FastQCFastQC Report
Thu 11 Sep 2014
lane7_Undetermined_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane7_Undetermined_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12198817
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTCAC113550.05.529378494-95
CGTCTGA188800.05.432924794-95
ACGTCTG200750.05.30427794-95
CACGTCT214050.05.189585794-95
CACACGT236750.05.109713694-95
CCAGTCA122400.05.07321394-95
GCACACG253400.05.055328494-95
ACACGTC222000.05.034821594-95