##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename lane2_Undetermined_L002_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3905782 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.27591145639977 33.0 33.0 33.0 15.0 33.0 2 28.30635401566191 33.0 33.0 33.0 15.0 33.0 3 28.31745960220002 33.0 33.0 33.0 15.0 33.0 4 31.49651875091851 37.0 33.0 37.0 15.0 37.0 5 31.492770973904843 37.0 33.0 37.0 15.0 37.0 6 31.285594792540905 37.0 33.0 37.0 6.0 37.0 7 31.241070290149324 37.0 33.0 37.0 6.0 37.0 8 31.17978320346604 37.0 33.0 37.0 6.0 37.0 9 31.127384477679502 37.0 33.0 37.0 6.0 37.0 10-11 31.13690216197422 37.0 33.0 37.0 6.0 37.0 12-13 31.09608549581108 37.0 33.0 37.0 6.0 37.0 14-15 32.77434301248764 40.0 33.0 40.0 6.0 40.0 16-17 32.74777547748441 40.0 33.0 40.0 6.0 40.0 18-19 32.708392967144604 40.0 33.0 40.0 6.0 40.0 20-21 32.66113636142519 40.0 33.0 40.0 6.0 40.0 22-23 32.54281344939375 40.0 33.0 40.0 6.0 40.0 24-25 32.43726569992898 40.0 33.0 40.0 6.0 40.0 26-27 32.271429639442246 40.0 33.0 40.0 6.0 40.0 28-29 32.1573661817275 40.0 33.0 40.0 6.0 40.0 30-31 32.08098775098047 40.0 33.0 40.0 6.0 40.0 32-33 31.988629933775105 38.5 33.0 40.0 6.0 40.0 34-35 31.985033342874743 38.5 33.0 40.0 6.0 40.0 36-37 32.09536604961567 40.0 33.0 40.0 6.0 40.0 38-39 32.075077666905116 40.0 33.0 40.0 6.0 40.0 40-41 32.001776340819845 40.0 33.0 40.0 6.0 40.0 42-43 31.883907755220335 37.0 33.0 40.0 6.0 40.0 44-45 31.757285992920238 37.0 33.0 40.0 6.0 40.0 46-47 31.64815483813485 37.0 33.0 40.0 6.0 40.0 48-49 31.530426685360318 37.0 30.0 40.0 6.0 40.0 50-51 30.716848636201405 37.0 27.0 38.5 6.0 40.0 52-53 30.864008667150394 37.0 30.0 38.5 6.0 40.0 54-55 31.53575058720635 37.0 33.0 40.0 6.0 40.0 56-57 31.48061873396928 37.0 33.0 40.0 6.0 40.0 58-59 31.330183174585784 37.0 33.0 40.0 6.0 40.0 60-61 31.106748149282268 37.0 27.0 40.0 6.0 40.0 62-63 30.86809926923725 37.0 27.0 38.5 6.0 40.0 64-65 30.65218104850706 37.0 27.0 37.0 6.0 40.0 66-67 30.412202473153904 37.0 27.0 37.0 6.0 40.0 68-69 30.1767466796662 37.0 27.0 37.0 6.0 40.0 70-71 29.960843180699793 37.0 27.0 37.0 6.0 37.0 72-73 29.777512288192224 37.0 27.0 37.0 6.0 37.0 74-75 29.604830735560768 37.0 27.0 37.0 6.0 37.0 76-77 29.45079781718488 37.0 27.0 37.0 6.0 37.0 78-79 29.332629803711523 37.0 27.0 37.0 6.0 37.0 80-81 29.21689036920135 37.0 27.0 37.0 6.0 37.0 82-83 29.093521732651745 35.0 27.0 37.0 6.0 37.0 84-85 28.953195160405777 33.0 27.0 37.0 6.0 37.0 86-87 28.872552794805237 33.0 27.0 37.0 6.0 37.0 88-89 28.78734757853869 33.0 27.0 37.0 6.0 37.0 90-91 28.643589171131417 33.0 27.0 37.0 6.0 37.0 92-93 28.571909799369244 33.0 27.0 37.0 6.0 37.0 94-95 28.484239647783724 33.0 27.0 37.0 6.0 37.0 96-97 28.355284677946695 33.0 22.0 37.0 6.0 37.0 98-99 28.27431139269934 33.0 22.0 37.0 6.0 37.0 100-101 27.6539910573606 33.0 22.0 37.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 1.7564740885313128 2306 2 1.693275212006185 2306 3 1.590368207625982 2306 4 1.870030852233029 2306 5 1.90909131820219 2306 6 2.067898554359406 2306 7 2.1044553311445853 2306 8 2.129784612866718 2306 9 2.186625710310228 2306 10-11 2.192707270682906 2306 12-13 2.1453993007442484 2306 14-15 2.36364531008811 2306 16-17 2.309660941390433 2306 18-19 2.4361071282327558 2306 20-21 2.36370893560116 2306 22-23 2.1397946203502443 2306 24-25 2.1233053631536762 2306 26-27 2.090175715311524 2306 28-29 2.2317498592403453 2306 30-31 2.1160374340383257 2306 32-33 1.9969701670002138 2306 34-35 2.0971649531257697 2306 36-37 2.1792186410004035 2306 38-39 2.1283465942206092 2306 40-41 2.0112892659397374 2306 42-43 2.0412232453021772 2306 44-45 1.9998746257011355 2306 46-47 1.9697509479668938 2306 48-49 2.0040099430796516 2306 50-51 1.8465436328696008 2306 52-53 1.9365819081492468 