41 2.2064337202171487 2201 42-43 2.3239213392345732 2201 44-45 2.2275999707096688 2201 46-47 2.362862328226683 2201 48-49 2.172267598598296 2201 50-51 2.1733466032014164 2201 52-53 2.446595230008633 2201 54-55 2.8468531242918402 2201 56-57 2.499036273625194 2201 58-59 2.7619976168430114 2201 60-61 2.410651261724592 2201 62-63 2.2035030889697467 2201 64-65 2.2602133074516466 2201 66-67 2.3828588706344824 2201 68-69 2.2354944167947153 2201 70-71 2.1023261598005263 2201 72-73 2.0535242311541246 2201 74-75 2.0644349431291893 2201 76-77 2.15519716471573 2201 78-79 2.575923765831483 2201 80-81 2.917185918072377 2201 82-83 2.759311598614321 2201 84-85 2.5042705500585534 2201 86-87 2.4490612111195773 2201 88-89 2.423598677749414 2201 90-91 2.1526622080900353 2201 92-93 2.1181871770398857 2201 94-95 2.16772563117172 2201 96-97 2.1151343437936703 2201 98-99 2.210262002496112 2201 100-101 2.031605917264983 1101 1 2.1865828318316147 1101 2 1.2450465315529478 1101 3 0.6699335618158742 1101 4 0.6066168775497758 1101 5 0.7506002974104078 1101 6 0.8496548898173515 1101 7 1.0397038059673847 1101 8 1.2236303121459855 1101 9 1.5756850433964686 1101 10-11 1.4344303742278584 1101 12-13 1.539709905250291 1101 14-15 1.8302030952312336 1101 16-17 1.9534303755337206 1101 18-19 1.8688524724975721 1101 20-21 2.0745002556994763 1101 22-23 2.13644219254374 1101 24-25 1.8696259291759532 1101 26-27 2.1130745585366526 1101 28-29 2.5041059820852993 1101 30-31 2.5769563752385665 1101 32-33 2.0961464886983734 1101 34-35 2.058708864439886 1101 36-37 2.166067417421857 1101 38-39 1.9476337084940525 1101 40-41 2.211491416560392 1101 42-43 2.1618933238329063 1101 44-45 2.111597804154833 1101 46-47 2.400435153318071 1101 48-49 2.272585936857922 1101 50-51 2.1539060790284665 1101 52-53 2.320326467845284 1101 54-55 2.6611078832641937 1101 56-57 2.2191022701861627 1101 58-59 2.684645157987191 1101 60-61 2.278297669771721 1101 62-63 2.154713924365275 1101 64-65 2.2495487537854792 1101 66-67 2.2258324252205206 1101 68-69 2.2629958025132098 1101 70-71 2.091128247207692 1101 72-73 1.9636908633627996 1101 74-75 1.9952781829580601 1101 76-77 2.041068660095185 1101 78-79 2.443013508244981 1101 80-81 2.622292954872801 1101 82-83 2.6586257084771 1101 84-85 2.334523275798187 1101 86-87 2.4727199145380503 1101 88-89 2.6533293297297895 1101 90-91 2.233127354292673 1101 92-93 2.0835547317361254 1101 94-95 2.1844949741882402 1101 96-97 1.9722975077952327 1101 98-99 2.160351596789585 1101 100-101 2.1681514397911315 2304 1 -1.073868224957156 2304 2 0.09604136453317835 2304 3 0.014628562309660254 2304 4 0.2879708864155148 2304 5 0.604576182167051 2304 6 0.7302394357140187 2304 7 0.8313005510989626 2304 8 1.1443059377131348 2304 9 1.6167133790869173 2304 10-11 1.2079533109136946 2304 12-13 1.3071276344820575 2304 14-15 1.5406964496574886 2304 16-17 1.7333395567297174 2304 18-19 1.6618012979462122 2304 20-21 1.8100734539039678 2304 22-23 1.7741231453772102 2304 24-25 1.587232646860656 2304 26-27 1.7737492617544461 2304 28-29 2.0480443766011476 2304 30-31 2.2213938166600613 2304 32-33 1.5732811684754395 2304 34-35 1.6044079214075353 2304 36-37 1.8251238633395843 2304 38-39 1.6622794983884503 2304 40-41 1.6053266756473903 2304 42-43 1.6055878081837136 2304 44-45 1.7061648403202412 2304 46-47 1.8270720905219235 2304 48-49 1.585272898937255 2304 50-51 1.4939229980834554 2304 52-53 1.7273602202419909 2304 54-55 2.083524419963453 2304 56-57 1.675349789625372 2304 58-59 1.7332787765966557 2304 60-61 1.3648923931880326 2304 62-63 1.225784389993315 2304 64-65 