FastQCFastQC Report
Thu 11 Sep 2014
lane4_Undetermined_L004_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane4_Undetermined_L004_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3401938
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTTAG2750.031.0386668
CCCGCTG3000.023.7033357
ACCTACT3450.023.297256
CCTACTA3750.021.4910247
CGTTCGA2205.9621016E-821.4804954
ACCCGCT4050.019.8458066
AGACGTT11650.017.095698
TGGAGCG4801.8189894E-1216.7371353
CTCACAT11050.016.6809671
CTCGCAT2859.0561844E-716.583421
CAACCTA6650.016.3445574
GACCCGC4358.731149E-1116.3035935
AGCGATC4358.731149E-1116.3033496
AGACCCG4651.4551915E-1116.2605044
TACTGTC2658.342013E-616.0495784
TGTTAGC5850.015.4014279
ACGGTTC3152.6096732E-614.9818632
AAGACGT14300.014.9181837
CGTGGAG5501.2732926E-1114.6084831
TGGAACG8500.014.4553253
Produced by FastQC (version 0.11.2)