2306 54-55 1.9584509336308038 2306 56-57 1.9205230864558693 2306 58-59 1.8197352562660747 2306 60-61 1.7933257175268729 2306 62-63 1.8711736186153658 2306 64-65 1.6807488976220277 2306 66-67 1.6033792718917255 2306 68-69 1.506950456185642 2306 70-71 1.4455884767676537 2306 72-73 1.4647384757196704 2306 74-75 1.5047849524634884 2306 76-77 1.5348528975779843 2306 78-79 1.3450011286805896 2306 80-81 1.2598720294950922 2306 82-83 1.2968266084224673 2306 84-85 1.2183857837115006 2306 86-87 1.2105632262183548 2306 88-89 1.2488674799865294 2306 90-91 1.142935827750808 2306 92-93 1.0408441840416849 2306 94-95 1.0232526279347454 2306 96-97 0.8245704181160853 2306 98-99 0.7488882812231772 2306 100-101 0.5688733640423429 2307 1 1.4396651195955954 2307 2 1.434693390364128 2307 3 1.3986139536964508 2307 4 1.6316801744589995 2307 5 1.5675866339056093 2307 6 1.7459891952819042 2307 7 1.8418509833561103 2307 8 1.8016987297708447 2307 9 1.8711359569386623 2307 10-11 1.857063796258391 2307 12-13 1.8260872824741945 2307 14-15 1.919776418488702 2307 16-17 1.87487820105531 2307 18-19 1.9846685344732933 2307 20-21 1.8751705399402923 2307 22-23 1.8146975174341264 2307 24-25 1.7242694718397331 2307 26-27 1.675941595639209 2307 28-29 1.777352329849954 2307 30-31 1.759414539376607 2307 32-33 1.7160391035827018 2307 34-35 1.7222594608637323 2307 36-37 1.7756286445471474 2307 38-39 1.79671915799711 2307 40-41 1.8013376227594335 2307 42-43 1.7292625016971286 2307 44-45 1.7720508555993142 2307 46-47 1.7191367558026016 2307 48-49 1.7192933797875867 2307 50-51 1.5257649064100285 2307 52-53 1.6222370608754737 2307 54-55 1.6831306453930104 2307 56-57 1.6133309827179438 2307 58-59 1.6236980933148395 2307 60-61 1.4913442990024954 2307 62-63 1.532923720259845 2307 64-65 1.4743803304198941 2307 66-67 1.4794854665819983 2307 68-69 1.382844278796565 2307 70-71 1.339719048831423 2307 72-73 1.376757178303599 2307 74-75 1.2826684116629643 2307 76-77 1.283238696763096 2307 78-79 1.3220952211900432 2307 80-81 1.1698271329129426 2307 82-83 1.2528707783547297 2307 84-85 1.1697002414490214 2307 86-87 1.1301360598933847 2307 88-89 1.0747626001865314 2307 90-91 0.9645112739051882 2307 92-93 1.0228444608606857 2307 94-95 0.9871748936982918 2307 96-97 0.9397007393126344 2307 98-99 0.8834089085642418 2307 100-101 0.7693136792699811 2201 1 1.5268188340907187 2201 2 1.5087920941546429 2201 3 1.4811120647711284 2201 4 1.6993129254470674 2201 5 1.690034781752491 2201 6 1.8017694744822528 2201 7 1.7614692233724796 2201 8 1.773297678849108 2201 9 1.8157685997485409 2201 10-11 1.829657020278642 2201 12-13 1.823539476520704 2201 14-15 1.7333558837530205 2201 16-17 1.7102374574602521 2201 18-19 1.6823767921587631 2201 20-21 1.698431491723845 2201 22-23 1.5842015414111117 2201 24-25 1.6283850648448919 2201 26-27 1.5451642305411823 2201 28-29 1.6209831084729345 2201 30-31 1.6336454469748318 2201 32-33 1.6486885383307168 2201 34-35 1.6150693431507648 2201 36-37 1.6551139511819954 2201 38-39 1.5750594530300575 2201 40-41 1.502738278705003 2201 42-43 1.6052550365636336 2201 44-45 1.4871914830495676 2201 46-47 1.558697183383174 2201 48-49 1.6629171142495522 2201 50-51 1.5125926188103556 2201 52-53 1.6155280845178588 2201 54-55 1.6281018691566622 2201 56-57 1.5063082896177882 2201 58-59 1.5258965424253894 2201 60-61 1.4194129326113618 2201 62-63 1.431714933842997 2201 64-65 1.478193863091807 2201 66-67 1.5154591583177677 2201 68-69 1.3434657415165994 2201 70-71 1.4277792378329757 2201 72-73 1.4417010723996704 2201 74-75 1.2885544371376696 2201 76-77 1.2905724650454218 2201 78-79 1.2714998637953094 2201 80-81 1.1772772137111467 2201 82-83 1.2060536070382213 2201 84-85 1.1674722320199074 2201 86-87 0.9986321399016376 2201 88-89 