1.2955706796136859 2304 66-67 1.4001827745021096 2304 68-69 1.3103342655996677 2304 70-71 1.19756545384935 2304 72-73 1.079413892097243 2304 74-75 1.2458082543423075 2304 76-77 1.261806288036226 2304 78-79 1.668438021868731 2304 80-81 2.158529173203142 2304 82-83 2.004016104491061 2304 84-85 1.478791386919859 2304 86-87 1.4586980613797955 2304 88-89 1.479313634883951 2304 90-91 1.2208236796155418 2304 92-93 1.1505796239889534 2304 94-95 0.9141358355693825 2304 96-97 0.8103683628596734 2304 98-99 1.2866528547250518 2304 100-101 1.0467381676496217 2202 1 1.9366491545095101 2202 2 0.4459782580749234 2202 3 0.41488010517166884 2202 4 0.2357120907256558 2202 5 0.3164316782461398 2202 6 0.17205439822929947 2202 7 -4.6700481092262365 2202 8 -1.0506758782777794 2202 9 0.6667911844157004 2202 10-11 0.8011684317404928 2202 12-13 0.9883187416134547 2202 14-15 0.9721521364583197 2202 16-17 0.921390985117128 2202 18-19 1.2350551188734613 2202 20-21 1.4646632930889751 2202 22-23 1.3061423235077925 2202 24-25 1.498175202843612 2202 26-27 1.4581704067289394 2202 28-29 1.3312645771082785 2202 30-31 1.6267290392051237 2202 32-33 1.2831632243228412 2202 34-35 1.455268039060826 2202 36-37 1.5312912081626209 2202 38-39 1.1903193598554935 2202 40-41 1.360623333134626 2202 42-43 1.3612447438172026 2202 44-45 1.307910525968012 2202 46-47 1.5239118333592074 2202 48-49 1.2437192439046783 2202 50-51 1.0691406244703714 2202 52-53 1.2060140582053833 2202 54-55 1.5195656090033935 2202 56-57 1.3748118525157622 2202 58-59 1.3680275038199028 2202 60-61 1.258323348489963 2202 62-63 1.1720786644975831 2202 64-65 1.0231314773624192 2202 66-67 1.0398950194403476 2202 68-69 1.0711747345507732 2202 70-71 0.7881509571272503 2202 72-73 0.7395117045105692 2202 74-75 0.7981594243827885 2202 76-77 0.8057921127155723 2202 78-79 1.3957133343106278 2202 80-81 1.6368895012113 2202 82-83 1.6319468151580772 2202 84-85 1.13274709709345 2202 86-87 1.046704640033294 2202 88-89 1.1678024455635345 2202 90-91 0.5240714028487936 2202 92-93 0.550567080606509 2202 94-95 0.4793833427344296 2202 96-97 0.44553161900749316 2202 98-99 0.6320840510261299 2202 100-101 0.5736759774036457 1102 1 2.1472086157362185 1102 2 1.2921899138978254 1102 3 0.7237197101480248 1102 4 0.043522911873211 1102 5 0.3170069235687336 1102 6 0.6917321674845169 1102 7 0.9572050924132824 1102 8 -3.3880126063606326 1102 9 0.3006966916880387 1102 10-11 0.9127055877200618 1102 12-13 0.37073409063248164 1102 14-15 0.5408267358900645 1102 16-17 0.9587218001382212 1102 18-19 1.651896213452332 1102 20-21 1.7735623065524067 1102 22-23 -2.0141546166716964 1102 24-25 -2.411460903778668 1102 26-27 -0.8019327873136319 1102 28-29 1.4058235343909686 1102 30-31 2.068989158126577 1102 32-33 1.894876271660344 1102 34-35 -1.1746366205985694 1102 36-37 0.5690689483971454 1102 38-39 1.1717890802554791 1102 40-41 2.013367110498514 1102 42-43 2.1475944851929967 1102 44-45 2.2640008462081056 1102 46-47 1.7741938152707917 1102 48-49 1.8929973224174717 1102 50-51 -3.108943651331284 1102 52-53 0.5091771238878309 1102 54-55 2.211989559748922 1102 56-57 2.294037344792045 1102 58-59 2.5699374727863287 1102 60-61 2.4148973894152412 1102 62-63 2.2670220963919476 1102 64-65 2.2678156126065936 1102 66-67 2.3286672266273634 1102 68-69 2.2398799996543417 1102 70-71 2.23449775753285 1102 72-73 2.0610419960034 1102 74-75 1.416028530313401 1102 76-77 2.0373364966351133 1102 78-79 2.553855475868211 1102 80-81 2.8914188565063945 1102 82-83 2.882584275449144 1102 84-85 2.5703326461484686 1102 86-87 2.6459461842716117 1102 88-89 