FastQCFastQC Report
Thu 11 Sep 2014
lane2_Undetermined_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane2_Undetermined_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3905782
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAATGTAATTATGATAGAGTTTGATGATTCTTCTGAGTTTGCATTCC43010.11011879311236522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT89600.040.2433244
TATGCCG97450.037.052463
GCCGTCT102700.035.4292346
TGCCGTC106050.034.000965
CGAGCCC552.5469853E-630.5062962-63
GTATGCC120600.030.1729832
CGTATGC122300.029.9619121
GCCCTAT504.6017434E-528.81235166-67
CGTCTTC132100.027.8986438
CGGGTCT856.940203E-427.5638771
CGGGCCT1057.579892E-526.7763391
CGGGGAT1406.5448694E-726.7763371
CTCGGTT2500.026.2408141
GTCTTCT149200.024.8682639
CCGGGAT950.00132826424.6624161
CCGTCTT151600.024.2792077
CCTCGGG701.9578823E-523.46145214-15
CTCGGGG701.9603121E-523.45769116-17
GGGGACT2905.456968E-1222.6086483
TGGGGAC2905.456968E-1222.6086482
Produced by FastQC (version 0.11.2)