0.9963775244156778 2201 90-91 1.0390072180617622 2201 92-93 1.0907575526855204 2201 94-95 0.9104819260351853 2201 96-97 0.8503224955393804 2201 98-99 0.7363781482225313 2201 100-101 0.7600037303149918 1101 1 1.3342001348511197 1101 2 1.312486188571338 1101 3 1.30620614958627 1101 4 1.6074615157924939 1101 5 1.5380170981905614 1101 6 1.6404101028414964 1101 7 1.6390608608344124 1101 8 1.6279186365417253 1101 9 1.5555048491719283 1101 10-11 1.6652001030878871 1101 12-13 1.578879298557947 1101 14-15 1.452696653422116 1101 16-17 1.482446274611135 1101 18-19 1.5258072403635907 1101 20-21 1.5012504877767796 1101 22-23 1.4090822003283492 1101 24-25 1.5155213798093286 1101 26-27 1.5181572443653337 1101 28-29 1.499727976336736 1101 30-31 1.4557839222894344 1101 32-33 1.488552321469129 1101 34-35 1.5156718408077765 1101 36-37 1.4920821345466706 1101 38-39 1.5050714132295155 1101 40-41 1.4068698701212128 1101 42-43 1.4872477352062603 1101 44-45 1.4744788137960505 1101 46-47 1.4597813243256752 1101 48-49 1.439355834403841 1101 50-51 1.4508791375991414 1101 52-53 1.3717090863776455 1101 54-55 1.4569509973979713 1101 56-57 1.431832565900482 1101 58-59 1.4284141331026952 1101 60-61 1.351886335256797 1101 62-63 1.427373360565383 1101 64-65 1.3675359268837077 1101 66-67 1.2687330802821677 1101 68-69 1.2959067284730956 1101 70-71 1.3120042983123419 1101 72-73 1.2174786419118107 1101 74-75 1.2532718487250776 1101 76-77 1.2849545109700813 1101 78-79 1.1968712457306019 1101 80-81 1.287452984810045 1101 82-83 1.2006871719917065 1101 84-85 1.2469225011765808 1101 86-87 1.2105050904390104 1101 88-89 1.164196029954315 1101 90-91 1.1883938664715572 1101 92-93 1.208850954955242 1101 94-95 1.0605025902541492 1101 96-97 1.0048376273833597 1101 98-99 0.9403402512973251 1101 100-101 0.897765190085515 2304 1 1.0294967263512653 2304 2 0.954932978976192 2304 3 0.9628401506010711 2304 4 1.0387473739140276 2304 5 1.0105879714148536 2304 6 1.176687109265938 2304 7 1.1510939198950325 2304 8 1.2707449444535364 2304 9 1.2192771513925642 2304 10-11 1.1533843997159785 2304 12-13 1.1462554264932017 2304 14-15 1.1245092074031575 2304 16-17 0.9202093799741675 2304 18-19 0.9741130112247234 2304 20-21 1.0444705490785609 2304 22-23 0.9525232757021769 2304 24-25 1.0262836278381755 2304 26-27 1.0140373480089266 2304 28-29 1.077226055685479 2304 30-31 0.9967489083796224 2304 32-33 1.013167197092045 2304 34-35 1.0093479275464574 2304 36-37 1.068257390196088 2304 38-39 0.9041406727886354 2304 40-41 1.0325227722530954 2304 42-43 1.1102025796215855 2304 44-45 0.9899161576188753 2304 46-47 1.0495920904706821 2304 48-49 0.9776121057477241 2304 50-51 0.9231758706628135 2304 52-53 1.036040405479337 2304 54-55 0.9168375397962443 2304 56-57 0.9480937617464669 2304 58-59 0.9900165368196134 2304 60-61 1.0083975418393472 2304 62-63 1.0375971104719355 2304 64-65 0.9773444502806612 2304 66-67 0.945491132304376 2304 68-69 1.0241135002997268 2304 70-71 0.9107150363036993 2304 72-73 0.8995504957418987 2304 74-75 0.8239289102797613 2304 76-77 0.8915013470252013 2304 78-79 0.7494589157062528 2304 80-81 0.7288885503404394 2304 82-83 0.8398221711089207 2304 84-85 0.827274199064103 2304 86-87 0.7094877417489549 2304 88-89 0.6684472304607638 2304 90-91 0.7287731931063384 2304 92-93 0.5970330247412932 2304 94-95 0.532268062432145 2304 96-97 0.7172505220712253 2304 98-99 0.7007317301272167 2304 100-101 0.7184513596468101 2202 1 1.1099663725947977 2202 2 1.0362040986197059 2202 3 1.008664606855607 2202 4 1.1696700438271677 2202 5 1.147280937646837 2202 6 1.2603142641524236 2202 7 1.0687060442287688 2202 8 1.0861828673341627 2202 9 0.944796314176731 2202 10-11 1.0578502532032026 2202 12-13 0.9997387531931103 2202 14-15 0.9901976394964365 2202 16-17 