2.6676264986317086 1102 90-91 2.2912021374211236 1102 92-93 2.2085832917321575 1102 94-95 2.1219018302221357 1102 96-97 1.9989130067119483 1102 98-99 2.3305443264214247 1102 100-101 2.178901412327683 2305 1 2.014776690472619 2305 2 1.0702655124838394 2305 3 0.38291747049046165 2305 4 0.10858252956934678 2305 5 0.5720976307142323 2305 6 0.6455519095384474 2305 7 0.8085412286996885 2305 8 0.39978795456809024 2305 9 1.2611704888103645 2305 10-11 0.709798749562708 2305 12-13 1.1498227589592815 2305 14-15 1.0283237562731742 2305 16-17 1.4032185111483528 2305 18-19 1.5905959847191866 2305 20-21 1.5410253973407038 2305 22-23 1.4868559172249292 2305 24-25 1.4533177423848542 2305 26-27 1.6593689629260098 2305 28-29 -1.6369181188779898 2305 30-31 -0.28833917790320385 2305 32-33 0.6599784915383182 2305 34-35 0.040318013149459375 2305 36-37 0.7588134874567487 2305 38-39 -2.325373310132658 2305 40-41 -3.856705110343082 2305 42-43 -3.730354067600743 2305 44-45 -1.048573644214013 2305 46-47 0.5676011276310078 2305 48-49 1.1053552517485628 2305 50-51 1.4428002486955975 2305 52-53 1.2117555702268348 2305 54-55 1.6239120846382349 2305 56-57 1.1381691478279592 2305 58-59 -3.5145467111536632 2305 60-61 0.04841465597811023 2305 62-63 1.2349766337778973 2305 64-65 1.6638802360590965 2305 66-67 1.6332208419803784 2305 68-69 1.3414240751616333 2305 70-71 1.5208604948244648 2305 72-73 1.0780752612070508 2305 74-75 1.3650162213258668 2305 76-77 0.9703496373795382 2305 78-79 1.7488859820734248 2305 80-81 1.845185345833027 2305 82-83 2.045045667221853 2305 84-85 2.035732320546078 2305 86-87 2.2344471453565475 2305 88-89 1.9426717634499155 2305 90-91 1.7830037532610206 2305 92-93 1.5820953851729413 2305 94-95 1.2916284155051123 2305 96-97 1.5284923608831598 2305 98-99 1.861731405788273 2305 100-101 1.7242537171564365 2203 1 1.76937624707627 2203 2 0.8607607045961778 2203 3 0.33623267285201663 2203 4 -0.29613022342122264 2203 5 0.09984767631233638 2203 6 0.32999028621704696 2203 7 0.4685477340728994 2203 8 0.19487540495755695 2203 9 0.4171814891637098 2203 10-11 0.7101789857996721 2203 12-13 0.17267480557441672 2203 14-15 0.2630269734412636 2203 16-17 0.5731304893853988 2203 18-19 0.9668115670276762 2203 20-21 0.7899101670219579 2203 22-23 0.8821160552251044 2203 24-25 0.8830391152908064 2203 26-27 0.9822591416534721 2203 28-29 0.7079318531713952 2203 30-31 0.47183046479307933 2203 32-33 0.2218823546937756 2203 34-35 0.6516215692229039 2203 36-37 1.2196759155797423 2203 38-39 0.4296818329980425 2203 40-41 -2.939886077412112 2203 42-43 -0.5483216565873938 2203 44-45 0.515586207284116 2203 46-47 -2.4002829481174714 2203 48-49 -0.8244001635768399 2203 50-51 -3.1995001220199413 2203 52-53 -1.3079202578340663 2203 54-55 -4.22138285520186 2203 56-57 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88-89 0.25158869931022565 1208 90-91 1.1393765038620742 1208 92-93 1.3434185927446372 1208 94-95 -1.8084096514120063 1208 96-97 -5.150203772489373 1208 98-99 -6.783975299248002 1208 100-101 -7.577013578792581 1209 1 1.9199611363745852 1209 2 1.066325444267889 1209 3 0.06862250256509483 1209 4 -0.28240120760399634 1209 5 -0.6064162567827793 1209 6 -0.2535303991423632 1209 7 -0.46510332542400334 1209 8 -1.0628469754233834 1209 9 -2.3950878371458764 1209 10-11 -2.288535610385562 1209 12-13 -2.6407117316593087 1209 14-15 -2.325047884422929 1209 16-17 -1.9820894252838173 1209 18-19 -1.2409494405364683 1209 20-21 -2.9007487981323408 1209 22-23 -2.095574626809878 1209 24-25 -1.3212415515180922 1209 26-27 -1.8487488543114736 1209 28-29 -0.7012734640112157 1209 30-31 -1.5497988353154852 1209 32-33 -2.0112837157721444 1209 34-35 -6.092510312932447 1209 36-37 -3.682256192857153 1209 38-39 -1.0088688056814306 1209 40-41 -1.3970204530812858 1209 42-43 -0.5286658131562447 1209 44-45 -0.5682741172946493 1209 46-47 -0.6312961791600813 1209 48-49 -2.269809049761321 1209 50-51 -1.0057124338972763 1209 52-53 -2.3791122860037106 1209 54-55 -1.8802459698502574 1209 56-57 -7.27087796096707 1209 58-59 -2.67482462266301 1209 60-61 -1.8323429457156415 1209 62-63 -0.2604251151599968 1209 64-65 -0.3971837196401431 1209 66-67 0.37586493388974773 1209 68-69 -0.2869420135769687 1209 70-71 -4.9728859893317825 1209 72-73 -1.7565763562315553 1209 74-75 -6.905911675246266 1209 76-77 -2.538717319069569 1209 78-79 -4.7112177780194955 1209 80-81 -0.041973744704062455 1209 82-83 -0.42331651745837817 1209 84-85 -3.700886854663775 1209 86-87 -1.8085513267067306 1209 88-89 -5.92220256694916 1209 90-91 -0.9687350416848872 1209 92-93 -3.175866380610376 1209 94-95 -5.755230422635712 1209 96-97 -4.657712584968493 1209 98-99 -7.447937538087373 1209 100-101 -5.000309605413722 1315 1 1.9637501777317325 1315 2 1.2885387425381545 1315 3 0.2072460978820807 1315 4 -0.0861345384302652 1315 5 -0.20678882431568013 1315 6 0.5649278105725983 1315 7 0.4026576296597142 1315 8 -0.3786776035905106 1315 9 0.11951912022374955 1315 10-11 -2.8618935398018763 1315 12-13 -0.8942056603127639 1315 14-15 -2.393390724451244 1315 16-17 -0.6897996526252257 1315 18-19 -16.910441478147796 1315 20-21 -14.09890409190869 1315 22-23 -3.6401124295210003 1315 24-25 -2.7191460270292254 1315 26-27 -2.8197826687771403 1315 28-29 -3.872152624001707 1315 30-31 -1.7987934804966201 1315 32-33 -2.3241009656220584 1315 34-35 -3.690315468439774 1315 36-37 -5.962300057513332 1315 38-39 -2.875098795621838 1315 40-41 -11.341475522699302 1315 42-43 -6.120944304029415 1315 44-45 -3.9078576716748685 1315 46-47 -3.1961534175670394 1315 48-49 -4.673551043387114 1315 50-51 -3.0759890928233844 1315 52-53 -2.312445999873461 1315 54-55 -5.304668550612398 1315 56-57 -2.510073569666396 1315 58-59 -3.894269719988557 1315 60-61 -4.27034020048119 1315 62-63 -2.6772992413252794 1315 64-65 -1.520411102682278 1315 66-67 -1.2685225793227026 1315 68-69 -1.7633138585383783 1315 70-71 -1.991896603611572 1315 72-73 -1.9207752218748944 1315 74-75 -1.2666214472870436 1315 76-77 -17.275964066490538 1315 78-79 -16.16084883677477 1315 80-81 -21.440616304165115 1315 82-83 -11.506338504852327 1315 84-85 -6.374170848093481 1315 86-87 -4.786870354109382 1315 88-89 -4.7568248890360785 1315 90-91 -8.382362766941178 1315 92-93 -4.934979377092876 1315 94-95 -2.457382691819113 1315 96-97 -1.4550867494112616 1315 98-99 -0.045265702393148644 1315 100-101 -1.357576149681968 1212 1 -27.966082757829128 1212 2 -15.91275554789695 1212 3 -6.275234064490686 1212 4 -2.024558934462057 1212 5 -1.174582597986543 1212 6 -2.6514330472560452 1212 7 -0.9631687851523765 1212 8 -1.917791726893178 1212 9 0.015286413127078902 1212 10-11 0.3927959256871105 1212 12-13 -0.48095528973840374 1212 14-15 -1.1058286787448068 1212 16-17 -0.3752035701741363 1212 18-19 -1.6616303333790157 1212 20-21 -1.3044779636967618 1212 22-23 -1.2765419710967265 1212 24-25 -0.5361005262778775 1212 26-27 -2.0495418294432213 1212 28-29 -1.051446696851869 1212 30-31 -1.3109078621814056 1212 32-33 -0.9298019036171468 1212 34-35 -2.852171340513422 1212 36-37 -3.535905856492043 1212 38-39 -2.7748531626196424 1212 40-41 -2.3299826605053653 1212 42-43 -1.9496672589009378 1212 44-45 -1.4154196833836572 