FastQCFastQC Report
Thu 11 Sep 2014
lane2_Undetermined_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane2_Undetermined_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3905782
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN766051.9613229821838496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTTAG3300.031.656388
CGTTCGA2550.031.6217824
TACTGTC2450.029.0404134
CGTGGAG4800.025.6943911
ACTGTCG3650.023.3917645
ACCTACT3100.022.9515976
CCTACTA2903.6379788E-1222.9093917
TGGAGCG6450.020.5909293
GCGATCA6200.019.1351477
GTGGAGC6200.018.3270132
AGCGATC6250.018.2143886
GCATTCC7950.017.8991223
GAGCGAT6800.017.4387135
CCCGTTC2205.456968E-1217.27918466-67
CTCACAT10250.017.1231651
TGTTAGC6150.016.2127469
CTGTATG3000.015.83862776-77
GTCTGAG3001.4890193E-615.8119331
TCCCGTT2605.456968E-1215.53506264-65
CGAGTCT2256.935482E-414.7294872
Produced by FastQC (version 0.11.2)