0.9796858079157005 2202 18-19 0.9869701429332594 2202 20-21 1.0084772137161764 2202 22-23 0.9568818238413073 2202 24-25 1.0224655084521999 2202 26-27 1.014566851126638 2202 28-29 1.0883760568560845 2202 30-31 0.998128660055535 2202 32-33 1.0780447909549622 2202 34-35 0.9775691560561199 2202 36-37 1.012165568034213 2202 38-39 0.989449985332044 2202 40-41 1.0940106877198943 2202 42-43 1.0084591603659305 2202 44-45 0.9650127324321538 2202 46-47 0.9774278781699763 2202 48-49 0.9936513287789666 2202 50-51 0.9397211847358129 2202 52-53 0.9403033543083374 2202 54-55 1.125226086608695 2202 56-57 1.0653437793055254 2202 58-59 1.020467843603651 2202 60-61 1.0594194789545064 2202 62-63 0.9671279324699213 2202 64-65 0.9706657654150099 2202 66-67 0.7732871194745599 2202 68-69 0.9148224179594564 2202 70-71 0.8657876527592663 2202 72-73 0.920056742864162 2202 74-75 0.9264078589214719 2202 76-77 0.7915875815049453 2202 78-79 0.7288000998890531 2202 80-81 0.8192006333128354 2202 82-83 0.9538038628655272 2202 84-85 0.9304484863614881 2202 86-87 0.8021390813098996 2202 88-89 0.6947705980537684 2202 90-91 0.8311912703505833 2202 92-93 0.8145222356762929 2202 94-95 0.5074672601783412 2202 96-97 0.6341111187670023 2202 98-99 0.6986359591285343 2202 100-101 0.7863713527792697 1102 1 1.12284343188713 1102 2 1.0231723763289438 1102 3 0.9641675163395433 1102 4 1.1655203446012123 1102 5 1.2170208490704262 1102 6 1.051177138118458 1102 7 0.804654455930752 1102 8 0.8630041606079608 1102 9 0.7970098146188853 1102 10-11 0.7735199803776744 1102 12-13 0.8272378343129496 1102 14-15 0.8524272976730103 1102 16-17 0.9094877301038338 1102 18-19 0.8718704853753323 1102 20-21 0.8391476141295797 1102 22-23 0.8281758254643634 1102 24-25 0.7751693815775909 1102 26-27 0.8634234366771096 1102 28-29 0.8352405829868488 1102 30-31 0.9069131544531004 1102 32-33 0.8043995478429267 1102 34-35 0.7856860521698934 1102 36-37 0.8496869934084117 1102 38-39 0.8725265780879923 1102 40-41 0.8888405766172696 1102 42-43 0.7666126297299201 1102 44-45 0.8437025366833915 1102 46-47 0.7591527163196901 1102 48-49 0.7368174077262353 1102 50-51 0.8292000978006477 1102 52-53 0.8229188693725149 1102 54-55 0.7958992527604245 1102 56-57 0.8492231640015326 1102 58-59 0.8386618528688707 1102 60-61 0.7798333571382905 1102 62-63 0.8087139014427045 1102 64-65 0.718620210793496 1102 66-67 0.6987721244419696 1102 68-69 0.8178841994588488 1102 70-71 0.7210729423065949 1102 72-73 0.7601954300846323 1102 74-75 0.7358632268192551 1102 76-77 0.7005827814932992 1102 78-79 0.7236258037876517 1102 80-81 0.7398459601248497 1102 82-83 0.718638955717914 1102 84-85 0.7681613299407921 1102 86-87 0.6723020415232988 1102 88-89 0.6663302561211708 1102 90-91 0.7317871777856872 1102 92-93 0.6183576960807464 1102 94-95 0.6463384386376063 1102 96-97 0.6212981081516631 1102 98-99 0.6567490341564657 1102 100-101 0.540578550249343 2305 1 1.1576384552999066 2305 2 1.1639873245971053 2305 3 1.105259026075128 2305 4 1.2728764653104534 2305 5 1.3186116472040332 2305 6 1.1080715979811409 2305 7 0.872285439377805 2305 8 0.9829345403154583 2305 9 0.9124810981472145 2305 10-11 0.9628942540153922 2305 12-13 1.0177420048322539 2305 14-15 1.1044906671929695 2305 16-17 1.0623584450411272 2305 18-19 1.0401734683031023 2305 20-21 1.0131947545668751 2305 22-23 1.06816146912751 2305 24-25 1.1146984398667996 2305 26-27 1.1480858072611895 2305 28-29 1.1936151128150954 2305 30-31 1.171543773985313 2305 32-33 1.105685955727239 2305 34-35 1.1665260394363273 2305 36-37 1.1297483962690436 2305 38-39 1.1887437834568075 2305 40-41 1.0993485312984816 2305 42-43 1.094114578780463 2305 44-45 1.131248535311979 2305 46-47 1.0750416387381563 2305 48-49 1.1308108103023642 2305 50-51 1.0386113360709572 