1212 46-47 -3.4688823747232043 1212 48-49 -4.45643301545465 1212 50-51 -3.309475288078673 1212 52-53 -4.964731024249772 1212 54-55 -4.2019925532914115 1212 56-57 -5.115378690052069 1212 58-59 -4.068475109975566 1212 60-61 -4.395872943615366 1212 62-63 -7.447487724282254 1212 64-65 -8.010658495062724 1212 66-67 -5.835419410062013 1212 68-69 -4.330582751031017 1212 70-71 -6.080247012263293 1212 72-73 -6.984333101169643 1212 74-75 -6.470464456350818 1212 76-77 -5.157024487109432 1212 78-79 -6.989595377514149 1212 80-81 -5.544215210120825 1212 82-83 -5.583136087959577 1212 84-85 -8.187992591628824 1212 86-87 -8.047584840166948 1212 88-89 -7.500760473693621 1212 90-91 -7.125703605255168 1212 92-93 -8.555589600865392 1212 94-95 -8.718243537748574 1212 96-97 -6.802037245436935 1212 98-99 -7.579624107291529 1212 100-101 -7.096789716103174 1213 1 2.1285075110569167 1213 2 1.532290251374203 1213 3 -0.7259853476363318 1213 4 -0.6239338227453999 1213 5 -0.7136477256080056 1213 6 0.40849550879926966 1213 7 -0.498828287361178 1213 8 -0.12138362273859826 1213 9 -0.42100554217896047 1213 10-11 -0.2774223276297363 1213 12-13 0.3597795003801849 1213 14-15 0.20445171639474324 1213 16-17 -16.209717931008917 1213 18-19 -14.1558189010711 1213 20-21 -1.7330286155748809 1213 22-23 -0.5184589514820104 1213 24-25 -2.143539054755145 1213 26-27 -1.20362486820936 1213 28-29 -16.750130264197406 1213 30-31 -15.27283642006067 1213 32-33 -5.21057786018536 1213 34-35 -2.4393053886509932 1213 36-37 -1.907297750331118 1213 38-39 -1.3476294994804832 1213 40-41 -4.040586646373434 1213 42-43 -6.27635858075071 1213 44-45 -3.687153270610203 1213 46-47 -1.977325053588416 1213 48-49 -3.6727472730541137 1213 50-51 -3.323853153953017 1213 52-53 -1.0468833300816556 1213 54-55 -3.6531081467485507 1213 56-57 -1.5176003696505092 1213 58-59 -0.2721156139681824 1213 60-61 -1.3177588302899856 1213 62-63 -1.546077221639358 1213 64-65 0.8676086244896091 1213 66-67 -0.8125405218662252 1213 68-69 -1.4475787994531224 1213 70-71 -0.4683155588705681 1213 72-73 0.3492241898700392 1213 74-75 0.2727930555686662 1213 76-77 0.9696307434190388 1213 78-79 -13.58470319093182 1213 80-81 -11.874937702286978 1213 82-83 -20.912545818161558 1213 84-85 -7.501040276859609 1213 86-87 -13.985726618185486 1213 88-89 -20.6349295388486 1213 90-91 -13.186837401688262 1213 92-93 -4.8325169184617245 1213 94-95 -0.5803069535972014 1213 96-97 1.1925830729509883 1213 98-99 1.983463681940627 1213 100-101 1.9257814268905022 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 7.0 9 20.0 10 62.0 11 140.0 12 605.0 13 2421.0 14 7102.0 15 16052.0 16 29869.0 17 50494.0 18 77085.0 19 108900.0 20 145621.0 21 187902.0 22 235891.0 23 289797.0 24 352123.0 25 418440.0 26 497539.0 27 582727.0 28 677616.0 29 778827.0 30 883219.0 31 979973.0 32 1063635.0 33 1110332.0 34 1107354.0 35 1048458.0 36 904363.0 37 547261.0 38 94774.0 39 208.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 31.51196022969251 27.09543170270653 20.14599869964688 21.246609367954083 2 23.9013166329636 28.95999013664744 27.115169396850085 20.023523833538874 3 19.732199244902898 26.577062007283402 31.446484116007156 22.244254631806548 4 22.659668172687265 26.735646044308403 29.419259165746837 21.185426617257498 5 21.23869880169528 28.351822967751712 28.35808587012987 22.051392360423147 6 21.142730479521088 28.637383444640573 27.87841640709915 22.34146966873919 7 22.07738668429898 28.215350718024546 28.771683352574268 20.93557924510221 8 20.27795500364011 27.450909346969855 29.945808644106158 22.325327005283878 9 