FastQCFastQC Report
Thu 11 Sep 2014
lane4_Undetermined_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane4_Undetermined_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3401938
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAATGTAATTATGATAGAGTTTGATGATTCTTCTGAGTTTGCATTCC40610.11937313378433116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGTT751.3961107E-744.2016681
ATGCCGT69500.029.8279194
CTCGGTT2400.029.599331
GGGGACT3600.028.9249763
TATGCCG74000.027.7594493
CGGGGCT700.008516503527.0622441
TGGGGAC3750.026.5065752
GCCGTCT80350.025.9768456
TGCCGTC85650.024.4246625
GTATGCC90550.023.0523782
TCGGTTT3600.022.3517742
CGTATGC93250.022.244731
GACTTAC4600.021.6070336
CGGGCCT1554.00001E-521.3879031
GGGCTTA2151.1179181E-619.8124284
CGTCTTC108700.019.6807928
GACCGGC1300.00772443918.2037266
GTCTTCT123600.017.5770919
AATGTAA29150.017.5355285
GGACTAC1350.00960057617.5295145
Produced by FastQC (version 0.11.2)