2305 52-53 1.0873745749978632 2305 54-55 1.1433281978700442 2305 56-57 1.196517177498837 2305 58-59 1.1220848068491769 2305 60-61 1.087576974645149 2305 62-63 1.118763544398746 2305 64-65 1.1358909392653764 2305 66-67 1.1591509135239733 2305 68-69 1.1062957215412972 2305 70-71 1.094720328852521 2305 72-73 1.0875420075242594 2305 74-75 1.0382404124662337 2305 76-77 1.1353765619331888 2305 78-79 1.1001032324358313 2305 80-81 1.0770289761433176 2305 82-83 1.1165368814033272 2305 84-85 1.0575165101338833 2305 86-87 1.060120285741231 2305 88-89 1.1115155315881324 2305 90-91 1.079416621327443 2305 92-93 1.1177748108506442 2305 94-95 1.0926863426055213 2305 96-97 1.022154792687214 2305 98-99 0.9272715523366699 2305 100-101 1.0226193529059913 2203 1 0.8039469468471623 2203 2 0.9081674055408371 2203 3 0.8104431703726895 2203 4 1.107528951554869 2203 5 1.1371818208935487 2203 6 0.5498786048414139 2203 7 0.4378882600136045 2203 8 0.6444920604840298 2203 9 0.5324784305608148 2203 10-11 0.5450051905807278 2203 12-13 0.5711280207515479 2203 14-15 0.7293601171312574 2203 16-17 0.6646418585816889 2203 18-19 0.6792289541002248 2203 20-21 0.6862114444963296 2203 22-23 0.6414086762453834 2203 24-25 0.738072492065271 2203 26-27 0.816479960666264 2203 28-29 0.7475638816835541 2203 30-31 0.7659576055555206 2203 32-33 0.6648475656560535 2203 34-35 0.6550310997668944 2203 36-37 0.7033355859263537 2203 38-39 0.6768480995577093 2203 40-41 0.7037093614298158 2203 42-43 0.7515298627032649 2203 44-45 0.7525713078773286 2203 46-47 0.725855926223133 2203 48-49 0.7115111595136767 2203 50-51 0.7292862354301981 2203 52-53 0.7257081283846212 2203 54-55 0.7412129231086446 2203 56-57 0.7936250692433298 2203 58-59 0.7390845400905484 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1213 28-29 -3.866696934608484 1213 30-31 -3.711936597587961 1213 32-33 -3.6268786047005506 1213 34-35 -3.7308813136182692 1213 36-37 -3.828470016574915 1213 38-39 -3.9694591507380466 1213 40-41 -3.9504249126955884 1213 42-43 -4.128821244276622 1213 44-45 -4.048074078298097 1213 46-47 -4.20894422588097 1213 48-49 -3.5858608195297528 1213 50-51 -3.6249177530774936 1213 52-53 -3.526939961863814 1213 54-55 -3.4829409977972183 1213 56-57 -3.496816666433535 1213 58-59 -3.4536788957722244 1213 60-61 -3.4070061408802026 1213 62-63 -3.4535078214929555 1213 64-65 -3.336649187391746 1213 66-67 -3.394387484237676 1213 68-69 -3.285529837832442 1213 70-71 -3.2732842322608313 1213 72-73 -3.0937571599493694 1213 74-75 -3.174179516321267 1213 76-77 -3.1405732593928555 1213 78-79 -3.048570852556413 1213 80-81 -3.1813074279254785 1213 82-83 -3.0013309427163684 1213 84-85 -3.222970483516125 1213 86-87 -3.0384366649756096 1213 88-89 -2.9189038701754697 1213 90-91 -3.028757979976838 1213 92-93 -3.0765717330722957 1213 94-95 -2.9712387642989206 1213 96-97 -3.13536145721638 1213 98-99 -3.0254881997478584 1213 100-101 -2.8782864962023496 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 88595.0 3 13780.0 4 5217.0 5 2708.0 6 12728.0 7 89182.0 8 140328.0 9 148112.0 10 88168.0 11 49010.0 12 34457.0 13 25897.0 14 20551.0 15 18266.0 16 16708.0 17 16108.0 18 15859.0 19 15490.0 20 15713.0 21 15483.0 22 15381.0 23 15225.0 24 15564.0 25 15842.0 26 16583.0 27 18697.0 28 21741.0 29 26251.0 30 32667.0 31 42372.0 32 58270.0 33 85171.0 34 132178.0 35 231453.0 36 530698.0 37 1323228.0 38 491939.0 39 162.