21.385101648772373 27.288151364472196 28.996238007992403 22.330508978763024 10-11 21.765569563015823 27.771963461702885 28.154098876964873 22.308368098316418 12-13 21.61693308482978 27.629042702509032 28.34715963066323 22.406864581997958 14-15 21.64106748648968 27.70664537592662 28.218186310452552 22.43410082713115 16-17 21.988138366650173 27.744834332459984 27.843805149729484 22.42322215116036 18-19 22.076953576460067 27.736209180101064 27.835577320544292 22.351259922894577 20-21 21.9130431859894 27.534285902602228 28.030846573147684 22.521824338260686 22-23 21.881640782001462 27.54248900677428 28.09421499819487 22.481655213029388 24-25 21.68345704248904 27.712274655887686 28.036526300956538 22.567742000666737 26-27 22.03453417296729 27.741358453874927 27.78962577944086 22.434481593716917 28-29 22.386574488305886 27.762724728349664 27.546782898536403 22.30391788480805 30-31 21.994992341338982 27.848358265604055 27.32715808558181 22.82949130747515 32-33 22.226494535217093 27.683752052114073 27.662507985994083 22.42724542667475 34-35 22.422542849992258 27.575875024192996 27.595475349701037 22.406106776113706 36-37 22.362793257629125 27.602825795743897 27.573683147851924 22.46069779877506 38-39 22.127508127544033 27.586501021999005 27.87692679234295 22.40906405811401 40-41 22.43527228634355 27.552007011940983 27.516412820319548 22.496307881395925 42-43 22.178874802377887 27.550027685847994 27.789500331209556 22.481597180564563 44-45 22.12984488466323 27.393608343735075 27.92839284364564 22.54815392795606 46-47 22.211712593639124 27.567814522641186 27.81646770110924 22.404005182610444 48-49 22.33377889783511 27.557212806106644 27.741241288241188 22.36776700781706 50-51 22.34122127482871 27.547452028442876 27.61399678724282 22.497329909485604 52-53 22.261928590762007 27.626141562080818 27.830230966422846 22.28169888073432 54-55 22.239415464030195 27.51807182885326 27.79522335231759 22.44728935479896 56-57 22.20861440623018 27.619034577659964 27.802502302981186 22.369848713128675 58-59 21.944464987506194 27.335384139326173 27.995880832412794 22.72427004075484 60-61 22.383991403265664 27.529817380078953 27.569561039421544 22.51663017723384 62-63 22.003256637179753 27.348749318151327 27.822603394171345 22.825390650497575 64-65 21.86707670637987 27.265798531867354 28.066711846371074 22.800412915381703 66-67 22.070871526538628 27.521775727022906 28.028560254760993 22.378792491677473 68-69 22.168620854019462 27.568453937986675 27.900137310909912 22.36278789708395 70-71 22.508815765570144 27.579930359655346 27.446993658882057 22.464260215892452 72-73 22.139463859881563 27.524084868643072 27.88640531651418 22.45004595496118 74-75 22.332567492586865 27.580750425494426 27.679339296653694 22.407342785265012 76-77 22.375654676705466 27.75808298421142 27.510120263540788 22.356142075542333 78-79 22.38071716258958 27.66801716131857 27.45160284065865 22.499662835433195 80-81 22.427509791704576 27.686601444028952 27.450279313104563 22.435609451161906 82-83 22.38810409650863 27.734395300808877 27.42369653267403 22.453804070008463 84-85 22.37371890077032 27.592099317430495 27.498234734037364 22.535947047761823 86-87 22.30164512142151 27.702634482823953 27.468523510774588 22.527196884979944 88-89 22.411852776983302 27.732759110374378 27.37482271783086 22.480565394811457 90-91 22.483388879784407 27.645929165404404 27.285576307163634 22.585105647647556 92-93 22.37993023813751 27.603745991466184 27.3921523484076 22.624171421988706 94-95 22.409774568890835 27.64487245042289 27.30785753939113 22.637495441295144 96-97 