FastQCFastQC Report
Thu 11 Sep 2014
lane3_Undetermined_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane3_Undetermined_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3628561
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAATGTAATTATGATAGAGTTTGATGATTCTTCTGAGTTTGCATTCC41850.11533497714383194No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCCC250.005302498656.2229961
CCGGGCT555.38429E-542.5931781
CCGGGAT1055.260881E-835.697141
CCGGGTA702.2266516E-433.4660721
GCCCTAT758.2945917E-1032.09217566-67
CCCTATG701.5004844E-830.9460366-67
CGAGCCC701.5377736E-830.86796662-63
CTCGGTT3150.026.7728561
CCAGGGA1954.856338E-721.6090325
CTCGGGG1006.0375896E-721.12600916-17
CCTCGGG1059.620453E-720.1257114-15
TGGGGAC4450.019.993962
GGGGACT4250.019.8324723
AGCCCTA1151.8255014E-618.82417764-65
CGCGGCT1508.472253E-418.7409971
TCGGTTT6000.018.7311822
CACCCGG650.007078628518.48172470-71
TCCAGGG2352.9038838E-617.9311454
GACTCCG3700.017.28864752-53
CCTATGC1305.911701E-616.64249668-69
Produced by FastQC (version 0.11.2)