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 22.197806354207763 29.437079117393512 20.910816357942814 27.454298170455914 2 22.607612130141757 33.03362373831844 26.670918707707408 17.687845423832393 3 21.523908281594384 31.513188532220138 25.474797090458274 21.488106095727204 4 23.16284942708728 32.373712155474635 22.963146928649294 21.500291488788793 5 23.78061877333141 33.81104754669159 23.032103912521265 19.376229767455733 6 22.177403590452574 33.62543139718768 23.389500790178747 20.807664222181 7 21.243321118370293 27.906813680408455 29.63469609742698 21.21516910379427 8 21.824848286714722 29.146637079115862 25.318668854547617 23.7098457796218 9 22.22637320888804 29.11729502739607 25.375031253153306 23.281300510562577 10-11 22.67038578315161 32.35105275160522 23.56748739215303 21.411074073090134 12-13 22.008772225392807 30.543502066964162 25.726425236547882 21.72130047109514 14-15 22.142349373993532 31.024967720407954 25.30709465207282 21.525588253525697 16-17 22.48186486233978 31.144404966265064 24.84305326286208 21.53067690853307 18-19 22.41717547359094 30.94695283855751 24.925859186359116 21.710012501492425 20-21 22.530970841058487 31.008817208831957 24.801428451036575 21.65878349907298 22-23 22.624993807230414 31.135690696720836 24.651175782950542 21.588139713098208 24-25 22.609341366251883 31.002399907811473 24.689819628508722 21.698439097427922 26-27 22.74570129802817 31.105263317545344 24.654266020798048 21.49476936362844 28-29 22.83298877731837 31.05616087250021 24.40288530503755 21.70796504514387 30-31 22.548140049245664 30.887360763120554 24.688179801261022 21.876319386372764 32-33 22.729416736640754 31.009943047292985 24.64525578331538 21.61538443275088 34-35 22.77463493103945 30.869259686749746 24.568010306215132 21.78809507599567 36-37 22.583224485170508 30.646699905318258 24.754651337819887 22.015424271691348 38-39 22.685502111797636 30.711982200609896 24.75894900221437 21.8435666853781 40-41 22.473741868635805 30.822709713843256 24.615640315869243 22.0879081016517 42-43 22.579454578407752 30.764722533893295 24.696629652190914 21.959193235508042 44-45 22.619076825026006 30.8000482218967 24.690666293131624 21.890208659945674 46-47 22.63477349777982 30.799600106279485 24.59480945549705 21.970816940443648 48-49 22.366280474217607 30.751569284751284 24.73990158220738 22.14224865882373 50-51 22.41892097663498 30.4922258666977 24.968457209872966 22.120395946794353 52-53 22.518344787649426 30.074247511677015 24.93861756124354 22.46879013943002 54-55 22.372751761831687 30.025045636795113 25.088736563159042 22.513466038214162 56-57 22.483775445301017 30.05129308183404 25.142081968640884 22.32284950422406 58-59 22.505719149481948 30.071398156386625 25.048654170680773 22.374228523450657 60-61 22.426156077564986 29.96482837169218 25.029740812154472 22.579274738588357 62-63 22.452676906832092 30.04629522693901 25.076755044463876 22.424272821765026 64-65 22.49316162634382 29.85231541274267 25.076487107062416 22.578035853851095 66-67 22.148430740509067 30.00208681107681 25.147974176339893 22.701508272074232 68-69 22.299879449625184 29.973310001854113 25.160163082816855 22.56664746570385 70-71 22.180057706082263 29.968795002448967 25.100509181406977 22.750638110061793 72-73 22.121906560415507 29.844011308972224 25.179634399896837 22.854447730715428 74-75 22.26101118410423 29.83489955848608 25.20730615257733 22.696783104832367 76-77 22.12284681609013 30.17494747140726 25.064632806723026 22.637572905779585 78-79 21.98099484857035 29.923980977956038 25.27872013750519 22.816304035968418 80-81 22.197584340935723 30.060386857554082 25.10083062447879 22.641198177031406 82-83 22.12345860808355 30.208391885576187 25.016473797228073 22.651675709112194 84-85 22.10299200171024 30.08359868934146 25.137169151753724 22.67624015719457 86-87 22.075092536369528 30.244463357682843 25.132055770327415 22.54838833562021 88-89 22.0892567314852 30.156454521051046 25.196346807785613 22.55794193967814 90-91 21.954906822643363 30.09761826940447 25.287313398862583 22.660161509089587 92-93 22.14579311158625 29.924676247246484 25.23259262622605 