22.4335792308957 27.70585804880582 27.25582876974797 22.604733950550514 98-99 22.31268496864959 27.644505619312437 27.377007838987527 22.665801573050445 100-101 22.482680664610825 27.593104579530987 27.125997305276677 22.79821745058151 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 61.0 1 45.0 2 30.5 3 40.5 4 83.0 5 131.0 6 196.0 7 335.0 8 505.0 9 756.5 10 1118.5 11 1689.5 12 2570.0 13 3637.5 14 5119.0 15 7287.5 16 10120.5 17 13811.5 18 18734.5 19 25379.5 20 34027.5 21 44554.5 22 57438.5 23 72376.0 24 89332.5 25 108783.0 26 129770.0 27 151577.0 28 174372.5 29 197449.0 30 220088.0 31 241810.0 32 262394.5 33 281559.0 34 303301.5 35 323947.0 36 343497.0 37 366937.5 38 389527.5 39 402159.0 40 410839.5 41 428562.0 42 437803.0 43 436114.0 44 438997.5 45 440051.0 46 429931.0 47 416730.0 48 386421.5 49 333519.0 50 286624.0 51 250025.5 52 220494.0 53 203809.0 54 194360.5 55 182276.0 56 171323.5 57 164429.5 58 160095.5 59 158345.5 60 156381.0 61 152269.0 62 148133.0 63 144553.0 64 139644.0 65 133343.5 66 126427.5 67 118550.5 68 109438.5 69 99900.0 70 89371.0 71 77682.0 72 66043.5 73 54461.0 74 42994.0 75 32748.0 76 23881.0 77 16509.5 78 11119.5 79 7123.5 80 4366.0 81 2623.0 82 1520.0 83 902.0 84 538.0 85 341.5 86 214.5 87 146.0 88 108.5 89 73.0 90 37.0 91 24.0 92 18.0 93 11.0 94 11.0 95 5.5 96 1.5 97 2.5 98 4.0 99 5.0 100 10.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 5.779380082511279 2 0.0010246895252219949 3 9.91899460414891E-4 4 1.639503240355192E-5 5 0.0 6 0.0 7 0.0 8 6.558012961420768E-5 9 4.237902740896924 10-11 0.0 12-13 0.24573694318063793 14-15 7.090851514536204E-4 16-17 1.2860550330413187 18-19 2.8274626957679585 20-21 0.004172535746703963 22-23 1.784505005690306 24-25 0.20190892280784276 26-27 7.254801838571724E-4 28-29 1.2806323760738438 30-31 0.13016016225179866 32-33 9.550106375068993E-4 34-35 3.2790064807103835E-4 36-37 0.001639503240355192 38-39 0.0014345653353107929 40-41 0.001619009449850752 42-43 0.00284043936391537 44-45 0.004418461232757242 46-47 4.077264213406924 48-49 0.0035536232734698784 50-51 0.002135452970562637 52-53 0.010013266040469334 54-55 0.012443829594295906 56-57 0.026326323282003492 58-59 0.07357680666904012 60-61 0.036224824095647964 62-63 0.033027792776955334 64-65 0.03730689623428239 66-67 0.5503197564157246 68-69 0.2346620987920386 70-71 0.05059506999736122 72-73 0.21337314921602643 74-75 0.06798200186132804 76-77 0.13891101079719453 78-79 5.513210830197715 80-81 0.1075473138091997 82-83 1.3365517328442587 84-85 0.0425205165386119 86-87 1.3525409881958226 88-89 1.3615459597434736 90-91 0.08254488939378302 92-93 0.0907383068374581 94-95 0.09810377514475378 96-97 0.1525516777569497 98-99 0.12431123444183152 100-101 0.17472595908275368 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.2198817E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.61629927841583 #Duplication Level Percentage of deduplicated Percentage of total 1 96.93520508445779 46.15695735916158 2 1.886411344995928 1.796478543310501 3 0.23801541814647825 0.34000240150019984 4 0.10204504439507936 0.19436029495181317 5 0.020416698132957847 0.04860838042879974 6 0.01702032443098038 0.04862669171527766 7 0.01094575844552736 0.03648375569800349 8 0.003193715230684538 0.012165832018744787 9 0.00851978102143964 0.03651123986130936 >10 0.022832711096623517 0.29487322215948264 >50 0.16890788444916477 6.622617984757681 >100 0.5864862351973776 44.412314294436804 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.1313542124617494E-4 8.19751620177596E-6 0.0 2 2.4592548605327876E-4 8.19751620177596E-6 