FastQCFastQC Report
Thu 11 Sep 2014
lane3_Undetermined_L003_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane3_Undetermined_L003_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3628561
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN712981.9649111589966382No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACTA3450.030.1803287
CGTTCGA2800.026.9042224
ACCTACT4300.024.0917576
TACTGTC2853.6379788E-1223.128194
CGTACTG2704.1836756E-1122.7876727
CGCGGTC3001.8735591E-1020.4183961
CCCGCTG3601.0186341E-1018.4054287
AGCGATC4950.018.0743486
CTGTTAG4002.3646862E-1117.791998
TGGAGCG4850.017.4738773
ACTGTCG4053.092282E-1117.4401635
CGTGGAG5550.016.1309551
GCGGTCG3855.340553E-915.8743892
GTGGAGC5350.015.81732752
CAACCTA6900.015.694134
CCGGTTT3650.015.60777424-25
GACCCGC4251.1550583E-915.5114845
TACTACG6700.014.8792639
TGGAACG10200.014.7490552
ACCCGCT4553.0267984E-914.48874956
Produced by FastQC (version 0.11.2)