22.696938014941207 94-95 22.056902517821385 30.15136018529041 25.13383940274781 22.657897894140394 96-97 21.814654638703033 30.04152356200774 25.356750707349974 22.78707109193926 98-99 21.95553871982768 30.100521614258863 25.189454733596605 22.754484932316853 100-101 22.005131358582553 30.377621690925068 24.941573019174555 22.675673931317824 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 79865.0 1 43313.5 2 7476.5 3 7213.0 4 5057.5 5 3203.0 6 2309.5 7 1892.5 8 1550.0 9 1323.0 10 1169.0 11 1043.0 12 1015.0 13 1022.0 14 1050.5 15 1106.0 16 1219.0 17 1425.0 18 1656.0 19 1961.0 20 2338.0 21 2764.5 22 3446.0 23 4379.0 24 5518.0 25 6913.0 26 8747.0 27 10924.0 28 13634.0 29 16788.0 30 20306.5 31 24621.5 32 29326.0 33 34188.0 34 44105.5 35 53764.0 36 63572.5 37 83200.0 38 108628.5 39 125513.0 40 141788.0 41 178469.5 42 213016.5 43 230382.0 44 245476.5 45 262848.0 46 273138.0 47 278705.0 48 255934.0 49 200617.5 50 158049.0 51 132010.5 52 109876.5 53 96386.5 54 86457.0 55 68239.5 56 45986.5 57 28907.5 58 19590.0 59 14765.0 60 11710.5 61 9459.0 62 7972.5 63 6851.0 64 5907.0 65 5163.0 66 4481.5 67 3827.0 68 3231.0 69 2787.0 70 2353.0 71 1964.0 72 1715.0 73 1424.0 74 1175.5 75 1020.5 76 872.0 77 761.0 78 657.5 79 573.5 80 484.5 81 381.5 82 317.0 83 286.5 84 255.5 85 204.0 86 155.5 87 136.0 88 109.5 89 72.5 90 54.5 91 43.0 92 31.0 93 17.5 94 10.0 95 7.5 96 6.5 97 3.5 98 4.0 99 6.5 100 18.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.5245648630671145 2 2.132837931046843 3 2.027762942222582 4 2.063402412116191 5 2.1356286653991443 6 2.4225366392696777 7 2.5061306545014546 8 2.575105318217965 9 2.820561925883216 10-11 2.6622837628930647 12-13 2.73886253764291 14-15 2.936338996902541 16-17 2.8527321801370378 18-19 2.750678353272149 20-21 2.7820805155024013 22-23 2.8428109914992694 24-25 2.9073051184116268 26-27 2.934329156107535 28-29 2.904975239273467 30-31 2.9129377932511336 32-33 2.9467338422881766 34-35 2.9756909115767343 36-37 2.962697354844689 38-39 2.9639263020824 40-41 2.951764845042555 42-43 2.9515984251041147 44-45 2.9436614741939002 46-47 2.964195134290649 48-49 2.9678691744700547 50-51 2.982373312181786 52-53 2.99646780081428 54-55 2.992960180573314 56-57 2.9897213925405977 58-59 2.983743076290484 60-61 3.0011147575568735 62-63 3.0107799155201187 64-65 3.0015756127710147 66-67 3.0136218560073242 68-69 2.9932418143152892 70-71 3.0069906615371775 72-73 3.007400310616414 74-75 3.018755271031512 76-77 3.017193483916921 78-79 2.9987592753512615 80-81 2.99879767995244 82-83 2.9809907465393612 84-85 2.9916416225995204 86-87 2.999053710626963 88-89 3.0008075207474456 90-91 2.984895214325838 92-93 3.0126489394441367 94-95 3.0100630296314543 96-97 3.022518921947001 98-99 3.0094357544788726 100-101 3.0085268455843157 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3905782.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.724886836675246 #Duplication Level Percentage of deduplicated Percentage of total 1 89.70849362550528 26.665748213067474 2 4.458717304873757 2.650697346481962 3 1.7569969240831713 1.566796042222763 4 0.8975301475061028 1.0671592826849339 5 0.43853348778473705 0.6517679149246908 6 0.29391478847732133 0.5241950296268231 7 0.2676796582907396 0.5569723285820494 8 0.18039035253180377 0.4289666252348657 9 0.1324487156347266 0.35433207755147045 >10 0.7619224739432755 4.252262648370619 >50 0.13539290955310157 3.113187577247687 >100 0.9643069756550293 55.47265456116695 >500 0.003395911933139963 0.6293872161401998 >1k 1.8871637626106735E-4 0.06186838550848057 >5k 0.0 0.0 >10k+ 8.800785173100448E-5 2.004004751189012 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 76605 1.9613229821838496 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 