0.0 3 2.951105832639345E-4 1.639503240355192E-5 2.4592548605327878E-5 4 3.2790064807103835E-4 1.639503240355192E-5 4.9185097210655755E-5 5 3.770857452816941E-4 1.639503240355192E-5 4.9185097210655755E-5 6 4.180733262905739E-4 1.639503240355192E-5 5.738261341243172E-5 7 4.5086339109767776E-4 1.639503240355192E-5 6.558012961420768E-5 8 5.164435207118854E-4 2.4592548605327878E-5 6.558012961420768E-5 9 5.410360693172133E-4 3.279006480710384E-5 8.197516201775959E-5 10-11 5.943199246287571E-4 3.279006480710384E-5 9.017267821953555E-5 12-13 6.803938447474047E-4 4.0987581008879794E-5 9.017267821953555E-5 14-15 7.582702486642763E-4 5.738261341243172E-5 9.017267821953555E-5 16-17 8.1155410397582E-4 5.738261341243172E-5 9.837019442131151E-5 18-19 8.361466525811478E-4 6.14813715133197E-5 1.0656771062308747E-4 20-21 9.181218145989075E-4 6.558012961420768E-5 1.1066646872397545E-4 22-23 9.345168470024593E-4 6.558012961420768E-5 1.1886398492575141E-4 24-25 9.87800702314003E-4 6.558012961420768E-5 1.3525901732930334E-4 26-27 0.0011066646872397545 7.787640391687161E-5 1.393577754301913E-4 28-29 0.0013279976246877052 9.427143632042352E-5 1.4755529163196725E-4 30-31 0.001778861015785383 1.1886398492575141E-4 1.5575280783374323E-4 32-33 0.002549427538752323 1.2296274302663938E-4 1.7214784023729515E-4 34-35 0.003807746275724933 1.3116025922841536E-4 1.8444411453995909E-4 36-37 0.0056234961144183086 1.3116025922841536E-4 1.9674038884262302E-4 38-39 0.008185219927473295 1.4755529163196725E-4 2.0903666314528696E-4 40-41 0.01202985502610622 1.4755529163196725E-4 2.213329374479509E-4 42-43 0.01745251199358102 1.5575280783374323E-4 2.3362921175061483E-4 44-45 0.025342621337790378 1.5575280783374323E-4 2.3772796985150281E-4 46-47 0.03650353964650835 1.5985156593463121E-4 2.4592548605327876E-4 48-49 0.05181649991142584 1.6395032403551917E-4 2.664192765577187E-4 50-51 0.07296609171200782 1.803453564390711E-4 2.787155508603826E-4 52-53 0.09615686504683199 1.803453564390711E-4 2.992093413648225E-4 54-55 0.12354476667696548 1.8854287264084705E-4 3.1150561566748645E-4 56-57 0.15793334714341561 1.9264163074173503E-4 3.2790064807103835E-4 58-59 0.19686335158564966 1.9674038884262302E-4 3.319994061719263E-4 60-61 0.24354410759666284 2.1313542124617494E-4 3.360981642728143E-4 62-63 0.29684845669871107 2.213329374479509E-4 3.442956804745903E-4 64-65 0.357075608233159 2.213329374479509E-4 3.524931966763662E-4 66-67 0.4300130086384606 2.213329374479509E-4 3.811845033825821E-4 68-69 0.5061843291853628 2.213329374479509E-4 4.01678293887022E-4 70-71 0.5985539417469743 2.2543169554883888E-4 4.09875810088798E-4 72-73 0.6955961385435981 2.3362921175061483E-4 4.3036960059323785E-4 74-75 0.8049510046752895 2.3772796985150281E-4 4.344683586941258E-4 76-77 0.92644229354371 2.4592548605327876E-4 4.426658748959018E-4 78-79 1.066033698185652 2.4592548605327876E-4 4.4676463299678974E-4 80-81 1.2390832652051424 2.5412300225505474E-4 4.6725842350122965E-4 82-83 1.4228592821746568 2.5412300225505474E-4 4.6725842350122965E-4 84-85 1.6155091104325936 2.5412300225505474E-4 4.6725842350122965E-4 86-87 1.822451308188327 2.664192765577187E-4 4.959497302074456E-4 88-89 2.0479403863505783 2.787155508603826E-4 5.041472464092215E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGTCAC 11355 0.0 5.5293784 94-95 CGTCTGA 18880 0.0 5.4329247 94-95 ACGTCTG 20075 0.0 5.304277 94-95 CACGTCT 21405 0.0 5.1895857 94-95 CACACGT 23675 0.0 5.1097136 94-95 CCAGTCA 12240 0.0 5.073213 94-95 GCACACG 25340 0.0 5.0553284 94-95 ACACGTC 22200 0.0 5.0348215 94-95 >>END_MODULE