7.168858886645491E-4 0.0 0.0 2 8.449012259260757E-4 0.0 2.5603067452305326E-5 3 0.0012801533726152664 0.0 2.5603067452305326E-5 4 0.0030979711617289446 0.0 2.5603067452305326E-5 5 0.003686841713131967 0.0 2.5603067452305326E-5 6 0.004813376681033401 0.0 2.5603067452305326E-5 7 0.004966995085747233 0.0 2.5603067452305326E-5 8 0.005607071772054866 0.0 2.5603067452305326E-5 9 0.0059399116489348355 0.0 2.5603067452305326E-5 10-11 0.007168858886645492 0.0 2.5603067452305326E-5 12-13 0.009165898147925306 0.0 2.5603067452305326E-5 14-15 0.012865541394783426 0.0 2.5603067452305326E-5 16-17 0.016641993843998462 0.0 2.5603067452305326E-5 18-19 0.019432728196299744 0.0 2.5603067452305326E-5 20-21 0.02245389015567177 0.0 2.5603067452305326E-5 22-23 0.02423330334360699 0.0 2.5603067452305326E-5 24-25 0.028073763461452787 0.0 2.5603067452305326E-5 26-27 0.02976356591330494 1.2801533726152663E-5 2.5603067452305326E-5 28-29 0.031210139224360195 2.5603067452305326E-5 2.5603067452305326E-5 30-31 0.032554300265606226 2.5603067452305326E-5 2.5603067452305326E-5 32-33 0.0336936367672338 2.5603067452305326E-5 2.5603067452305326E-5 34-35 0.03482017173513524 2.5603067452305326E-5 2.5603067452305326E-5 36-37 0.03590830210185822 2.5603067452305326E-5 2.5603067452305326E-5 38-39 0.0365355772544397 2.5603067452305326E-5 2.5603067452305326E-5 40-41 0.03754689841880576 2.5603067452305326E-5 3.840460117845799E-5 42-43 0.03868623492043335 2.5603067452305326E-5 5.120613490461065E-5 44-45 0.039633548416168646 2.5603067452305326E-5 5.120613490461065E-5 46-47 0.04041444197346396 2.5603067452305326E-5 5.120613490461065E-5 48-49 0.042104244425316115 2.5603067452305326E-5 5.120613490461065E-5 50-51 0.04370443614108519 2.5603067452305326E-5 5.120613490461065E-5 52-53 0.045087001783509675 2.5603067452305326E-5 5.120613490461065E-5 54-55 0.04618793368395881 2.5603067452305326E-5 5.120613490461065E-5 56-57 0.04746808705657407 2.5603067452305326E-5 5.120613490461065E-5 58-59 0.04883785116527241 2.5603067452305326E-5 7.680920235691598E-5 60-61 0.05025882140887536 2.5603067452305326E-5 8.961073608306863E-5 62-63 0.05255029594585668 2.5603067452305326E-5 1.024122698092213E-4 64-65 0.05427850299888729 2.5603067452305326E-5 1.024122698092213E-4 66-67 0.056019511585644055 2.5603067452305326E-5 1.024122698092213E-4 68-69 0.05787573397593619 2.5603067452305326E-5 1.024122698092213E-4 70-71 0.06046164378861903 2.5603067452305326E-5 1.2801533726152664E-4 72-73 0.06321397353974186 2.5603067452305326E-5 1.408168709876793E-4 74-75 0.0658766925547816 2.5603067452305326E-5 1.5361840471383195E-4 76-77 0.0694739235318305 2.5603067452305326E-5 1.7922147216613727E-4 78-79 0.07319916984614092 2.5603067452305326E-5 2.1762607334459527E-4 80-81 0.07665558395220215 2.5603067452305326E-5 2.3042760707074793E-4 82-83 0.08098250235164176 2.5603067452305326E-5 2.3042760707074793E-4 84-85 0.08621832964563818 3.840460117845799E-5 2.3042760707074793E-4 86-87 0.09167178301297921 5.120613490461065E-5 2.3042760707074793E-4 88-89 0.09822616828076938 5.120613490461065E-5 2.3042760707074793E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGTTAG 330 0.0 31.65638 8 CGTTCGA 255 0.0 31.621782 4 TACTGTC 245 0.0 29.040413 4 CGTGGAG 480 0.0 25.694391 1 ACTGTCG 365 0.0 23.391764 5 ACCTACT 310 0.0 22.951597 6 CCTACTA 290 3.6379788E-12 22.909391 7 TGGAGCG 645 0.0 20.590929 3 GCGATCA 620 0.0 19.135147 7 GTGGAGC 620 0.0 18.327013 2 AGCGATC 625 0.0 18.214388 6 GCATTCC 795 0.0 17.899122 3 GAGCGAT 680 0.0 17.438713 5 CCCGTTC 220 5.456968E-12 17.279184 66-67 CTCACAT 1025 0.0 17.123165 1 TGTTAGC 615 0.0 16.212746 9 CTGTATG 300 0.0 15.838627 76-77 GTCTGAG 300 1.4890193E-6 15.811933 1 TCCCGTT 260 5.456968E-12 15.535062 64-65 CGAGTCT 225 6.935482E-4 14.729487 2 >>END_MODULE